Optofluidic integrated cell sorter fabricated by femtosecond lasers.
Summary of "Optofluidic integrated cell sorter fabricated by femtosecond lasers."
The main trend in optofluidics is currently towards full integration of the devices, thus improving automation, compactness and portability. In this respect femtosecond laser microfabrication is a very powerful technology given its capability of producing both optical waveguides and microfluidic channels. The current challenge in biology is the possibility to perform bioassays at the single cell level to unravel the hidden complexity in nominally homogeneous populations. Here we report on a new device implementing a fully integrated fluorescence-activated cell sorter. This non-invasive device is specifically designed to operate with a limited amount of cells but with a very high selectivity in the sorting process. Characterization of the device with beads and validation with human cells are presented.
Istituto di Fotonica e Nanotecnologie (IFN) - CNR, Dipartimento di Fisica - Politecnico di Milano, P.zza Leonardo da Vinci 32, 20133 Milano, Italy. firstname.lastname@example.org.
This article was published in the following journal.
Name: Lab on a chip
Medical and Biotech [MESH] Definitions
Tunable liquid lasers with organic compounds (i.e., dye) which have a strong absorption band, used as the active medium. During emission, the dye has to be optically excited by another light source (e.g., another laser or flash lamp). The range of the emission wavelength may be anywhere from the ultraviolet to the near infrared (i.e., from 180 to 1100nm). These lasers are operated in continuous wave and pulsed modes. (UMDNS, 2005)
Gas lasers with excited dimers (i.e., excimers) as the active medium. The most commonly used are rare gas monohalides (e.g., argon fluoride, xenon chloride). Their principal emission wavelengths are in the ultraviolet range and depend on the monohalide used (e.g., 193 nm for ArF, 308 nm for Xe Cl). These lasers are operated in pulsed and Q-switched modes and used in photoablative decomposition involving actual removal of tissue. (UMDNS, 2005)
Lasers in which a gas lasing medium is stimulated to emit light by an electric current or high-frequency oscillator.
Lasers which use a solid, as opposed to a liquid or gas, as the lasing medium. Common materials used are crystals, such as YAG (YTTRIUM aluminum garnet); alexandrite; and CORUNDUM, doped with a rare earth element such as a NEODYMIUM; ERBIUM; or HOLMIUM. The output is sometimes additionally modified by addition of non-linear optical materials such as potassium titanyl phosphate crystal, which for example is used with neodymium YAG lasers to convert the output light to the visible range.
Lasers with a semiconductor diode as the active medium. Diode lasers transform electric energy to light using the same principle as a light-emitting diode (LED), but with internal reflection capability, thus forming a resonator where a stimulated light can reflect back and forth, allowing only a certain wavelength to be emitted. The emission of a given device is determined by the active compound used (e.g., gallium arsenide crystals doped with aluminum or indium). Typical wavelengths are 810, 1,060 and 1,300 nm. (From UMDNS, 2005)
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