Prospectively Isolated Cancer-Associated CD10 Fibroblasts Have Stronger Interactions with CD133 Colon Cancer Cells than with CD133 Cancer Cells.
Summary of "Prospectively Isolated Cancer-Associated CD10 Fibroblasts Have Stronger Interactions with CD133 Colon Cancer Cells than with CD133 Cancer Cells."
Although CD133 has been reported to be a promising colon cancer stem cell marker, the biological functions of CD133(+) colon cancer cells remain controversial. In the present study, we investigated the biological differences between CD133(+) and CD133(-) colon cancer cells, with a particular focus on their interactions with cancer-associated fibroblasts, especially CD10(+) fibroblasts. We used 19 primary colon cancer tissues, 30 primary cultures of fibroblasts derived from colon cancer tissues and 6 colon cancer cell lines. We isolated CD133(+) and CD133(-) subpopulations from the colon cancer tissues and cultured cells. In vitro analyses revealed that the two populations showed similar biological behaviors in their proliferation and chemosensitivity. In vivo analyses revealed that CD133(+) cells showed significantly greater tumor growth than CD133(-) cells (P = 0.007). Moreover, in cocultures with primary fibroblasts derived from colon cancer tissues, CD133(+) cells exhibited significantly more invasive behaviors than CD133(-) cells (P<0.001), especially in cocultures with CD10(+) fibroblasts (P<0.0001). Further in vivo analyses revealed that CD10(+) fibroblasts enhanced the tumor growth of CD133(+) cells significantly more than CD10(-) fibroblasts (P<0.05). These data demonstrate that the in vitro invasive properties and in vivo tumor growth of CD133(+) colon cancer cells are enhanced in the presence of specific cancer-associated fibroblasts, CD10(+) fibroblasts, suggesting that the interactions between these specific cell populations have important roles in cancer progression. Therefore, these specific interactions may be promising targets for new colon cancer therapies.
Affiliation
Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Journal Details
This article was published in the following journal.
Name: PloS one
ISSN: 1932-6203
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20711432
- DOI: http://dx.doi.org/10.1371/journal.pone.0012121
Medical and Biotech [MESH] Definitions
Seer Program
A cancer registry mandated under the National Cancer Act of 1971 to operate and maintain a population-based cancer reporting system, reporting periodically estimates of cancer incidence and mortality in the United States. The Surveillance, Epidemiology, and End Results (SEER) Program is a continuing project of the National Cancer Institute of the National Institutes of Health. Among its goals, in addition to assembling and reporting cancer statistics, are the monitoring of annual cancer incident trends and the promoting of studies designed to identify factors amenable to cancer control interventions. (From National Cancer Institute, NIH Publication No. 91-3074, October 1990)
Fibromatosis, Abdominal
A relatively large mass of unusually firm scarlike connective tissue resulting from active participation of fibroblasts, occurring most frequently in the abdominal muscles of women who have borne children. The fibroblasts infiltrate surrounding muscle and fascia. (Stedman, 25th ed)
Mucinoses
Mucoid states characterized by the elevated deposition and accumulation of mucin (mucopolysaccharides) in dermal tissue. The fibroblasts are responsible for the production of acid mucopolysaccharides (GLYCOSAMINOGLYCANS) in the ground substance of the connective tissue system. When fibroblasts produce abnormally large quantities of mucopolysaccharides as hyaluronic acid, chondroitin sulfate, or heparin, they accumulate in large amounts in the dermis.
Chemotherapy, Cancer, Regional Perfusion
Neoplasm drug therapy involving an extracorporeal circuit with temporary exclusion of the tumor-bearing area from the general circulation during which high concentrations of the drug are perfused to the isolated part.
Retroviruses, Simian
Classes of retroviruses for which monkeys or apes are hosts. Those isolated from the West African green monkey and the Asian rhesus macaque monkey are of particular interest because of their similarities to viruses causing cancer and acquired immunodeficiency syndrome (AIDS) in humans.
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