Advertisement

Topics

Two female-specific DSX proteins are encoded by the sex-specific transcripts of dsx, and are required for female sexual differentiation in two wild silkmoth species, Antheraea assama and Antheraea mylitta (Lepidoptera, Saturniidae).

Summary of "Two female-specific DSX proteins are encoded by the sex-specific transcripts of dsx, and are required for female sexual differentiation in two wild silkmoth species, Antheraea assama and Antheraea mylitta (Lepidoptera, Saturniidae)."

Doublesex (dsx) is the bottom most gene of the sex determination cascade of Drosophila melanogaster. The pre-mRNA of dsx splices to produce male- and female-specific transcripts which code for the male- and female-specific proteins, respectively. dsx homologues have been characterized from different (many in Diptera, two in Hypmenoptera and only one in Lepidoptera) insect species. Sex-specific splice forms of dsx pre-mRNA in all these species code for one male and one female-specific DSX proteins, which regulate the downstream target genes responsible for sex-specific characters. In the present study we have cloned and characterized the dsx homologues from two saturniid silkmoths, Antheraea assama and A. mylitta. The divergence time between Saturniidae and Bombycidae to which the domesticated silkworm, B. mori belongs is estimated to be around 160.9 MY. Interestingly, the dsx pre-mRNA of these wild silkmoths sex-specifically splices to generate multiple splice variants. On the basis of their open reading frame (ORF) and conceptual translation, two female-specific (DSX(F1) and DSX(F2)) and one male- specific (DSX(M)) proteins could be inferred, in both the moths. Presence or absence of a 15bp stretch within the ORF of the two groups of female-specific transcripts resulted in the production of two distinct female-specific DSX proteins. The sex-specific DSX proteins have common amino-terminal sequence but sex-specific carboxy termini. The two female-specific DSX proteins (DSX(F1) and DSX(F2)) share common DNA binding domain (DM domain) and oligomerisation domain (OD domain) and differ only at their extreme C-termini by 21aa. Functional analysis of dsx transcripts in A. assama by dsRNA mediated knock-down resulted in complete abolition of expression of vitellogenin and hexamerin genes, the direct targets of the DSX proteins, irregular differentiation of gonads, and drastic reduction in fecundity and hatchability. Together, these results suggest the involvement of both the female-specific DSX proteins in the process of female sexual differentiation. Further, conservation of the 4th exon sequence, especially the PESS sequence responsible for the sex-specific splicing of Bmdsx in the female-specific transcripts of Aadsx and Amydsx, indicated the existence of a common mechanism of sex-specific splicing of dsx homologues in silkmoths. To our knowledge this is the first report of existence of multiple splice forms of dsx pre-mRNA encoding two female-specific DSX proteins.

Affiliation

Centre of Excellence for Genetics and Genomics of Silkmoths, Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, Tuljaguda, Nampally, Hyderabad 500001, India.

Journal Details

This article was published in the following journal.

Name: Insect biochemistry and molecular biology
ISSN: 1879-0240
Pages:

Links

DeepDyve research library

PubMed Articles [29284 Associated PubMed Articles listed on BioPortfolio]

The Effect of Timing of Female Vibrational Reply on Male Signalling and Searching Behaviour in the Leafhopper Aphrodes makarovi.

Sexual communication in animals often involves duetting characterized by a coordinated reciprocal exchange of acoustic signals. We used playback experiments to study the role of timing of a female rep...

Faces on Her and His Mind: Female and Likable.

Faces are a valuable source of non-verbal information for daily life social interaction. Mounting evidence points to gender specificity in face perception. Here we search for the factors that can pote...

CD8+ T Cells Mediate Female-Dominant IL-4 Production and Airway Inflammation in Allergic Asthma.

The prevalence and severity of bronchial asthma are higher in females than in males after puberty. Although antigen-specific CD8+ T cells play an important role in the development of asthma through th...

Transcriptional Truncation of the Long Coding Imprinted Gene Usp29.

Usp29 (Ubiquitin-specific protease 29) is a paternally expressed gene located upstream of another imprinted gene Peg3. In the current study, the transcription of this long coding gene spanning a 250-k...

Navigating the thin-ideal in an athletic world: influence of coach communication on female athletes' body image and health choices.

This study sought to investigate how interpersonal communication between coaches and female athletes influences the female athletes' perceptions of body image and health choices. Much of the current l...

Clinical Trials [6323 Associated Clinical Trials listed on BioPortfolio]

Effects of Spinal Cord Injury on Female Sexual Response

The long term aims of this project are to 1) Understand the effect of varying degrees and levels of SCI and dysfunction(SCI/D)on females sexual response: 2) Identify the specific neuronal ...

Investigation of Gender Specificity of the Effects of Furosemide in Healthy Female and Male Volunteers

In this study the gender specificity of the effects of furosemide in female and male volunteers will be investigated. The healthy volunteers receive 1. furosemide and 2. aminohippurate sod...

An Open-label, Multi-center Study Evaluating the Validity, Reliability, and Responsiveness of a New Female-specific Angina Questionnaire in Women With Chronic Angina Treated With Ranolazine Extended-release Tablets (CVT 3041)

Of the estimated 8.9 million angina patients in the United States, over half are women (4.6 million). Per ACC/AHA guidelines, the goal of antianginal therapy is the complete or near compl...

Education Program to Promote Female Condom Use

The female condom offers women a potentially important option for HIV prevention. This study will develop and evaluate a health education program to increase female condom use among ethni...

Sexually Dimorphic Effects of GHRH in Adult Growth Hormone Testing

Specific Aim 1 Healthy male and female subjects and growth hormone (GH) deficient subjects display sexually dimorphic GH responses to GHRH administration Specific Aim 2 GH responses to GH...

Medical and Biotech [MESH] Definitions

MYCOBACTERIUM infections of the female reproductive tract (GENITALIA, FEMALE).

Tumor or cancer of the female reproductive tract (GENITALIA, FEMALE).

Pathological processes involving the female reproductive tract (GENITALIA, FEMALE).

Pathological processes of the female URINARY TRACT and the reproductive system (GENITALIA, FEMALE).

Chemical substances or agents with contraceptive activity in females. Use for female contraceptive agents in general or for which there is no specific heading.

Quick Search
Advertisement
 


DeepDyve research library

Relevant Topics

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. During DNA sequencing, the bases of a small fragment of DNA are sequentially identified from signals emitted as each fragment is re-synthesized from a ...


Searches Linking to this Article