Animal model of radiogenic bone damage to study mandibular osteoradionecrosis.
Summary of "Animal model of radiogenic bone damage to study mandibular osteoradionecrosis."
OBJECTIVE:
The objective of the study was to create an animal model to study mandibular osteoradionecrosis (ORN) using high-dose rate (HDR) brachytherapy.
METHODS:
Ten Sprague-Dawley male rats were used in this study. Six rats received a single dose of 30 Gy using an HDR remote afterloading machine via a brachytherapy catheter placed along the left hemimandible. The remaining 4 rats served as controls with catheter placement without radiation (sham). On the day following irradiation or sham, all 3 left mandibular molars were atraumatically extracted. Twenty-eight days after irradiation, mandibles were examined using nondecalcified histology with sequential fluorochrome labeling, decalcified histology, and micro-computed tomography scanning.
RESULTS:
Irradiated rats demonstrated exposed bone at the extraction sockets, whereas the control animals had complete mucosalization. Alopecia was also seen in the irradiated group. Both histologic and radiologic analyses of the mandible specimens demonstrated a reduction in bone formation in the radiated mandibles as compared with controls.
CONCLUSIONS:
Our HDR brachytherapy model incorporating postradiation dental extractions has successfully demonstrated reproducible radiogenic mandibular bone damage analogous to the clinical ORN. Although clinical criteria continue to be used today in describing ORN, this model can serve as a platform for future studies to define ORN and delineate its pathogenesis.
Affiliation
Division of Head and Neck Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
Journal Details
This article was published in the following journal.
Name: American journal of otolaryngology
ISSN: 1532-818X
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20719407
- DOI: http://dx.doi.org/10.1016/j.amjoto.2010.06.001
Medical and Biotech [MESH] Definitions
Osteoradionecrosis
Necrosis of bone following radiation injury.
Denture, Complete, Lower
A complete denture replacing all the natural mandibular teeth and associated structures. It is completely supported by the oral tissue and underlying mandibular bone.
Models, Statistical
Statistical formulations or analyses which, when applied to data and found to fit the data, are then used to verify the assumptions and parameters used in the analysis. Examples of statistical models are the linear model, binomial model, polynomial model, two-parameter model, etc.
Phalloidine
Very toxic polypeptide isolated mainly from AMANITA phalloides (Agaricaceae) or death cup; causes fatal liver, kidney and CNS damage in mushroom poisoning; used in the study of liver damage.
1-naphthylisothiocyanate
A tool for the study of liver damage which causes bile stasis and hyperbilirubinemia acutely and bile duct hyperplasia and biliary cirrhosis chronically, with changes in hepatocyte function. It may cause skin and kidney damage.
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