3D poly(1,8 Octanediol-co-Citrate) (POC) scaffold pore shape and permeability effects on sub-cutaneous in vivo chondrogenesis using primary chondrocytes.
Summary of "3D poly(1,8 Octanediol-co-Citrate) (POC) scaffold pore shape and permeability effects on sub-cutaneous in vivo chondrogenesis using primary chondrocytes."
The objective of this study was to evaluate the coupled effects of 3D POC scaffold pore shape and permeability on chondrogenesis using primary chondrocytes in vivo. Chondrogenesis was characterized by cartilage matrix formation via sulfated glycosaminoglycan (sGAG) quantification, relative mRNA expressions of cartilage related proteins type 1, 2, 10 collagens, aggrecan, and matrix metalloproteinase 13 & 3 (MMP13, MMP3), and tissue/scaffold construct compressive mechanical properties. The low permeable design with spherical pore shapes showed a significantly higher increase in cartilage matrix formation over 6 weeks in vivo than the high permeable design with a cubical pore shape. This increase in cartilage matrix synthesis corresponded with increases in mechanical compressive nonlinear elastic properties and histological data demonstrating darker red Safranin-O staining. There were higher mRNA expressions for both cartilage specific proteins and matrix degradation proteins in the high permeable design, resulting in overall less sGAG retained in the high permeable scaffold compared with the low permeable scaffold. Controlled POC scaffold spherical pore shape and low permeability correlated with significantly increased cartilage matrix production using primary seeded chondrocytes. These results indicate that the low permeable design with spherical pore shape provided a better microenvironment for chondrogenesis than the high permeable design with cubical pore shape. Thus, scaffold architecture design and material may have significant impact on the success of matrix based clinical cartilage repair strategies.
Affiliation
Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, 48109-2125, USA.
Journal Details
This article was published in the following journal.
Name: Acta biomaterialia
ISSN: 1878-7568
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20807597
- DOI: http://dx.doi.org/10.1016/j.actbio.2010.08.027
Medical and Biotech [MESH] Definitions
Poly(a)-binding Protein Ii
A poly(A) binding protein that is involved in promoting the extension of the poly A tails of MRNA. The protein requires a minimum of ten ADENOSINE nucleotides in order for binding to mRNA. Once bound it works in conjunction with CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR to stimulate the rate of poly A synthesis by POLY A POLYMERASE. Once poly-A tails reach around 250 nucleotides in length poly(A) binding protein II no longer stimulates POLYADENYLATION. Mutations within a GCG repeat region in the gene for poly(A) binding protein II have been shown to cause the disease MUSCULAR DYSTROPHY, OCULOPHARYNGEAL.
Atp Citrate (pro-s)-lyase
An enzyme that, in the presence of ATP and COENZYME A, catalyzes the cleavage of citrate to yield acetyl CoA, oxaloacetate, ADP, and ORTHOPHOSPHATE. This reaction represents an important step in fatty acid biosynthesis. This enzyme was formerly listed as EC 4.1.3.8.
Nucleocytoplasmic Transport Proteins
Proteins involved in the process of transporting molecules in and out the cell nucleus. Included here are: NUCLEOPORINS, which are membrane proteins that form the NUCLEAR PORE COMPLEX; KARYOPHERINS, which carry molecules through the nuclear pore complex; and proteins that play a direct role in the transport of karyopherin complexes through the nuclear pore complex.
Poly(a)-binding Protein I
A poly(A) binding protein that has a variety of functions such as mRNA stabilization and protection of RNA from nuclease activity. Although poly(A) binding protein I is considered a major cytoplasmic RNA-binding protein it is also found in the CELL NUCLEUS and may be involved in transport of mRNP particles.
Citrate (si)-synthase
Enzyme that catalyzes the first step of the tricarboxylic acid cycle (CITRIC ACID CYCLE). It catalyzes the reaction of oxaloacetate and acetyl CoA to form citrate and coenzyme A. This enzyme was formerly listed as EC 4.1.3.7.
PubMed Articles
Poly(1,8-octanediol-co-citrate) (POC) is a biocompatible, biodegradable elastomer with potential application for soft tissue applications such as cartilage. For chondrogenesis, permeability is a scaff...
Poly(octanediol-co-citrate) elastomers containing high loading of lidocaine were synthesized at temperatures below 100 °C by means of using deep eutectic mixtures of 1,8-octanediol and lidocaine. The...
A Survey of Methods for the Evaluation of Tissue Engineering Scaffold Permeability.
The performance of porous scaffolds for tissue engineering (TE) applications is evaluated, in general, in terms of porosity, pore size and distribution, and pore tortuosity. These descriptors are ofte...
Generally, solid-freeform fabricated scaffolds show controllable pore structure (pore size, porosity, pore-connectivity, and permeability) and mechanical properties by using computer-aided techniques....
Permeability analysis of scaffolds for bone tissue engineering.
Porous artificial bone substitutes, especially bone scaffolds coupled with osteobiologics, have been developed as an alternative to the traditional bone grafts. The bone scaffold should have a set of...
Clinical Trials
Phase I Oncovir Poly IC:LC and NY-ESO-1/gp100/MART
The purpose of this study is to determine what side effects CP 870,893 may cause when given with an immune stimulant called Oncovir poly IC:LC along with a melanoma vaccine. The CP 870,893...
Removal of Ligand-bound Iron During Intravenous (IV) Iron Administration in Hemodialysis (HD)
The study's hypothesis is that HD with large pore dialyzers remove to the dialysate iron which is bound to potentially dialyzable ligands, after it's release from intravenous iron compound...
Effects of Ethanol on Intestinal Permeability and Integrity
Alcohol consumption is a major health problem worldwide. It affects all systems of the body especially the gastrointestinal tract. Acute or chronic alcohol consumption has deleterious effe...
Alveolar Ridge Preservation With Polycaprolactone Scaffold for Implant Site Development
It has been shown through research that alveolar ridges (Part of jaw bone holding teeth) tend to lose width and height after extraction of teeth. Lost in height and width of the ridge can...
This is a pilot vaccine study in adults with either WHO grade II astrocytoma or oligoastrocytoma. The purpose of this study is test the safety and efficacy of an experimental tumor vaccine...
