Development and validation of fixed-time method for the determination of isoxsuprine hydrochloride in commercial dosages forms.
Summary of "Development and validation of fixed-time method for the determination of isoxsuprine hydrochloride in commercial dosages forms."
The main aim of this work was to develop a kinetic spectrophotometric method for the quantitative analysis of isoxsuprine hydrochloride in commercial tablets. The method is based on the reaction of isoxsuprine hydrochloride (ISx) with hydroxylamine hydrochloride and ammonium cerium (IV) nitrate in sulphuric acid medium at room temperature which resulted in the formation of yellow-coloured product peaking at 380 nm. The reaction is followed spectrophotometrically by measuring the absorbance as a function of time. Fixed time method (DeltaA = A(4)-A(2), where A(2) and A(4) refer to absorbance measurements taken at 2 and 4 min, respectively) was adopted for constructing the calibration curve which was found to be linear over the concentration range of 30-80 microgmL(-1) with molar absorptivity of 5.95 x 10(3) L mol(-1) cm(-1). The method has been applied successfully to the determination of isoxsuprine hydrochloride in tablets. Statistical comparison (point and interval hypothesis tests) of the results showed that there is no significant difference between the proposed method and reference method. Copyright (c) 2010 John Wiley & Sons, Ltd.
Affiliation
Department of Chemistry, Aligarh Muslim University, Aligarh-202002 (U.P.) India.
Journal Details
This article was published in the following journal.
Name: Drug testing and analysis
ISSN: 1942-7611
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20812296
- DOI: http://dx.doi.org/10.1002/dta.153
Medical and Biotech [MESH] Definitions
Sex Determination Analysis
Validation of the SEX of an individual by inspection of the GONADS and/or by genetic tests.
Sex Determination (analysis)
Validation of the SEX of an individual by inspection of the GONADS and/or by genetic tests.
Sex Determination By Skeleton
Validation of the sex of an individual by means of the bones of the SKELETON. It is most commonly based on the appearance of the PELVIS; SKULL; STERNUM; and/or long bones.
Sex Determination Processes
The mechanisms by which the SEX of an individual's GONADS are fixed.
Blood Circulation Time
Determination of the shortest time interval between the injection of a substance in the vein and its arrival at some distant site in sufficient concentration to produce a recognizable end result. It represents approximately the inverse of the average velocity of blood flow between two points.
PubMed Articles
In this study, we investigated a simple, sensitive and reliable liquid chromatography-fluorescence detection method for the determination of memantine hydrochloride in rat plasma which was based on de...
Determination of dobutamine hydrochloride by enzymatic catalytic spectrofluorimetry.
A highly sensitive and simple spectrofluorimetric method for the determination of dobutamine hydrochloride based on its inhibitory effect on the hemoglobin-catalyzed reaction of H2 O2 and l-tyrosine w...
Development and validation of a paediatric oral formulation of clonidine hydrochloride.
Many drugs are unavailable in suitable paediatric dosage forms. We describe the development and validation of a stable paediatric oral formulation of clonidine hydrochloride 50 μg/ml, allowing indivi...
A simple and sensitive extractive spectrophotometric method have been developed and validated for determination of amantadine hydrochloride (AM), memantine hydrochloride (MM) and rimantadine hydrochlo...
Besifloxacin hydrochloride is a novel chiral broad-spectrum fluoroquinolone developed for the treatment of bacterial conjunctivitis. R-besifloxacin hydrochloride is used in clinics as a consequence of...
Clinical Trials
Evaluation of a Bioimpedance Method for Determination of Dry Weight in Dialysis Patients
The purpose of this study is the evaluation of a bioimpedance method for determination of dry weight in dialysis patients. Additionally normal tissue hydration in non-Dialysis patients is...
Study on Costs and Effects of Waiting Time in Total Hip and Knee Replacements
The aim of this study is to compare the cost-effectiveness of major joint replacement in patients with short and non-fixed (control group) waiting time (WT). After being placed on the wait...
Non-Invasive Determination of Cardiac Output by Inertgas Rebreathing Method
The cardiac output (CO) is an important parameter in the diagnosis and therapy of cardiac diseases. The current standard methods for the determination of the CO, however, are either invasi...
Comparing Effects of Two Fixed Combinations Ophthalmic Solutions on Ocular Blood Flow
A 10 week evaluation, crossover design study including a 3 week washout period between treatments, to determine the effects of fixed combination brimonidine tartrate 0.2%/timolol maleate 0...
Accurate length determination of root canal is essential in root canal therapy. Radiography has been the conventional method for this measurement but recently several in-vitro studies have...
