Selective in situ functionalization of biosensors on LOC devices using laminar co-flow.
Summary of "Selective in situ functionalization of biosensors on LOC devices using laminar co-flow."
Many applications involving lab-on-a-chip (LOC) devices are prevented from entering the market because of difficulties to achieve mass production and impart suitable properties allowing long-term storage. To integrate biosensors on these microfluidic chips, one of the main restrictions is the fabrication and stability of the molecular modifications that must be performed on the surfaces of the sensors for a given application. The complexity of the problem increases exponentially when the LOC integrates several of these sensors. Here we present a system based on laminar co-flow to perform an on-chip selective surface bio-functionalization of LOC-integrated sensors. This method has the advantage that the surface modification protocols are performed in situ before analyte detection. This approach reduces the burdens during LOC fabrication, keeping the required reagents stored outside of the detection structure in suitable wet conditions. The proof of concept is demonstrated through an optical characterization followed by electronic detection based on a novel differential impedance measurement setup. The system can be easily scaled to incorporate several sensors with distinct biosensing targets in a single chip.
Affiliation
Nanobioengineering group, Institute for Bioengineering of Catalonia (IBEC), Baldiri Reixac 10-12, 08028, Barcelona, Spain. caparra@ibec.pcb.ub.es.
Journal Details
This article was published in the following journal.
Name: Lab on a chip
ISSN: 1473-0189
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22868270
- DOI: http://dx.doi.org/10.1039/c2lc40107j
Medical and Biotech [MESH] Definitions
Pulsatile Flow
Rhythmic, intermittent propagation of a fluid through a BLOOD VESSEL or piping system, in contrast to constant, smooth propagation, which produces laminar flow.
Primed In Situ Labeling
A technique that labels specific sequences in whole chromosomes by in situ DNA chain elongation or PCR (polymerase chain reaction).
Spectral Karyotyping
The simultaneous identification of all chromosomes from a cell by fluorescence in situ hybridization (IN SITU HYBRIDIZATION, FLUORESCENCE) with chromosome-specific florescent probes that are discerned by their different emission spectra.
In Situ Hybridization, Fluorescence
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
Oxygenators, Membrane
Devices in which blood and oxygen are separated by a semipermeable membrane, generally of Teflon or polypropylene, across which gas exchange occurs. The membrane may be arranged as a series of parallel plates or as a number of hollow fibers; in the latter arrangement, the blood may flow inside the fibers, which are surrounded by gas, or the blood may flow outside the fibers and the gas inside the fibers. (Dorland, 28th ed)
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