Bioluminescence imaging correlates with tumor progression in an orthotopic mouse model of lung cancer.
Summary of "Bioluminescence imaging correlates with tumor progression in an orthotopic mouse model of lung cancer."
To determine whether bioluminescence imaging of human lung cancer cells growing in an orthotopic murine model provides a sensitive tool for monitoring tumor progression in athymic nude mice.
Human lung cancer (A549) cells were stably transfected with the firefly luciferase gene and inoculated into the right lung of athymic nude mice. Seven days after inoculation tumor growth was evaluated using the Kodak in-vivo Imaging System FX and continued to be monitored on a weekly basis.
In duplicate experiments, human lung cancer tumors formed in 90% of animal's injected orthotopically. The mean intensity of the bioluminescence signal emitted from the lung cancer cells increased logarithmically during the course of study. Mice with positive bioluminescence signaling had confirmed tumors by microscopic histological analysis. Bioluminescence activity had a strong correlation with the tumor volume as determined histologically.
Bioluminescence intensity directly correlates with tumor volume and therefore offers a reliable approach for detecting and monitoring the growth of human lung cancer cells in orthotopic murine models.
M. D. Anderson Cancer Center Orlando, Cancer Research Institute, 6900 Lake Nona Boulevard, 5th Floor, Orlando, FL 32827, USA.
This article was published in the following journal.
Name: Surgical oncology
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20801643
- DOI: http://dx.doi.org/10.1016/j.suronc.2010.07.008
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Medical and Biotech [MESH] Definitions
The use of molecularly targeted imaging probes to localize and/or monitor biochemical and cellular processes via various imaging modalities that include RADIONUCLIDE IMAGING; ULTRASONOGRAPHY; MAGNETIC RESONANCE IMAGING; fluorescence imaging; and MICROSCOPY.
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Genetic loci responsible for the encoding of minor lymphocyte stimulatory antigens. There are at least two unlinked loci (in the mouse) and they appear to be separate from the MAJOR HISTOCOMPATIBILITY COMPLEX and MINOR HISTOCOMPATIBILITY LOCI. The mouse mammary tumor virus (see MAMMARY TUMOR VIRUS, MOUSE) has the ability to integrate into these loci. The antigens induce strong T-cell proliferative responses in mixed lymphocyte reactions.
A transplantable, poorly differentiated malignant tumor which appeared originally as a spontaneous breast carcinoma in a mouse. It grows in both solid and ascitic forms.
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