Thermophilic laccase from xerophyte species Opuntia vulgaris.
Summary of "Thermophilic laccase from xerophyte species Opuntia vulgaris."
Two laccase temperature isoforms capable of oxidizing phenolic compounds to quinones were isolated and purified to homogeneity from the cladodes of the xerophyte species Opuntia vulgaris. These catalytically active proteins exhibit apparent molecular masses of 137 and 90 kDa. Under reducing conditions, both isoforms yielded a subunit molecular mass of 43 kDa, suggesting that the enzyme is a multimer of the 43 kDa subunit. The 137 kDa isoform when heated at 80 degrees C for 3min generated three polypeptide bands on activity stained polyacrylamide gels exhibiting 137, 90 and 43 kDa molecular forms. All isoforms of the enzyme exhibited an optimum pH of 10 when 2,6-dimethoxyphenol was used as a substrate. The optimum temperature of the 137 kDa enzyme form was noted to be 80 degrees C and that of the 90 kDa enzyme form was 70 degrees C. Denaturation kinetics of both the laccase isoforms carried out at their respective optimum temperatures for 30 min exhibited enzyme activity in excess of their t(1/2) values throughout the assay period. The K(m) for the 137 kDa form was determined to be 2.2 +/- 0.3 mm and the V(max) was 2.8 +/- 0.2 IU/mL. These high temperature stable laccase isoforms having alkaline pH optima can find significant industrial use. Copyright (c) 2010 John Wiley & Sons, Ltd.
Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Puducherry 605014, India.
This article was published in the following journal.
Name: Biomedical chromatography : BMC
The genus Opuntia (Cactaceae) includes different plants well adapted to arid and semi-arid zones. These species are cultivated under restricted growth conditions, not suitable for the growth of other ...
Thermophilic fungi are of wide interest due to their potential to produce heat-tolerant enzymes for biotechnological processes. However, the taxonomy of such organisms remains obscure, especially give...
Bean plants from the Phaseolus genus are widely consumed and represent a nitrogen source for human nutrition. They provide biological fertilization by establishing root nodule symbiosis with nitrogen-...
To find markers that distinguish the different Cactaceae species, by using near infrared Raman spectroscopy and scanning electron microscopy, we studied the occurrence, in the stem, of solid deposits ...
Poly(vinyl alcohol) (PVA) microspheres were prepared by inverse suspension crosslinked method, with glutaraldehyde as a crosslinking agent. PVA microspheres activated with aldehyde groups were employe...
The purpose of this study is to assess the effectiveness of IDP-110 in treating patients with acne vulgaris.
The purpose of this study is to determine whether KC706 is effective in the prevention and healing of blisters in patients with pemphigus vulgaris, while the patient remains on stable dose...
The purpose of this study is to assess the safety and effectiveness of IDP-107 in treating patients with acne vulgaris.
The purpose of the study is to find out about the effectiveness and the safety of an investigational drug called etanercept (Enbrel) to treat pemphigus vulgaris. Pemphigus vulgaris is a po...
Patients with vitiligo vulgaris are treated with Pimecrolimus twice a day during 6 months. After a baseline visit, patient returns for a control visit after 3 and 6 months of treatment. On...
Medical and Biotech [MESH] Definitions
A plant genus of the family CACTACEAE. Species with cylindrical joints are called Cholla; flat jointed ones are Prickly-pear.
A species of gram-negative bacteria and nitrogen innoculant of PHASEOLUS VULGARIS.
A species of green microalgae in the family Chlorellaceae. It is used as a model organism for PHOTOSYNTHESIS, and as a food supplement (DIETARY SUPPLEMENTS).
A species of thermophilic CAMPYLOBACTER found in healthy seagulls and causing ENTERITIS in humans.
A species of the Beta genus. Cultivars are used as a source of beets (root) or chard (leaves).