Rapid frozen sections in neuropathology.
Summary of "Rapid frozen sections in neuropathology."
Neuropathological evaluation of frozen sections requires a) special expertise in neuropathological specimen assessment and neurooncology as well as b) a trustful and open communication culture with the neurosurgeons. In addition to frozen sections, cytological examinations of smear and touch preparations as supporting methods are available to reach a correct diagnosis: these additional methods should therefore be performed whenever possible. Besides evaluation of biopsy specimens, appraisal of resection specimens and resection margin controls are of high clinical relevance. In the case of diffusely infiltrating central nervous system (CNS) neoplasms, in particular gliomas, resection margin control is often not feasible in contrast to other types of solid tumor.
Affiliation
Abteilung für Neuropathologie, Institut für Pathologie, Medizinische Universität Graz, Auenbruggerplatz 25, 8036, Graz, Österreich, johannes.haybaeck@medunigraz.at.
Journal Details
This article was published in the following journal.
Name: Der Pathologe
ISSN: 1432-1963
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22868403
- DOI: http://dx.doi.org/10.1007/s00292-012-1604-x
Medical and Biotech [MESH] Definitions
Frozen Sections
Thinly cut sections of frozen tissue specimens prepared with a cryostat or freezing microtome.
Cryoultramicrotomy
The technique of using a cryostat or freezing microtome, in which the temperature is regulated to -20 degrees Celsius, to cut ultrathin frozen sections for microscopic (usually, electron microscopic) examination.
Cryoelectron Microscopy
Electron microscopy involving rapid freezing of the samples. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.
Ice Cream
A frozen dairy food made from cream or butterfat, milk, sugar, and flavorings. Frozen custard and French-type ice creams also contain eggs.
Microtomy
The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.
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