SCAR Markers: A potential tool for Authentication of Herbal Drugs.
Summary of "SCAR Markers: A potential tool for Authentication of Herbal Drugs."
Randomly Amplified Polymorphic DNA (RAPD) is easy to develop and simple molecular marker, but lack of reproducibility makes it less reliable for authentication of herbal drugs. Besides RAPD, other popular PCR and non-PCR based markers like AFLP, ISSR, SSR and RFLP are also used for authentication. However, these also have disadvantages like use of radioactive isotopes, high cost and absolute requirement of sequence information. Therefore, it is a better option to improve the reproducibility of RAPD by converting RAPD amplicons into Sequence Characterized Amplified Region (SCAR) markers. SCAR markers are easy, reliable and reproducible thus, have an advantage over RAPD markers for authentication of medicinal herbs used in the preparation of traditional medicines. These markers however, have been developed for only a few medicinal herbs. This review is an attempt to evaluate critically the role of SCAR markers in authentication of medicinal herbs used in traditional formulations.
Affiliation
Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi-110062, India.
Journal Details
This article was published in the following journal.
Name: Fitoterapia
ISSN: 1873-6971
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20723584
- DOI: http://dx.doi.org/10.1016/j.fitote.2010.08.002
Medical and Biotech [MESH] Definitions
Drugs, Chinese Herbal
Chinese herbal or plant extracts which are used as drugs to treat diseases or promote general well-being. The concept does not include synthesized compounds manufactured in China.
Polygonum
A plant genus of the family POLYGONACEAE that is an ingredient of Shou-Wu-Pian, a Chinese herbal preparation (DRUGS, CHINESE HERBAL). The common name of black bindweed also refers to TAMUS or Fallopia (use POLYGONACEAE).
Keloid
A sharply elevated, irregularly shaped, progressively enlarging scar resulting from formation of excessive amounts of collagen in the dermis during connective tissue repair. It is differentiated from a hypertrophic scar (CICATRIX, HYPERTROPHIC) in that the former does not spread to surrounding tissues.
Coix
A plant genus of the family POACEAE. The seed is used in folk medicine (DRUGS, CHINESE HERBAL).
Perilla Frutescens
A plant genus of the family LAMIACEAE that is an ingredient of Banxia Houpu (DRUGS, CHINESE HERBAL).
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