Primary microRNA precursor transcripts are localized at postsynaptic densities in adult mouse forebrain.
Summary of "Primary microRNA precursor transcripts are localized at postsynaptic densities in adult mouse forebrain."
In a previous study, we reported that microRNA (miRNA) precursors are expressed in synaptic fractions within adult mouse forebrain, where they are enriched at postsynaptic densities (PSDs). However, because that study employed qRT-PCR primers that recognize the hairpin region, it was not able to distinguish between primary microRNA gene transcripts (pri-miRs) and small hairpin precursors (pre-miRs). Here, using primer sets that selectively measure regions upstream, downstream and flanking the hairpin, we demonstrate that pri-miRs are present in synaptic fractions (enriched several-fold relative to total tissue homogenate) and are especially enriched in isolated PSDs. Drosha and DGCR8 proteins are also expressed in synaptic fractions and PSDs, and are tightly associated with pri-miRs as assessed by co-immunoprecipitation under stringent conditions. Pri-miRs, drosha, and DGCR8 are highly enriched in fractions that contain mRNA transport particles, and cytosolic drosha is associated with kinesin heavy chain; these findings suggest that pri-miRs are transported to synaptic regions in a manner similar to mRNAs. The present study supports the notion that miRNA biogenesis occurs locally near synapses in a regulated fashion. © 2012 The Authors Journal of Neurochemistry © 2012 International Society for Neurochemistry.
Department of Psychiatry and Psychiatric Institute, University of Illinois at Chicago, Chicago, IL, USA; Graduate Program in Neuroscience, University of Illinois at Chicago, Chicago, IL, USA.
This article was published in the following journal.
Name: Journal of neurochemistry
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22897173
- DOI: http://dx.doi.org/10.1111/j.1471-4159.2012.07921.x
Medical and Biotech [MESH] Definitions
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