A novel bioassay platform using ferritin-based nanoprobe hydrogel.
Summary of "A novel bioassay platform using ferritin-based nanoprobe hydrogel."
Ferritin-based nanoprobe (FBNP) hydrogel provides solutions to some of the traditional problems of bioassays. The schematic shows 3D and highly specific multiplex assays of markers for AIDS and Sjögren's syndrome using quantum-dot based reporters with different fluorescence emissions for the two syndromes. The advantages of using a hydrogel include that the stability of FBNPs is significantly enhanced within the hydrogel, FBNPs are distributed evenly throughout the hydrogel matrix, and the amount of FBNPs within the hydrogel can be well controlled.
Affiliation
Department of Chemical and Biological Engineering, College of Engineering, Korea University, Anam-Ro 145, Seoul 136-713, Republic of Korea.
Journal Details
This article was published in the following journal.
Name: Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Pages: 4739-44
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22778052
- DOI: http://dx.doi.org/10.1002/adma.201200728
Medical and Biotech [MESH] Definitions
Apoproteins
The protein components of a number of complexes, such as enzymes (APOENZYMES), ferritin (APOFERRITINS), or lipoproteins (APOLIPOPROTEINS).
Hydrogel
A network of cross-linked hydrophilic macromolecules used in biomedical applications.
Chitosan
Deacetylated CHITIN, a linear polysaccharide of deacetylated beta-1,4-D-glucosamine. It is used in HYDROGEL and to treat WOUNDS.
Ferritins
Iron-containing proteins that are widely distributed in animals, plants, and microorganisms. Their major function is to store IRON in a nontoxic bioavailable form. Each ferritin molecule consists of ferric iron in a hollow protein shell (APOFERRITINS) made of 24 subunits of various sequences depending on the species and tissue types.
Biological Assay
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
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