Exploring pathogenic mechanisms of Botrytis cinerea secretome under different ambient pH based on comparative proteomic analysis.
Summary of "Exploring pathogenic mechanisms of Botrytis cinerea secretome under different ambient pH based on comparative proteomic analysis."
Botrytis cinerea causes gray mould rot on over 200 plant species worldwide, resulting in great economic loss every year. Cooperation of proteins secreted by B. cinerea plays an important role in its successful infection to host plants. The ambient pH, as one of the most important environmental parameters, can regulate expression of secreted proteins in various fungal pathogens. In the present study, we mainly investigated the effect of ambient pH on secretome of B. cinerea strain B05.10 with a comparative proteomic method based on 2-DE. Distinct differences in secretome of B. cinerea were found between pH 4 and 6 treatments, and 47 differential spots, corresponding to 21 unique proteins, were identified using MALDI-TOF/TOF. At pH 4, more proteins related to proteolysis were induced, whereas, most of up-accumulated proteins were cell wall degrading enzymes at pH 6. Analysis of gene expression using quantitative real-time PCR suggests that production of most of these proteins was regulated at the level of transcription. These findings indicate that B. cinerea can adjust protein profile of secretome responding to different ambient pH values, and which provide evidence to deeply understand the complicated infecting mechanisms of B. cinerea on a wide range of plant hosts.
Affiliation
Journal Details
This article was published in the following journal.
Name: Journal of proteome research
ISSN: 1535-3907
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22746291
- DOI: http://dx.doi.org/10.1021/pr300365f
Medical and Biotech [MESH] Definitions
Neisseria Cinerea
A species of gram-negative, aerobic bacteria found in the human NASOPHARYNX.
Escherichia Coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Botrytis
A mitosporic Leotiales fungal genus of plant pathogens. It has teleomorphs in the genus Botryotina.
Central Nervous System Agents
A class of drugs producing both physiological and psychological effects through a variety of mechanisms. They can be divided into "specific" agents, e.g., affecting an identifiable molecular mechanism unique to target cells bearing receptors for that agent, and "nonspecific" agents, those producing effects on different target cells and acting by diverse molecular mechanisms. Those with nonspecific mechanisms are generally further classed according to whether they produce behavioral depression or stimulation. Those with specific mechanisms are classed by locus of action or specific therapeutic use. (From Gilman AG, et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p252)
Nosema
A genus of parasitic FUNGI in the family Nosematidae. Some species are pathogenic for invertebrates of economic importance while others are being researched for possible roles in controlling pest INSECTS. They are also pathogenic in humans.
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