Dielectrophoretic Separation of Prostate Cancer Cells.
Summary of "Dielectrophoretic Separation of Prostate Cancer Cells."
Separation of cancer cells from other biological materials is significant for circulating tumor cell detection in cancer diagnosis and treatment. However, separation of one type of cancer cell from other types of cancer cells can be difficult, since they share similar morphology and biomarkers. In the present work, we have successfully manipulated and isolated LNCaP prostate cancer cells from HCT116 colorectal cancer cells, by dielectrophoresis (DEP) in a microfluidic platform in a continuous operation. In this cell sorter, the prostate cancer cells were treated as target cells and were deflected to a side channel from a main channel as they experienced a negative DEP force, when an AC electric field at the cross-over frequency of the HCT116 cells was supplied. This motion consequently led to the separation of the prostate cancer cells from the colorectal cancer cells. In this manuscript, we report the flow conditions, DEP spectra of the cancer cells and the isolation of LNCaP cells from HCT116 cells. The separation and enrichment factor have been investigated as well.
Affiliation
Dept. of Mechanical Engineering and Biomedical Engineering Program, University of South Carolina, Columbia, SC 29208, USA. guirenwang@sc.edu.
Journal Details
This article was published in the following journal.
Name: Technology in cancer research & treatment
ISSN: 1533-0338
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22775338
- DOI: http://dx.doi.org/
Medical and Biotech [MESH] Definitions
Prostate-specific Antigen
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
Fenretinide
A synthetic retinoid that is used orally as a chemopreventive against prostate cancer and in women at risk of developing contralateral breast cancer. It is also effective as an antineoplastic agent.
Ultrasound, High-intensity Focused, Transrectal
Tissue ablation of the PROSTATE performed by ultrasound from a transducer placed in the RECTUM. The procedure is used to treat prostate cancer (PROSTATIC NEOPLASMS) and benign prostatic hypertrophy (PROSTATIC HYPERPLASIA).
Prostatic Secretory Proteins
Proteins secreted by the prostate gland. The major secretory proteins from the human prostate gland include PROSTATE-SPECIFIC ANTIGEN, prostate-specific acid phosphatase, prostate-specific membrane antigen, and prostate-specific protein-94.
Prostatic Neoplasms
Tumors or cancer of the PROSTATE.
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