Slow-release hrBMP-2 embedded within electrospun scaffolds for regeneration of bone defect: in vitro and in vivo evaluation.
Summary of "Slow-release hrBMP-2 embedded within electrospun scaffolds for regeneration of bone defect: in vitro and in vivo evaluation."
The design of mat-like scaffolds slow releasing BMP-2, retaining bone regeneration functions has been a major challenge in tissue engineering. This study aimed to develop core-shell fiber scaffolds releasing BMP-2 to support bone regeneration. BMP-2 was incorporated in an aqueous core solution of poly(ethylene oxide) (PEO) while the shell solution was made of polycaprolactone (PCL) blended with poly(ethylene glycol) (PEG). This blending induced pores in the shell which pronouncedly affected the movement of proteins out of the fibers. BMP-2 release profiles were monitored. In vitro bioactivity of BMP-2 released from the scaffolds was assessed using human MSCs by measuring alkaline phosphatase activity. Bone regeneration capabilities were demonstrated by implanting the BMP-2-embedded scaffolds in rat cranial defect model followed by microCT analysis. The degree of the fiber's shell porosity, highly correlative with the slow and fast release patterns of BMP-2 were found to be dependent on the relative amount of PEG within the shell. In vitro assays of scaffolds manifesting the slow release pattern have revealed significant (~9 fold) increase in alkaline phosphatase activity, compared to fast BMP-2 releasing scaffolds. Likewise, in vivo studies have revealed significant bone regeneration in cranial defects of scaffold implants with rhBMP-2 with slow release pattern.
Affiliation
Technion - Israel Institute of Technology, Faculty of Medicine - Department of Anatomy and Cell Biology, Haifa, Israel; mdsamer@tx.technion.ac.il.
Journal Details
This article was published in the following journal.
Name: Tissue engineering. Part A
ISSN: 1937-335X
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20799887
- DOI: http://dx.doi.org/10.1089/ten.TEA.2010.0250
Medical and Biotech [MESH] Definitions
Tissue Scaffolds
Cell growth support structures composed of BIOCOMPATIBLE MATERIALS. They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses.
Chemoembolization, Therapeutic
Administration of antineoplastic agents together with an embolizing vehicle. This allows slow release of the agent as well as obstruction of the blood supply to the neoplasm.
Guided Tissue Regeneration
Procedures for enhancing and directing tissue repair and renewal processes, such as BONE REGENERATION; NERVE REGENERATION; etc. They involve surgically implanting growth conducive tracks or conduits (TISSUE SCAFFOLDING) at the damaged site to stimulate and control the location of cell repopulation. The tracks or conduits are made from synthetic and/or natural materials and may include support cells and induction factors for CELL GROWTH PROCESSES; or CELL MIGRATION.
Platelet-rich Plasma
A preparation consisting of PLATELETS concentrated in a limited volume of PLASMA. This is used in various surgical tissue regeneration procedures where the GROWTH FACTORS in the platelets enhance wound healing and regeneration.
Plasma Skin Regeneration
A cosmetic technique that uses PLASMA GASES in therapeutic treatment to help achieve skin REJUVENATION or REGENERATION and delay SKIN AGING.
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