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The effectiveness of microwave-assisted extraction of proteins from human hair samples was evaluated. Extractions were performed from 2-mg hair samples in an extraction solution consisting of 25mM Tris-HCl (pH8.5), 2.6M thiourea, 5M urea, and 5% mercaptoethanol. During extraction, samples were exposed to microwave radiation (600W) for a specified incubation period (5-120min). The extraction efficiency of samples that had been incubated for 60min was similar to that of samples that had been heated at 50 degrees C for 24h using the conventional Shindai method.
Department of Chemistry, Chungnam National University, Daejeon, 305-764, South Korea.
This article was published in the following journal.
Name: Analytical biochemistry
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