DNA Barcoding of Panax Species.
Summary of "DNA Barcoding of Panax Species."
Ginsengs ( PANAX, Araliaceae) are among the plants best known for their medicinal properties. Many ginseng species are endangered due to over-exploitation of natural resources - a situation difficult to remedy while there are no reliable, practical methods for species identification. We screened eleven candidate DNA barcoding loci to establish an accurate and effective PANAX species identification system, both for commercial and conservation purposes. We used 95 ginseng samples, representing all the species in the genus. We found considerable differences in the performance of the potential barcoding regions. The sequencing of ATPF-ATPH was unsuccessful due to poly-N structures. The RBCL, RPOB, and RPOC1 regions were found to be mostly invariable, with only four to eight variable sites. Using MATK, PSBK-I, PSBM-TRND, RPS16 and NAD1, we could identify four to six out of eight considerably divergent species but only one to five out of nineteen clusters within the P. BIPINNATIFIDUS species group. PSBA-TRNH and ITS were the most variable loci, working very well both in species and cluster identifications. We demonstrated that the combination of PSBA-TRNH and ITS is sufficient for identifying all the species and clusters in the genus.
State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, the Chinese Academy of Sciences, Beijing, P. R. China.
This article was published in the following journal.
Name: Planta medica
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20803416
- DOI: http://dx.doi.org/10.1055/s-0030-1250166
Medical and Biotech [MESH] Definitions
A plant species of the genus PANAX. It contains damarane-type tetracyclic TRITERPENES. The common names of Sanchi or Tienchi are also used for Panax pseudoginseng which is distinguished in containing oleanane-type pentacyclic triterpenoids.
Dna Barcoding, Taxonomic
Techniques for standardizing and expediting taxonomic identification or classification of organisms that are based on deciphering the sequence of one or a few regions of DNA known as the "DNA barcode".
An araliaceous genus of plants that contains a number of pharmacologically active agents used as stimulants, sedatives, and tonics, especially in traditional medicine. Sometimes confused with Siberian ginseng (ELEUTHEROCOCCUS).
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Gb Virus A
A species of virus (unassigned to a genus) in the family FLAVIVIRIDAE, that have been identified in at least six species of New World monkeys. They do not cause HEPATITIS in the host or other susceptible species.
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