A laboratory evaluation of the antibacterial and cytotoxic effect of Liquorice when used as root canal medicament.
Summary of "A laboratory evaluation of the antibacterial and cytotoxic effect of Liquorice when used as root canal medicament."
Badr AE, Omar N, Badria FA A laboratory evaluation of the antibacterial and cytotoxic effect of Liquorice when used as root canal medicament. International Endodontic Journal00. Abstract Aim To evaluate the antibacterial and cytotoxic effects of Liquorice as a root canal medicament and to compare its action to the commonly used root canal medicament calcium hydroxide Ca(OH)(2). Methodology The antibacterial effect of Liquorice and Ca(OH)(2) either separately or in combination was investigated against Enterococcus faecalis. Agar-well diffusion methods, broth microdilution tests and biofilm susceptibility assays were used to determine the antibacterial activity. Human periodontal ligament fibroblast tissue culture was used to assess the cytotoxicity of the preparations under investigation. Results Liquorice extract either by itself or in combination with Ca(OH)(2) had a significant inhibitory effect against Enterococcus faecalis compared with that of Ca(OH)(2) alone. The use of Liquorice extract followed by Liquorice/Ca(OH)(2) mixture retained significantly more viable periodontal ligament cells than Ca(OH)(2), which had a strong lethal effect on the cells. Conclusion Liquorice extract either separately or as Liquorice/Ca(OH)(2) mixture had a potent bactericidal effect against Enterococcus faecalis and retained compatibility with fibroblasts in tissue culture compared to the commonly used root canal medicament Ca(OH)(2).
Affiliation
Department of Conservative Dentistry and Endodontics, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.
Journal Details
This article was published in the following journal.
Name: International endodontic journal
ISSN: 1365-2591
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20812941
- DOI: http://dx.doi.org/10.1111/j.1365-2591.2010.01794.x
Medical and Biotech [MESH] Definitions
Endpoint Determination
Establishment of the level of a quantifiable effect indicative of a biologic process. The evaluation is frequently to detect the degree of toxic or therapeutic effect.
No-observed-adverse-effect Level
The highest dosage administered that does not produce toxic effects. The NOAEL will depend on how closely dosages are spaced (lowest-observed-adverse-effect level and no-observed-effect level) and the number of animals examined. The ultimate objective is usually to determine not the "safe" dosage in laboratory animals but the "safe" dosage for humans. Therefore, the extrapolation most often required of toxicologists is from high-dosage studies in laboratory animals to low doses in humans. (Casarett and Doull's Toxicology: The Basic Science of Poisons, 4th ed)
Placebo Effect
An effect usually, but not necessarily, beneficial that is attributable to an expectation that the regimen will have an effect, i.e., the effect is due to the power of suggestion.
Cytotoxicity Tests, Immunologic
The demonstration of the cytotoxic effect on a target cell of a lymphocyte, a mediator released by a sensitized lymphocyte, an antibody, or complement.
Drug Screening Assays, Antitumor
Methods of investigating the effectiveness of anticancer cytotoxic drugs and biologic inhibitors. These include in vitro cell-kill models and cytostatic dye exclusion tests as well as in vivo measurement of tumor growth parameters in laboratory animals.
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