Advertise here Publish your press releases here Sponsor BioPortfolio
Follow us on Twitter Sign up for daily news and research emails Contributors wanted

Validation of a Micellar Liquid Chromatographic Method for Determination of Caffeine and Non-Steroidal Anti-Inflammatories.

04:07 EDT 18th April 2014 | BioPortfolio

Summary of "Validation of a Micellar Liquid Chromatographic Method for Determination of Caffeine and Non-Steroidal Anti-Inflammatories."

An accurate, simple, sensitive and selective micellar liquid chromatographic method has been developed for the simultaneous determination of caffeine (CAF) and two non-steroidal anti-inflammatory drugs, namely ibuprofen (IBU) and ketoprofen (KET). The chemometric approach was applied to the optimization of separation of the studied drugs. To optimize their separation, the effect of six experimental parameters on retention was investigated by means of multivariate analysis. Separation was conducted using an ODS C18 (150 × 4.6 mm i.d.) stainless steel column at ambient temperature with UV detection at 260 nm. A mobile phase composed of 40 mM sodium dodecyl sulphate (SDS), 10% 1-propanol and 0.3% tri-ethylamine in 0.02 M phosphoric acid adjusted to pH 3 has been used at a flow rate of 1 mL/min. Regression models were characterized by both descriptive and predictive ability (R(2) ≥ 97.9% and Rcv(2) ≥ 96.2%) and allowed the chromatographic separation of the drugs with a good resolution and a total analysis time within 15 min. The calibration curves were rectilinear over the concentration ranges of 2.0-25.0, 1.5-15.0 and 1.0-10.0 µg/mL for IBU, KET and CAF, respectively, with detection limits of 1.2, 1.0 and 0.6 µg/mL, and quantification limits of 1.6, 1.2 and 0.8 µg/mL, respectively. The results obtained were in good agreement with those obtained by the comparison method.


1Department of Medicinal Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.

Journal Details

This article was published in the following journal.

Name: Journal of chromatographic science
ISSN: 1945-239X


PubMed Articles [9519 Associated PubMed Articles listed on BioPortfolio]

HPLC Method with Solid-Phase Extraction for Determination of (R)- and (S)-Ketoprofen in Plasma without Caffeine Interference: Application to Pharmacokinetic Studies in Rats.

A fast and reproducible high-performance liquid chromatography method has been developed for the determination of (R)- and (S)-ketoprofen. Ketoprofen enantiomers were determined in plasma samples (50...

Validated liquid chromatographic determination of a novel ACE inhibitor in the presence of its hydrolytic and oxidative degradation products as per ICH guidelines.

Imidapril hydrochloride (IMD) is a recently developed prodrug-type angiotensin-converting enzyme (ACE) inhibitor. Due to its instability under both hydrolytic and oxidative conditions, development of...

Development and Validation of an HPLC-DAD Analysis for Pharmacopoeial Qualification of Industrial Capsicum Extracts.

A reverse-phase high-performance liquid chromatography method was developed to quantify capsaicin (trans-8-methyl-N-vanillyl-6-nonenamid), dihydrocapsaicin (8-methyl-N-vanillylnonanamide) and the main...

High-performance liquid chromatographic determination of rivastigmine in human plasma for application in pharmacokinetic studies.

A simple and reproducible HPLC method with spectrophotometric detection was developed for determination of rivastigmine in human plasma. Liquid-liquid extraction of rivastigmine and donepezil (as inte...

Determination of dicyandiamide in dairy products by high performance liquid chromatography.

A high performance liquid chromatographic (HPLC) method for the determination of dicyandiamide in dairy products was developed. The sample was extracted by acetonitrile, separated on an XBridge Amide...

Clinical Trials [1611 Associated Clinical Trials listed on BioPortfolio]

Effects of Low Doses of Caffeine on Mood, Physiology and Mental Function

It is often assumed that levels of caffeine found in ‘decaffeinated’ beverages are below any psychopharmacological threshold. However, recent findings indicate that caffeine doses as l...

The Effect of Caffeine in Elderly Citizen Following Eight Hour Abstinence From Caffeine Containing Drinks & Foods

The study investigated the effect of caffeine on physical performance in healthy citizens aged over 70 years following eight hours of abstinence from caffeine containing drinks and foods....

Caffeine for Excessive Daytime Somnolence in Parkinson's Disease

Many patients with Parkinson's disease (PD) have sleep problems, including excessive sleepiness during the day. This is probably due to degeneration of sleep-regulating areas in the brain...

The Effect of Caffeine in Elderly Citizens

The study investigated the effect of caffeine on physical performance in healthy citizens aged over 70 years. The main hypothesis was that 6 mg/kg caffeine would improve cycling endurance...

Effects of Caffeine in Tinnitus

Caffeine intake is reported by many authors to enhance the perception of tinnitus. The aim of this study is to determine the effects of 1 month caffeine intake reduction in the scores of v...

Medical and Biotech [MESH] Definitions

A hybrid separation technique combining both chromatographic and electrophoretic separation principles. While the method was invented to separate neutral species, it can also be applied to charged molecules such as small peptides.

Chromatographic techniques in which the mobile phase is a liquid.

Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.

Validation of the SEX of an individual by inspection of the GONADS and/or by genetic tests.

Validation of the SEX of an individual by inspection of the GONADS and/or by genetic tests.

Search BioPortfolio:

Searches Linking to this Article