In-line cold column trapping of organic phase in dispersive liquid-liquid microextraction: Enrichment and determination of curcumin in human serum.
Summary of "In-line cold column trapping of organic phase in dispersive liquid-liquid microextraction: Enrichment and determination of curcumin in human serum."
A new temperature controlled cold column trapping (CCT) system was developed for in-line sequestration of organic phase in dispersive liquid-liquid microextraction (DLLME) method. In the developed CCT-DLLME method, the dispersed organic extraction phase is solidified and trapped in the CCT, packed with glass particles. Subsequently, the sequestered phase is washed out in an elevated temperature by using an appropriate solvent. The column temperature is controlled by a pair of thermal electric cooler (TEC) plates. The new device is simple and portable and can eliminate the need for centrifugation in the DLLME method for solvents with an appropriate melting point. Some important parameters such as types of extraction and disperser solvents and their volumes, minimum and maximum column temperatures and extraction time were optimized for the extraction of curcumin, as a model compound. Using 1-dodecanol as the organic solvent and acetone as the disperser, recoveries exceeding 90% and a relative standard deviation of 2.87% were obtained for 5 replicated analyses of curcumin by an HPLC method. The detection limit of curcumin (3σ) extracted by the CCT-DLLME system was 28μgL(-1). The method was successfully applied to the determination of curcumin in some human serum samples.
Affiliation
Department of Chemistry, Faculty of Sciences, Lorestan University, Khoramabad, Iran.
Journal Details
This article was published in the following journal.
Name: Journal of chromatography. A
ISSN: 1873-3778
Pages: 14-9
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22609163
- DOI: http://dx.doi.org/10.1016/j.chroma.2012.04.059
Medical and Biotech [MESH] Definitions
Chromatography, Gas
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Chromatography
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Spin Trapping
A technique for detecting short-lived reactive FREE RADICALS in biological systems by providing a nitrone or nitrose compound for an addition reaction to occur which produces an ELECTRON SPIN RESONANCE SPECTROSCOPY-detectable aminoxyl radical. In spin trapping, the compound trapping the radical is called the spin trap and the addition product of the radical is identified as the spin adduct. (Free Rad Res Comm 1990;9(3-6):163)
Chromatography, Liquid
Chromatographic techniques in which the mobile phase is a liquid.
Gels
Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.
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