Advertisement

Topics

Thermophilic laccase from xerophyte species Opuntia vulgaris.

06:00 EDT 3rd September 2010 | BioPortfolio

Summary of "Thermophilic laccase from xerophyte species Opuntia vulgaris."

Two laccase temperature isoforms capable of oxidizing phenolic compounds to quinones were isolated and purified to homogeneity from the cladodes of the xerophyte species Opuntia vulgaris. These catalytically active proteins exhibit apparent molecular masses of 137 and 90 kDa. Under reducing conditions, both isoforms yielded a subunit molecular mass of 43 kDa, suggesting that the enzyme is a multimer of the 43 kDa subunit. The 137 kDa isoform when heated at 80 degrees C for 3min generated three polypeptide bands on activity stained polyacrylamide gels exhibiting 137, 90 and 43 kDa molecular forms. All isoforms of the enzyme exhibited an optimum pH of 10 when 2,6-dimethoxyphenol was used as a substrate. The optimum temperature of the 137 kDa enzyme form was noted to be 80 degrees C and that of the 90 kDa enzyme form was 70 degrees C. Denaturation kinetics of both the laccase isoforms carried out at their respective optimum temperatures for 30 min exhibited enzyme activity in excess of their t(1/2) values throughout the assay period. The K(m) for the 137 kDa form was determined to be 2.2 +/- 0.3 mm and the V(max) was 2.8 +/- 0.2 IU/mL. These high temperature stable laccase isoforms having alkaline pH optima can find significant industrial use. Copyright (c) 2010 John Wiley & Sons, Ltd.

Affiliation

Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Puducherry 605014, India.

Journal Details

This article was published in the following journal.

Name: Biomedical chromatography : BMC
ISSN: 1099-0801
Pages:

Links

DeepDyve research library

PubMed Articles [8196 Associated PubMed Articles listed on BioPortfolio]

Shared Physiological Traits of Exophiala Species in Cold-Blooded Vertebrates, as Opportunistic Black Yeasts.

Several species of the genus Exophiala are found as opportunistic pathogens on humans, while others cause infections in cold-blooded waterborne vertebrates. Opportunism of these fungi thus is likely t...

Laccase multigene families in Agaricomycetes.

Here we present the results of the exploration of laccase multigene families (MGFs) in basidiomycetous fungi from different taxonomic groups using a next generation sequencing (NGS) technology. In our...

Purification and Characterization of a White Laccase with Pronounced Dye Decolorizing Ability and HIV-1 Reverse Transcriptase Inhibitory Activity from Lepista nuda.

A strain LN07 with high laccase yield was identified as basidiomycete fungus Lepista nuda from which a white laccase without type I copper was purified and characterized. The laccase was a monomeric p...

On Dioxygen Permeation of MaL Laccase from the Thermophilic Fungus Melanocarpus albomyces. An all-Atom Molecular Dynamics Investigation.

Described here are biased MD simulations of O2 egress from the active center of MaL laccase toward the bulk solvent. Parameterization of the set of four Cu(II) ions, in the framework of CHARMM-36 FF, ...

Phylogeographic past and invasive presence of Arion pest slugs in Europe.

Arion vulgaris (syn. A. lusitanicus) is the most destructive pest slug in Europe. The species has been regarded a classic case of an ongoing biological invasion with negative economic and ecological i...

Clinical Trials [414 Associated Clinical Trials listed on BioPortfolio]

A Clinical Study Evaluating the Safety and Efficacy of IDP-110 in Patients With Acne Vulgaris

The purpose of this study is to assess the effectiveness of IDP-110 in treating patients with acne vulgaris.

Efficacy Study in Patients With Acne Vulgaris.

Safety and efficacy study in patients with acne vulgaris

Use of KC706 for the Treatment of Pemphigus Vulgaris

The purpose of this study is to determine whether KC706 is effective in the prevention and healing of blisters in patients with pemphigus vulgaris, while the patient remains on stable dose...

A Clinical Study Evaluating the Safety and Efficacy of IDP-107 in Patients With Acne Vulgaris

The purpose of this study is to assess the safety and effectiveness of IDP-107 in treating patients with acne vulgaris.

Study of Etanercept (Enbrel) in the Treatment of Pemphigus Vulgaris

The purpose of the study is to find out about the effectiveness and the safety of an investigational drug called etanercept (Enbrel) to treat pemphigus vulgaris. Pemphigus vulgaris is a po...

Medical and Biotech [MESH] Definitions

A plant genus of the family CACTACEAE. Species with cylindrical joints are called Cholla; flat jointed ones are Prickly-pear.

A species of gram-negative bacteria and nitrogen innoculant of PHASEOLUS VULGARIS.

A species of green microalgae in the family Chlorellaceae. It is used as a model organism for PHOTOSYNTHESIS, and as a food supplement (DIETARY SUPPLEMENTS).

A species of thermophilic CAMPYLOBACTER found in healthy seagulls and causing ENTERITIS in humans.

A species of the Beta genus. Cultivars are used as a source of beets (root) or chard (leaves).

Quick Search
Advertisement
 


DeepDyve research library

Relevant Topics

Enzymes
Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...

Assays
An assay is an analytic procedure for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity.  This can be a drug or biochemical substance or a cell in an organism or organic sample. ...


Searches Linking to this Article