Protein oxidation enhances hydration but suppresses water-holding capacity in porcine longissimus muscle.
Summary of "Protein oxidation enhances hydration but suppresses water-holding capacity in porcine longissimus muscle."
Pork longissimus muscle was oxidized at 4 °C by mixed 10 μM FeCl(3)/100 μM ascorbate with 1, 5, 10, 20, 30, 40, or 50 mM H(2)O(2) (pH 6.2). Oxidation with >1 mM H(2)O(2) for 40 min significantly (P < 0.05) enhanced hydration of muscle samples, whereas oxidation with 40 and 50 mM H(2)O(2) for 2 min or with 20 mM H(2)O(2) for 40 min caused pronounced declines in water-holding capacity and product yield. The changes coincided with marked increases in the protein carbonyl content, TBARS formation, and cross-linking of both myofibrillar and sarcoplasmic proteins. Dye-tracing tests showed that the enhanced hydration at >1 mM H(2)O(2) was due to facilitated water diffusion into muscle tissue. This result was strongly corroborated by microscopic images that illustrated enlargements of intercellular spacing, that is, gaps, in oxidized muscle tissue, which served as canals for water diffusion.
Affiliation
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Journal Details
This article was published in the following journal.
Name: Journal of agricultural and food chemistry
ISSN: 1520-5118
Pages: 10697-704
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20806938
- DOI: http://dx.doi.org/10.1021/jf102043k
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