Relationship of Bacillus amyloliquefaciens clades associated with strains DSM7T and FZB42: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on their discrimina
Summary of "Relationship of Bacillus amyloliquefaciens clades associated with strains DSM7T and FZB42: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on their discrimina"
The whole genome sequenced rhizobacterium FZB42 (Chen et al., 2007) and other plant-associated Bacillus strains either designated as Bacillus amyloliquefaciens or Bacillus subtilis are used commercially to promote growth and health of crop plants. Previous investigations revealed that the strains represent an own ecotype related to B. amyloliquefaciens, however its exact taxonomic position remains elusive (Reva et al., 2004). Here we have demonstrated ability to colonize Arabidopsis roots for a group of Bacillus strains, closely related to FZB42. According to their phenotypic traits the strains were similar to Bacillus amyloliquefaciens DSM 7T, but differed considerably in DNA sequences of the genes encoding 16S rRNA, gyrase subunit A (gyrA), and histidine kinase (cheA) from the type strain. Phylogenetic analysis performed with partial 16S rRNA, gyrA, and cheA sequences revealed that plant-associated Bacillus strains including FZB42 form a lineage, which can be discriminated from the cluster of strains closely related to B. amyloliquefaciens DSM 7T. DNA-DNA hybridization (DDH) performed with genomic DNAs from DSM 7T and FZB42 yielded 63.7 to 71.2 % homology. As complementary approach, we used several genomic methods, as direct whole genome comparison, digital DDH, and microarray-based comparative genomic hybridization (M-CGH). Plant-associated strains were discriminated from DSM 7T and B. subtilis type strain by their different potential to synthesize non-ribosomally lipopeptides and polyketides. According to the differences found in marker gene sequences and the whole genomes, we propose the two B. amyloliquefaciens subspecies, 'plantarum' for their plant-associated, and 'amyloliquefaciens', for their non-plant associated representatives. This is in line with results of DDH, MCGH, and the MALDI TOF mass spectrometry of cellular components which are justifying that both ecovars represent two different subspecies.
Bakteriengenetik, Institut für Biologie, Humboldt Universität Berlin;
This article was published in the following journal.
Name: International journal of systematic and evolutionary microbiology
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20817842
- DOI: http://dx.doi.org/10.1099/ijs.0.023267-0
Bacilli with fibrinolytic activities were isolated from traditionally-prepared Meju and some of these strains showed strong antifungal activities. One isolate, MJ1-4, showed the strongest antifungal a...
Here, we report the draft genome sequences of the type strains of three cellulolytic or hemicellulolytic alkaliphilic Bacillus species: Bacillus wakoensis, Bacillus akibai, and Bacillus hemicellulosil...
The phenotypic and genotypic diversity of the plant growth promoting Bacillus genus have been widely investigated in the rhizosphere of various agricultural crops. However, to our knowledge this is th...
Some naturally occurring Nif(+) and Nif(-)uperscript> strains of Bacillus polymyxa and Bacillus macerans were tested for DNA homology to the Klebsiella pneumoniae nif genes by Southern hybridization....
Bacillus strains JCM 19045, JCM 19046, and JCM 19047 are alkaliphiles that produce β-cyclodextrin from starch. They are related to Bacillus xiaoxiensis and Bacillus lehensis. The genome information f...
Inpatients will be prospectively have nares screened and MRSA strains collected. All clinical MRSA strains of patients will also be prospectively collected. A sensitive strain discriminati...
Primary objective: - To demonstrate the efficacy of a Bacillus clausii probiotic strain compared to placebo in children suffering from acute diarrhoea and treated for 7 days....
To determine the effectiveness of the pre-biotic Bacillus clausii in preventing antibiotic associated diarrhea among hospitalized immunocompetent Filipino children.
To assess the effects of Bacillus clausii (Enterogermina®) on fecal microbial flora (using Polymerase Chain Reaction - Denaturing Gradient Gel Electrophoresis - PCR-DGGE - Method) in ant...
Primary: - To demonstrate the efficacy of Enterogermina® in reducing the duration of acute diarrhea in children Secondary: - To evaluate the safety of Enterogerm...
Medical and Biotech [MESH] Definitions
One of the Type II site-specific deoxyribonucleases (EC 22.214.171.124). It recognizes and cleaves the sequence G/GATCC at the slash. BamHI is from Bacillus amyloliquefaciens N. Numerous isoschizomers have been identified. EC 3.1.21.-.
Bacteriocins elaborated by mutant strains of Bacillus megaterium. They are protein or protein-lipopolysaccharide complexes lethal to other strains of the same species.
Viruses whose host is Bacillus. Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105.
A mixture of polymyxins B1 and B2, obtained from Bacillus polymyxa strains. They are basic polypeptides of about eight amino acids and have cationic detergent action on cell membranes. Polymyxin B is used for infections with gram-negative organisms, but may be neurotoxic and nephrotoxic.
A functional relationship between psychological phenomena of such nature that the presence of one tends to evoke the other; also, the process by which such a relationship is established.