DNA Barcoding of Panax Species.
Summary of "DNA Barcoding of Panax Species."
Ginsengs ( PANAX, Araliaceae) are among the plants best known for their medicinal properties. Many ginseng species are endangered due to over-exploitation of natural resources - a situation difficult to remedy while there are no reliable, practical methods for species identification. We screened eleven candidate DNA barcoding loci to establish an accurate and effective PANAX species identification system, both for commercial and conservation purposes. We used 95 ginseng samples, representing all the species in the genus. We found considerable differences in the performance of the potential barcoding regions. The sequencing of ATPF-ATPH was unsuccessful due to poly-N structures. The RBCL, RPOB, and RPOC1 regions were found to be mostly invariable, with only four to eight variable sites. Using MATK, PSBK-I, PSBM-TRND, RPS16 and NAD1, we could identify four to six out of eight considerably divergent species but only one to five out of nineteen clusters within the P. BIPINNATIFIDUS species group. PSBA-TRNH and ITS were the most variable loci, working very well both in species and cluster identifications. We demonstrated that the combination of PSBA-TRNH and ITS is sufficient for identifying all the species and clusters in the genus.
Affiliation
State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, the Chinese Academy of Sciences, Beijing, P. R. China.
Journal Details
This article was published in the following journal.
Name: Planta medica
ISSN: 1439-0221
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20803416
- DOI: http://dx.doi.org/10.1055/s-0030-1250166
Medical and Biotech [MESH] Definitions
Panax Notoginseng
A plant species of the genus PANAX. It contains damarane-type tetracyclic TRITERPENES. The common names of Sanchi or Tienchi are also used for Panax pseudoginseng which is distinguished in containing oleanane-type pentacyclic triterpenoids.
Dna Barcoding, Taxonomic
Techniques for standardizing and expediting taxonomic identification or classification of organisms that are based on deciphering the sequence of one or a few regions of DNA known as the "DNA barcode".
Panax
An araliaceous genus of plants that contains a number of pharmacologically active agents used as stimulants, sedatives, and tonics, especially in traditional medicine. Sometimes confused with Siberian ginseng (ELEUTHEROCOCCUS).
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Gb Virus A
A species of virus (unassigned to a genus) in the family FLAVIVIRIDAE, that have been identified in at least six species of New World monkeys. They do not cause HEPATITIS in the host or other susceptible species.
PubMed Articles
DNA barcoding using the mitochondrial cytochromecoxidase subunit I (cox-1) gene has recently gained popularity as a tool for species identification of a variety of taxa. The primary objective of our r...
DNA barcoding of Caenogastropoda along coast of China based on the COI gene.
DNA barcoding provides an efficient method for species-level identifications. In this study, we have amplified partial sequences of mitochondrial cytochrome c oxidase I (COI) gene from 110 specimens o...
DNA barcoding, phylogenetic relationships and speciation of snappers (genus Lutjanus).
The phylogenetic relationships of 13 snapper species from the South China Sea have been established using the combined DNA sequences of three full-length mitochondrial genes (COI, COII and CYTB) and t...
The Pánuco-Tamesí complex in eastern Mexico is globally recognized as an important ecoregion due to its high level of endemism. In this study, DNA barcodes were generated for 152 individuals of 31 s...
Oysters (family Ostreidae), with high levels of phenotypic plasticity and wide geographic distribution, are a challenging group for taxonomists and phylogenetics. As a useful tool for molecular specie...
Clinical Trials
Cognitive, Emotional, Physical and Psychosocial Effects of Panax Quinquefolius L
The purpose of this study is to determine the efficacy and safety of REMEMBER-fX (HT1001, an extract of Panax quinquefolius) in a human sample using standard clinical neuropsychological in...
The study is a 2 phase double blind randomized placebo control trial. The objective is to asses the metabolic and therapeutic effects of American Ginseng (Panax quinquefolius L.) extract a...
Effect of Panax Ginseng on the Cognitive Performance in Alzheimer’s Disease
We investigate the clinical efficacy of Panax ginseng in Alzheimer’s disease (AD).
The aim of the study is to investigate the effect of inhaled apocynin on ROS (reactive oxygen species) and NOS (reactive nitrogen species) synthesis in 10 nonsmoking mild asthmatics. Effec...
Evaluation and Standardization of Ginseng and it's Components for Blood Pressure Regulation
Ginseng has been used for many years in a wide array of anecdotal medicinal properties. Animal and limited clinical research points to the vascular effects of Korean Red Ginseng (KRG). The...
