Microwave-assisted extraction of human hair proteins.

19:11 EDT 1st July 2015 | BioPortfolio

Summary of "Microwave-assisted extraction of human hair proteins."

The effectiveness of microwave-assisted extraction of proteins from human hair samples was evaluated. Extractions were performed from 2-mg hair samples in an extraction solution consisting of 25mM Tris-HCl (pH8.5), 2.6M thiourea, 5M urea, and 5% mercaptoethanol. During extraction, samples were exposed to microwave radiation (600W) for a specified incubation period (5-120min). The extraction efficiency of samples that had been incubated for 60min was similar to that of samples that had been heated at 50 degrees C for 24h using the conventional Shindai method.

Affiliation

Department of Chemistry, Chungnam National University, Daejeon, 305-764, South Korea.

Journal Details

This article was published in the following journal.

Name: Analytical biochemistry
ISSN: 1096-0309
Pages:

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