Chemical probes for the recognition of cannabinoid receptors in native systems.
Summary of "Chemical probes for the recognition of cannabinoid receptors in native systems."
Receptors made visible: The described biotin-tagged small-molecule probes with excellent affinities for the CB(1) and CB(2) cannabinoid receptors (CB(1) R and CB(2) R) enable direct visualization of these receptors in native cellular systems, including neurons, microglia, and immune cells. This method could overcome some of the limitations of current methodologies and may help to dissect the complexity of the endogenous cannabinoid system.
Affiliation
Department of Organic Chemistry, Universidad Complutense de Madrid, 28040 Madrid (Spain).
Journal Details
This article was published in the following journal.
Name: Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Pages: 6896-9
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22689411
- DOI: http://dx.doi.org/10.1002/anie.201200467
Medical and Biotech [MESH] Definitions
Rna Probes
RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.
Toll-like Receptors
A family of pattern recognition receptors characterized by an extracellular leucine-rich domain and a cytoplasmic domain that share homology with the INTERLEUKIN 1 RECEPTOR and the DROSOPHILA toll protein. Following pathogen recognition, toll-like receptors recruit and activate a variety of SIGNAL TRANSDUCING ADAPTOR PROTEINS.
Receptor, Cannabinoid, Cb2
A subclass of cannabinoid receptor found primarily on immune cells where it may play a role modulating release of CYTOKINES.
Receptor, Cannabinoid, Cb1
A subclass of cannabinoid receptor found primarily on central and peripheral NEURONS where it may play a role modulating NEUROTRANSMITTER release.
Endocannabinoids
Endogenous compounds that bind to CANNABINOID RECEPTORS. They are structurally different from the CANNABINOIDS which are primarily derived from plants.
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