Relationship of Bacillus amyloliquefaciens clades associated with strains DSM7T and FZB42: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on their discrimina

06:00 EDT 7th September 2010 | BioPortfolio

Summary of "Relationship of Bacillus amyloliquefaciens clades associated with strains DSM7T and FZB42: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on their discrimina"

The whole genome sequenced rhizobacterium FZB42 (Chen et al., 2007) and other plant-associated Bacillus strains either designated as Bacillus amyloliquefaciens or Bacillus subtilis are used commercially to promote growth and health of crop plants. Previous investigations revealed that the strains represent an own ecotype related to B. amyloliquefaciens, however its exact taxonomic position remains elusive (Reva et al., 2004). Here we have demonstrated ability to colonize Arabidopsis roots for a group of Bacillus strains, closely related to FZB42. According to their phenotypic traits the strains were similar to Bacillus amyloliquefaciens DSM 7T, but differed considerably in DNA sequences of the genes encoding 16S rRNA, gyrase subunit A (gyrA), and histidine kinase (cheA) from the type strain. Phylogenetic analysis performed with partial 16S rRNA, gyrA, and cheA sequences revealed that plant-associated Bacillus strains including FZB42 form a lineage, which can be discriminated from the cluster of strains closely related to B. amyloliquefaciens DSM 7T. DNA-DNA hybridization (DDH) performed with genomic DNAs from DSM 7T and FZB42 yielded 63.7 to 71.2 % homology. As complementary approach, we used several genomic methods, as direct whole genome comparison, digital DDH, and microarray-based comparative genomic hybridization (M-CGH). Plant-associated strains were discriminated from DSM 7T and B. subtilis type strain by their different potential to synthesize non-ribosomally lipopeptides and polyketides. According to the differences found in marker gene sequences and the whole genomes, we propose the two B. amyloliquefaciens subspecies, 'plantarum' for their plant-associated, and 'amyloliquefaciens', for their non-plant associated representatives. This is in line with results of DDH, MCGH, and the MALDI TOF mass spectrometry of cellular components which are justifying that both ecovars represent two different subspecies.


Bakteriengenetik, Institut für Biologie, Humboldt Universität Berlin;

Journal Details

This article was published in the following journal.

Name: International journal of systematic and evolutionary microbiology
ISSN: 1466-5026


DeepDyve research library

PubMed Articles [9509 Associated PubMed Articles listed on BioPortfolio]

Sequence comparison of phoR, gyrB, groEL, and cheA genes as phylogenetic markers for distinguishing Bacillus amyloliquefaciens and B. subtilis and for identifying Bacillus strain B29.

Given the close genetic relationship between Bacillus amyloliquefaciens and B. subtilis, distinguishing the two solely based on their physiological and biochemical characteristics and 16S rRNA sequenc...

Production of bioactive hydrolysate using protease, β-glucosidase and α-amylase of Bacillus spp. isolated from kinema.

The aim of this study was the production of soybean bioactive hydrolysate using Bacillus spp. isolated from kinema. Totally 251 bacteria isolated from kinema samples, collected at different time perio...

Isolation and characterization of a Bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential.

Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by Fusarium species, which has been shown to be associated with reproductive disorders in livestock, and to a lesser extent with hype...

Pan-genome analysis of Bacillus for microbiome profiling.

Recent advances in high-throughput sequencing technology allow for in-depth studies on microbial genomes and their communities. While multiple strains of the same species could display genomic variati...

Contribution of native phosphorous-solubilizing bacteria of acid soils on phosphorous acquisition in peanut (Arachis hypogaea L.).

The present investigation analyzes the in vitro P solubilization [Ca-P, Al-P, Fe(II)-P, and Fe(III)-P] efficiency of native PSB strains from acid soils of Odisha and exploitation of the same through b...

Clinical Trials [1723 Associated Clinical Trials listed on BioPortfolio]

Relationship of Staphylococcal Colonization to Infection

Inpatients will be prospectively have nares screened and MRSA strains collected. All clinical MRSA strains of patients will also be prospectively collected. A sensitive strain discriminati...

Clinical Effects of Bacillus Containing Oral Hygiene Products on Gingivitis: a Randomized Controlled Trial

Aim: Evaluating the clinical effects of a Bacillus subtilis, Bacillus megaterium and Bacillus pumulus containing toothpaste, mouthwash and toothbrush cleaner versus a placebo in patients w...

TEDDY: Spores of Bacillus Clausii in Acute Diarrhoea in Children

Primary objective: - To demonstrate the efficacy of a Bacillus clausii probiotic strain compared to placebo in children suffering from acute diarrhoea and treated for 7 days. ...

A Study to Evaluate OPTI-BIOME™ Bacillus Subtillis MB40 on Abdominal Discomfort, Gas and Bloating in a Healthy Population

The purpose of this study is to investigate the effects of OPTI-BIOME™ (Bacillus subtilis MB40), a probiotic supplement, on bloating, gas and abdominal discomfort symptoms in healthy adu...

Preventing Antibiotic-Associated DiarRhea Using Erceflora

To determine the effectiveness of the pre-biotic Bacillus clausii in preventing antibiotic associated diarrhea among hospitalized immunocompetent Filipino children.

Medical and Biotech [MESH] Definitions

A non-pathogenic species of Bacillus that occurs in soil as well as some fermented vegetables and FERMENTED DAIRY PRODUCTS. It produces BACTERIOCINS and ANTIFUNGAL AGENTS and is used in FOOD PRESERVATION as well as a PROBIOTIC.

A species of Bacillus that occurs in soil and is frequently found on bird feathers. Some strains cause FOODBORNE DISEASE, whereas other strains produce ANTIFUNGAL AGENTS and ANTIBACTERIAL AGENTS and function as PROBIOTICS. This species can also produce enzymes for industrial applications.

A species of Bacillus that occurs in soil and marine sediments. Many strains are alkalophilic and able to metabolize HEAVY METALS; it may therefore be a useful species for ENVIRONMENTAL BIODEGRADATION.

One of the Type II site-specific deoxyribonucleases (EC It recognizes and cleaves the sequence G/GATCC at the slash. BamHI is from Bacillus amyloliquefaciens N. Numerous isoschizomers have been identified. EC 3.1.21.-.

Bacteriocins elaborated by mutant strains of Bacillus megaterium. They are protein or protein-lipopolysaccharide complexes lethal to other strains of the same species.

Quick Search

DeepDyve research library

Relevant Topics

Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. During DNA sequencing, the bases of a small fragment of DNA are sequentially identified from signals emitted as each fragment is re-synthesized from a ...

Searches Linking to this Article