Advertisement

Topics

Nanoparticle-modified monolithic pipette tips for phosphopeptide enrichment.

Summary of "Nanoparticle-modified monolithic pipette tips for phosphopeptide enrichment."

We have developed nanoparticle-modified monoliths in pipette tips for selective and efficient enrichment of phosphopeptides. The 5 μL monolithic beds were prepared by UV-initiated polymerization in 200 μL polypropylene pipette tips and either iron oxide or hydroxyapatite nanoparticles were used for monolith modification. Iron oxide nanoparticles were prepared by a co-precipitation method and stabilized by citrate ions. A stable coating of iron oxide nanoparticles on the pore surface of the monolith was obtained via multivalent electrostatic interactions of citrate ions on the surface of nanoparticles with a quaternary amine functionalized poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) monolith. Hydroxyapatite nanoparticles were incorporated into the poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) monolith by simply admixing them in the polymerization mixture followed by in situ polymerization. The nanoparticle-modified monoliths were compared with commercially available titanium dioxide pipette tips. Performance of the developed and commercially available sorbents was demonstrated with the efficient and selective enrichment of phosphopeptides from peptide mixtures of α-casein and β-casein digests followed by off-line MALDI/MS analysis.

Affiliation

Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic, v. v. i, 602 00, Brno, Czech Republic, krenkova@iach.cz.

Journal Details

This article was published in the following journal.

Name: Analytical and bioanalytical chemistry
ISSN: 1618-2650
Pages:

Links

DeepDyve research library

PubMed Articles [5437 Associated PubMed Articles listed on BioPortfolio]

Conductive polycrystalline diamond probes for local anodic oxidation lithography.

This is the first report characterizing local anodic oxidation (LAO) lithography performed using conductive monolithic polycrystalline diamond (MD) and conductive polycrystalline diamond-coated (DC) t...

Three-dimensional ordered titanium dioxide-zirconium dioxide film-based microfluidic device for efficient on-chip phosphopeptide enrichment.

Microfluidic technology plays a significant role in separating biomolecules, because of its miniaturization, integration, and automation. Introducing micro/nanostructured functional materials can impr...

Failure Probability of Three Designs of Zirconia Crowns.

This study used a two-parameter Weibull analysis for evaluation of the lifespan of fully or partially porcelain-/glaze-veneered zirconia crowns after fatigue test. A sample of 60 first molars were sel...

Immobilized metal affinity chromatography optimized for the analysis of extracellular phosphorylation.

Phosphorylation is the most widely studied post-translational modification. Its role within the cell has been the focus of numerous large scale studies. Recently there is growing evidence on the biolo...

Aptamer functionalized hydrophilic polymer monolith with gold nanoparticles modification for the sensitive detection of human α-thrombin.

Low abundant proteins of body fluids participate nearly all physiological processes and indicate various kinds of diseases. The development of specific enrichment techniques is the key to identify and...

Clinical Trials [859 Associated Clinical Trials listed on BioPortfolio]

Rifaximin vs Placebo for the Prevention of Encephalopathy in Patients Treated by TIPS

TIPS has been used for 20 years, as a means of reducing portal pressure in patients with cirrhosis and portal hypertension related complications. TIPS proved more effective than alternativ...

Trauma-informed Personalized Scripts (TIPS) for Relationship Abuse

This pilot study will evaluate a computer-based intervention titled "Trauma-informed Personalized Scripts" (TIPS). The goal of this intervention is to improve patient-provider communicatio...

TIPS With Coated Stents for Refractory Ascites in Patients With Cirrhosis

The main end point of the study is to assess survival in cirrhotic patients with refractory ascites treated by TIPS (using PTFE covered stents) vs patients treated by paracentesis + albumi...

Marginal Fit of Porcelain Veneers After Finishing Prepared Surfaces With Ultrasonic Tips

The aim of the study is to assess the marginal fit following preparation finishing with ultrasonic tips of porcelain veneers compared to the ordinary preparation using burs only. Two-hundr...

Feasibility of Critical Flicker Frequency Procedure for the Diagnosis of Minimal Encephalopathy

All patients eligible for TIPS (Transjugular intrahepatic portosystemic shunt) procedure will be considered for inclusion. After written inform consent, psychometric tests in order to calc...

Medical and Biotech [MESH] Definitions

Food derived from genetically modified organisms (ORGANISMS, GENETICALLY MODIFIED).

New immature growth of a plant including stem, leaves, tips of branches, and SEEDLINGS.

A modified nucleoside which is present in the first position of the anticodon of tRNA-tyrosine, tRNA-histidine, tRNA-asparagine and tRNA-aspartic acid of many organisms. It is believed to play a role in the regulatory function of tRNA. Nucleoside Q can be further modified to nucleoside Q*, which has a mannose or galactose moiety linked to position 4 of its cyclopentenediol moiety.

A non-medical term defined by the lay public as a food that has little or no preservatives, which has not undergone major processing, enrichment or refinement and which may be grown without pesticides. Health foods have been attributed with the ability to prevent the development of diseases, slow the aging process, and prolong life. (from Segen, The Dictionary of Modern Medicine, 1992)

The enrichment of a terrestrial or aquatic ECOSYSTEM by the addition of nutrients, especially nitrogen and phosphorus, that results in a superabundant growth of plants, ALGAE, or other primary producers. It can be a natural process or result from human activity such as agriculture runoff or sewage pollution. In aquatic ecosystems, an increase in the algae population is termed an algal bloom.

Quick Search
Advertisement
 


DeepDyve research library

Searches Linking to this Article