Track topics on Twitter Track topics that are important to you
The whole genome sequenced rhizobacterium FZB42 (Chen et al., 2007) and other plant-associated Bacillus strains either designated as Bacillus amyloliquefaciens or Bacillus subtilis are used commercially to promote growth and health of crop plants. Previous investigations revealed that the strains represent an own ecotype related to B. amyloliquefaciens, however its exact taxonomic position remains elusive (Reva et al., 2004). Here we have demonstrated ability to colonize Arabidopsis roots for a group of Bacillus strains, closely related to FZB42. According to their phenotypic traits the strains were similar to Bacillus amyloliquefaciens DSM 7T, but differed considerably in DNA sequences of the genes encoding 16S rRNA, gyrase subunit A (gyrA), and histidine kinase (cheA) from the type strain. Phylogenetic analysis performed with partial 16S rRNA, gyrA, and cheA sequences revealed that plant-associated Bacillus strains including FZB42 form a lineage, which can be discriminated from the cluster of strains closely related to B. amyloliquefaciens DSM 7T. DNA-DNA hybridization (DDH) performed with genomic DNAs from DSM 7T and FZB42 yielded 63.7 to 71.2 % homology. As complementary approach, we used several genomic methods, as direct whole genome comparison, digital DDH, and microarray-based comparative genomic hybridization (M-CGH). Plant-associated strains were discriminated from DSM 7T and B. subtilis type strain by their different potential to synthesize non-ribosomally lipopeptides and polyketides. According to the differences found in marker gene sequences and the whole genomes, we propose the two B. amyloliquefaciens subspecies, 'plantarum' for their plant-associated, and 'amyloliquefaciens', for their non-plant associated representatives. This is in line with results of DDH, MCGH, and the MALDI TOF mass spectrometry of cellular components which are justifying that both ecovars represent two different subspecies.
Bakteriengenetik, Institut für Biologie, Humboldt Universität Berlin;
This article was published in the following journal.
Name: International journal of systematic and evolutionary microbiology
To explore the influence of carbohydrates on the production of fengycin by Bacillus amyloliquefaciens fmb-60.
Bacillus species are attractive due to their potential use in the biological control of fungal diseases. Bacillus amyloliquefaciens strain BLB369, Bacillus subtilis strain BLB277, and Paenibacillus po...
Codonopsis pilosula (Franch.) Nannf. is a traditional Chinese herbal medicinal plant and a low-cost succedaneum for Panax ginseng and contains all various of bioactivity components. In this work, we f...
The neutral protease has high potential for industrial applications, and attempts to improve enzyme expression level have important application values. In the present study, a neutral protease-encodin...
Matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was used to establish a rapid, simple, and accurate method to differentiate among strains of Microcystis aer...
Aim: Evaluating the clinical effects of a Bacillus subtilis, Bacillus megaterium and Bacillus pumulus containing toothpaste, mouthwash and toothbrush cleaner versus a placebo in patients w...
Inpatients will be prospectively have nares screened and MRSA strains collected. All clinical MRSA strains of patients will also be prospectively collected. A sensitive strain discriminati...
Primary objective: - To demonstrate the efficacy of a Bacillus clausii probiotic strain compared to placebo in children suffering from acute diarrhoea and treated for 7 days. ...
To determine the effectiveness of the pre-biotic Bacillus clausii in preventing antibiotic associated diarrhea among hospitalized immunocompetent Filipino children.
To assess the effects of Bacillus clausii (Enterogermina®) on fecal microbial flora (using Polymerase Chain Reaction - Denaturing Gradient Gel Electrophoresis - PCR-DGGE - Method) in ant...
One of the Type II site-specific deoxyribonucleases (EC 220.127.116.11). It recognizes and cleaves the sequence G/GATCC at the slash. BamHI is from Bacillus amyloliquefaciens N. Numerous isoschizomers have been identified. EC 3.1.21.-.
Bacteriocins elaborated by mutant strains of Bacillus megaterium. They are protein or protein-lipopolysaccharide complexes lethal to other strains of the same species.
Viruses whose host is Bacillus. Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105.
A mixture of polymyxins B1 and B2, obtained from Bacillus polymyxa strains. They are basic polypeptides of about eight amino acids and have cationic detergent action on cell membranes. Polymyxin B is used for infections with gram-negative organisms, but may be neurotoxic and nephrotoxic.
A functional relationship between psychological phenomena of such nature that the presence of one tends to evoke the other; also, the process by which such a relationship is established.
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. During DNA sequencing, the bases of a small fragment of DNA are sequentially identified from signals emitted as each fragment is re-synthesized from a ...