Translocation of Pseudomonas aeruginosa from the intestinal tract is mediated by the binding of ExoS to an Na, K-ATPase regulator, FXYD3.
Summary of "Translocation of Pseudomonas aeruginosa from the intestinal tract is mediated by the binding of ExoS to an Na, K-ATPase regulator, FXYD3."
The intestinal tract is considered the most important reservoir of Pseudomonas aeruginosa in intensive care units. Gut colonization by P. aeruginosa underlies the development of invasive infections such as gut-derived sepsis. Intestinal colonization by P. aeruginosa is associated with higher ICU mortality rates. Translocation of endogenous P. aeruginosa from the colonized intestinal tract is an important pathogenic phenomenon. We identify here bacterial and host proteins associated with bacterial penetration through the intestinal epithelial barrier. We first show by comparative genomic hybridization analysis that the exoS gene encoding the type III effector protein, ExoS, is specifically detected in a clinical isolate that showed higher virulence in silkworms following midgut injection. We further show using a silkworm oral infection model that exoS is required both for virulence andfor bacterial translocation from the midgut to the hemolymph. Using a bacterial two-hybrid screen, we show that the mammalian factor, FXYD3, which co-localizes with and regulates the function of Na, K-ATPase, directly binds ExoS. A pull-down assay revealed that ExoS binds to the transmembrane domain of FXYD3, which also interacts with Na, K-ATPase. Na, K-ATPase controls the structure and barrier function of tight junctions in epithelial cells. Collectively, our results suggest that ExoS facilitates P. aeruginosa penetration through the intestinal epithelial barrier by binding to FXYD3 and thereby impairing the defense function of tight junctions against bacterial penetration.
Department of Microbiology and Infection Control Science, and Educational and Research Center for Clinical Pharmacy, Kyoto Pharmaceutical University, Yamashina, Kyoto 607-8414, Japan.
This article was published in the following journal.
Name: Infection and immunity
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20805335
- DOI: http://dx.doi.org/10.1128/IAI.00428-10
Medical and Biotech [MESH] Definitions
A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.
The passage of viable bacteria from the gastrointestinal tract to extra-intestinal sites, such as the mesenteric lymph node complex, liver, spleen, kidney, and blood. Factors that promote bacterial translocation include overgrowth with gram-negative enteric bacilli, impaired host immune defenses, and injury to the intestinal mucosa resulting in increased intestinal permeability. These mechanisms can act in concert to promote synergistically the systemic spread of indigenous translocating bacteria to cause lethal sepsis.
Antibiotic pigment produced by Pseudomonas aeruginosa.
Gram-negative bacteria isolated from infections of the respiratory and intestinal tracts and from the buccal cavity, intestinal tract, and urogenital tract. They are probably part of the normal flora of man and animals.
Bacteriocins elaborated by mutant strains of Pseudomonas aeruginosa. They are protein or protein-lipopolysaccharide complexes lethal to other strains of the same or related species.
Pseudomonas aeruginosa is a common bacterium that can cause disease. The versatility of Pseudomonas aeruginosa enables the organism to infect damaged tissues or those with reduced immunity which cause...
Epithelial to mesenchymal transition has been implicated in the dysregulated epithelial wound repair that contributes to obliterative bronchiolitis after lung transplant. Acquisition of Pseudomonas ae...
Chromium removal and its association with rhamnolipid production in Pseudomonas spp. were investigated. Three Pseudomonas spp. isolates (P. aeruginosa 78, P. aeruginosa 99, and P. stutzeri T3) were in...
ABSTRACT: BACKGROUND: Urinary catheters are associated, commonly with bacteriuria and frequently with urinary tract infection. Tamm-Horsfall Protein (THP) is urine's most abundant protein and is known...
A novel Pseudomonas aeruginosa lytic bacteriophage (phage), PA1Ø, was isolated, and its genome was sequenced completely. This phage is able to lyse not only P. aeruginosa but also Staphylococcus aure...
Preventing carriage of potentially pathogenic micro-organisms from the aerodigestive tract is an infection control strategy used to reduce the occurrence of nosocomial infections - includi...
This is an open-label, single arm (uncontrolled) study in subjects suffering from cystic fibrosis, who have completed their study participation in CTBM100C2303 (all visits) and who were pr...
The objectives of this open study are to assess the safety, tolerability, pharmacokinetics and clinical outcome of patients who have HAP caused by Pseudomonas aeruginosa serotype O11 after...
OBJECTIVES: I. Assess the efficacy of monthly intravenous mucoid exopolysaccharide Pseudomonas aeruginosa immune globulin (MEP IGIV) given over 1 year in reducing the frequency of acute p...
The purpose of this study is to determine whether the nasal inhalation of Colistin is effective to decrease the Pseudomonas aeruginosa bacterial count in the nasal lavage fluid.