Microwave-assisted extraction of human hair proteins.

19:49 EDT 2nd September 2015 | BioPortfolio

Summary of "Microwave-assisted extraction of human hair proteins."

The effectiveness of microwave-assisted extraction of proteins from human hair samples was evaluated. Extractions were performed from 2-mg hair samples in an extraction solution consisting of 25mM Tris-HCl (pH8.5), 2.6M thiourea, 5M urea, and 5% mercaptoethanol. During extraction, samples were exposed to microwave radiation (600W) for a specified incubation period (5-120min). The extraction efficiency of samples that had been incubated for 60min was similar to that of samples that had been heated at 50 degrees C for 24h using the conventional Shindai method.

Affiliation

Department of Chemistry, Chungnam National University, Daejeon, 305-764, South Korea.

Journal Details

This article was published in the following journal.

Name: Analytical biochemistry
ISSN: 1096-0309
Pages:

Links

PubMed Articles [20569 Associated PubMed Articles listed on BioPortfolio]

A quick method for determination of psychoactive agents in serum and hair by using capillary electrophoresis and mass spectrometry.

The aim of the research was to develop a new sensitive method for simultaneously the determination of psychoactive drugs: 1-benzylpiperazine, 7-aminoclonazepam, alprazolam, clonazepam, diazepam, estaz...

Dynamic microwave assisted extraction coupled with dispersive micro-solid-phase extraction of herbicides in soybeans.

Non-polar solvent dynamic microwave assisted extraction was firstly applied to the treatment of high-fat soybean samples. In the dispersive micro-solid-phase extraction (d-µ-SPE), the herbicides in t...

Microwave-assisted digestion combined with silica-based spin column for DNA isolation from human bones.

A protocol for the extraction of DNA from ancient skeletal material was developed. Bone specimen samples powder or slice, buffer, pretreatment and extraction methodologies were compared in order to in...

Antioxidant Compounds from Vegetable Matrices: Biosynthesis, Occurrence, and Extraction Systems.

Natural antioxidants such as vitamin C, tocopherols and tocotrienols, carotenoids, and phenolic compounds are largely distributed in plant products. Most of them are not synthesized by human and need ...

Accurate analysis and evaluation of acidic plant growth regulators in transgenic and non-transgenic edible oils with facile microwave-assisted extraction-derivatization.

Determination of plant growth regulators (PGRs) in signal transduction system (STS) is significant for transgenic food safety, but may be challenged by poor accuracy and analytes instability. In this ...

Clinical Trials [2677 Associated Clinical Trials listed on BioPortfolio]

An Open Label, Canadian Phase IIIb Study With Ovidrel in Ovulation Induction (OI) and Assisted Reproductive Technique (ART)

With the development of the recombinant gonadotrophins, the use of human source proteins for the production of hormones has become unnecessary. These proteins, which are found in urinary p...

Comparative Study of Microwave Radiometry and Ultrasonography for the Diagnosis of Acute Appendicitis

This study will evaluate the use of microwave radiometry in a population of patients diagnosed with acute appendicitis and treated with appendectomy. The main purpose of the study is to re...

Behaviors of Children Ages 15 Months to Five Years Around Microwave Ovens

The purpose of this study is to assess the typical behavior of children ages 15 months to five years around a microwave oven. The UCH Burn and Complex Wound Center has observed scald burn ...

The Effect of Laser Hair Removal on Permanent Hair Reduction

The purpose of this study is to learn more about how hair removal with lasers achieves, what appears to be, permanent hair reduction. Laser hair removal has become one of the most popular...

Microwave Ablation of Resectable Liver Tumors

The purpose of this protocol is to pathologically evaluate the destruction by microwave ablation of primary and metastatic liver tumors. The primary aim is to measure tissue destruction w...

Medical and Biotech [MESH] Definitions

Hair grooming and cleansing aids or other products meant for topical application to hair, usually human. They include sprays, bleaches, conditioners, rinses, shampoos, nutrient lotions, etc.

Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION; CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components.

The selective extraction of deciduous teeth during the stage of mixed dentition in accordance with the shedding and eruption of the teeth. It is done over an extended period to allow autonomous adjustment to relieve crowding of the dental arches during the eruption of the lateral incisors, canines, and premolars, eventually involving the extraction of the first premolar teeth. (Dorland, 28th ed)

Therapy assisted by the use of a horse and/or its movement, including equine-assisted psychotherapy, horseback riding, and hippotherapy.

Extraction of the fetus by means of obstetrical instruments.


Advertisement
 
Advertisement
 

Searches Linking to this Article