A highly thermoactive and salt-tolerant α-amylase isolated from a pilot-plant biogas reactor.
Summary of "A highly thermoactive and salt-tolerant α-amylase isolated from a pilot-plant biogas reactor."
Aiming at the isolation of novel enzymes from previously uncultured thermophilic microorganisms, a metagenome library was constructed from DNA isolated from a pilot-plant biogas reactor operating at 55 °C. The library was screened for starch-degrading enzymes, and one active clone was found. An open reading frame of 1,461 bp encoding an α-amylase from an uncultured organism was identified. The amy13A gene was cloned in Escherichia coli, resulting in high-level expression of the recombinant amylase. The novel enzyme Amy13A showed the highest sequence identity (75 %) to α-amylases from Petrotoga mobilis and Halothermothrix orenii. Amy13A is highly thermoactive, exhibiting optimal activity at 80 °C, and it is also highly salt-tolerant, being active in 25 % (w/v) NaCl. Amy13A is one of the few enzymes that tolerate high concentrations of salt and elevated temperatures, making it a potential candidate for starch processing under extreme conditions.
Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr. 12, Hamburg, 21073, Germany, firstname.lastname@example.org.
This article was published in the following journal.
Name: Applied microbiology and biotechnology
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22743714
- DOI: http://dx.doi.org/10.1007/s00253-012-4194-x
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