Laser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers.
Summary of "Laser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers."
ABSTRACT:
BACKGROUND:
Bio-conjugated nanoparticles are important analytical tools with emerging biological and medical applications. In this context in situ conjugation of nanoparticles with biomolecules via laser ablation in aqueous media is a highly promising one step method for the production of functional nanoparticles resulting in high conjugation efficiencies. Increased yields are required particularly considering the conjugation of cost intensive biomolecules like RNA aptamers.
RESULTS:
Using a DNA aptamer directed against streptavidin in situ conjugation results in nanoparticles with diameters of approximately 9 nm exhibiting a high surface density of aptamers (98 aptamers per nanoparticle) and a maximal conjugation efficiency of 40.3%. We have demonstrated the functionality of the aptamer-conjugated nanoparticles in three independent analytical methods including an agglomeration-based colorimetric assay and solid-phase assays proving high aptamer activity. To demonstrate the general applicability of the in situ conjugation of gold nanoparticles with aptamers we have transferred the approach to a RNA aptamer directed against prostate specific membrane antigen (PSMA). Successful detection of PSMA in human prostate cancer tissue was achieved utilizing tissue microarrays.
CONCLUSIONS:
In comparison to conventional generation of bio-conjugated gold nanoparticles by chemical synthesis and subsequent bio-functionalization, the laser ablation-based in situ conjugation allows the rapid one-step production. Due to high conjugation efficiency and productivity in situ conjugation can be easily used for high throughput generation of gold nanoparticles conjugated with precious biomolecules like aptamers.
Affiliation
Journal Details
This article was published in the following journal.
Name: Journal of nanobiotechnology
ISSN: 1477-3155
Pages: 21
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20731831
- DOI: http://dx.doi.org/10.1186/1477-3155-8-21
Medical and Biotech [MESH] Definitions
Metal Nanoparticles
Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.
Keratectomy, Subepithelial, Laser-assisted
A surgical technique to correct REFRACTIVE ERRORS of the EYE, such as MYOPIA and ASTIGMATISM. In this method, a flap of CORNEAL EPITHELIUM is created by exposure of the area to dilute alcohol. The flap is lifted and then replaced after laser ablation of the subepithelial CORNEA.
Angioplasty, Laser
A technique utilizing a laser coupled to a catheter which is used in the dilatation of occluded blood vessels. This includes laser thermal angioplasty where the laser energy heats up a metal tip, and direct laser angioplasty where the laser energy directly ablates the occlusion. One form of the latter approach uses an EXCIMER LASER which creates microscopically precise cuts without thermal injury. When laser angioplasty is performed in combination with balloon angioplasty it is called laser-assisted balloon angioplasty (ANGIOPLASTY, BALLOON, LASER-ASSISTED).
Nanoparticles
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
Laser-doppler Flowmetry
A method of non-invasive, continuous measurement of MICROCIRCULATION. The technique is based on the values of the DOPPLER EFFECT of low-power laser light scattered randomly by static structures and moving tissue particulates.
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