Tethered Fibronectin Liposomes on Supported Lipid Bilayers as a Pre-Packaged Controlled-Release Platform for Cell-Based Assays.
Summary of "Tethered Fibronectin Liposomes on Supported Lipid Bilayers as a Pre-Packaged Controlled-Release Platform for Cell-Based Assays."
A biomimetic construct containing an extracellular matrix protein-liposome composite tethered on supported lipid bilayers (SLBs) was formed with fibronectin (FN), and polyethylene glycol (PEG) and cholesterol-containing liposomes. The construct can serve as a multifunctional platform for cell attachment and drug release. The successful fabrication of the FN-liposome-SLB model platform was analyzed in situ with a quartz crystal microbalance with dissipation. The long term stability of the surface tethered liposomes was measured via an encapsulated fluorescent probe. Less than 20% of the fluorescent probe content was released in 8 days, which compared favorably to the release of 90% of the probe content in one day from a similar construct made without PEG and cholesterol. HeLa cells were used to study the cellular interactions with the model platform. The extracellular matrix composition, FN, was found to be essential to promote HeLa cell adhesion on the liposome-SLB surfaces. Upon cell adhesion, the liposomes were spatially reorganized and absorbed by the cells. The rate of HeLa cell apoptosis was correlated with the surface density of doxorubicin-loaded liposomes, confirming the effective drug delivery through liposomes. The multi-functional model platform could be useful as pre-administered, controlled-release platforms for cell and tissue-based assays.
Affiliation
Journal Details
This article was published in the following journal.
Name: Biomacromolecules
ISSN: 1526-4602
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22721275
- DOI: http://dx.doi.org/10.1021/bm300426u
Medical and Biotech [MESH] Definitions
Dimyristoylphosphatidylcholine
A synthetic phospholipid used in liposomes and lipid bilayers for the study of biological membranes.
1,2-dipalmitoylphosphatidylcholine
Synthetic phospholipid used in liposomes and lipid bilayers to study biological membranes. It is also a major constituent of PULMONARY SURFACTANTS.
Lipid Bilayers
Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.
Hospitals, Packaged
Hospital equipment and supplies, packaged for long-term storage, sufficient to set up a general hospital in an emergency situation. They are also called Packaged Disaster Hospitals and formerly Civil Defense Emergency Hospitals.
Employment, Supported
Paid work for mentally or physically disabled persons, taking place in regular or normal work settings. It may be competitive employment (work that pays minimum wage) or employment with subminimal wages in individualized or group placement situations. It is intended for persons with severe disabilities who require a range of support services to maintain employment. Supported employment differs from SHELTERED WORKSHOPS in that work in the latter takes place in a controlled working environment. Federal regulations are authorized and administered by the U.S. Department of Education, Office of Special Education and Rehabilitative Services.
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