Characterization of Dental Epithelial Stem Cells from the Mouse Incisor with 2D and 3D Platforms.
Summary of "Characterization of Dental Epithelial Stem Cells from the Mouse Incisor with 2D and 3D Platforms."
Dental epithelial stem cells drive continuous growth in the adult mouse incisor. To date, no robust system for the primary culture of these cells has been reported, and little is known about the basic molecular architecture of these cells or the minimal extracellular scaffolding necessary to maintain the epithelial stem cell population in an undifferentiated state. We report a method to isolate dental epithelial stem cells from the cervical loop of the mouse mandibular incisor. Cells were viable in a two dimensional (2D) culture system and did not demonstrate preferential proliferation atop various substrates. Characterization of these cells indicated that E-cadherin, integrin alpha-6, and integrin beta-4 mark the dental epithelial stem cells both in vivo and in vitro. We also grew these cells in a three dimensional (3D) microenvironment and obtained spheres with epithelial morphology and expression patterns. Insights into the mechanisms of stem cell maintenance in vitro will help to lay the groundwork for the successful generation of bioengineered teeth from adult dental epithelial stem cells.
Affiliation
University of California, San Francisco, Department of Bioengineering and Therapeutic Sciences, 1700 4th Street, Mail Code 2520, San Francisco, California, United States, 94158-2230, 415-514-9695, 415-476-2414; miquella.chavez@ucsf.edu.
Journal Details
This article was published in the following journal.
Name: Tissue engineering. Part C, Methods
ISSN: 1937-3392
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22742471
- DOI: http://dx.doi.org/10.1089/ten.TEC.2012.0232
Medical and Biotech [MESH] Definitions
Multipotent Stem Cells
Specialized stem cells that are committed to give rise to cells that have a particular function; examples are MYOBLASTS; MYELOID PROGENITOR CELLS; and skin stem cells. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)
Peripheral Blood Stem Cell Transplantation
Transplantation of stem cells collected from the peripheral blood. It is a less invasive alternative to direct marrow harvesting of hematopoietic stem cells. Enrichment of stem cells in peripheral blood can be achieved by inducing mobilization of stem cells from the BONE MARROW.
Wilms Tumor
A malignant kidney tumor, caused by the uncontrolled multiplication of renal stem (blastemal), stromal (STROMAL CELLS), and epithelial (EPITHELIAL CELLS) elements. However, not all three are present in every case. Several genes or chromosomal areas have been associated with Wilms tumor which is usually found in childhood as a firm lump in a child's side or ABDOMEN.
Embryonal Carcinoma Stem Cells
The malignant stem cells of TERATOCARCINOMAS, which resemble pluripotent stem cells of the BLASTOCYST INNER CELL MASS. The EC cells can be grown in vitro, and experimentally induced to differentiate. They are used as a model system for studying early embryonic cell differentiation.
Lymphopoiesis
Formation of LYMPHOCYTES and PLASMA CELLS from the lymphoid stem cells which develop from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW. These lymphoid stem cells differentiate into T-LYMPHOCYTES; B-LYMPHOCYTES; PLASMA CELLS; or NK-cells (KILLER CELLS, NATURAL) depending on the organ or tissues (LYMPHOID TISSUE) to which they migrate.
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