A comparative study of allergic contact dermatitis by patch test versus reflectance confocal laser microscopy, with nickel and cobalt.
Summary of "A comparative study of allergic contact dermatitis by patch test versus reflectance confocal laser microscopy, with nickel and cobalt."
Few studies have reported on the accuracy of reflectance confocal laser microscopy (RCLM) in observing allergic contact dermatitis in vivo. However, distinction of skin reactions from different reagents is not well understood. We sought to diagnose allergic contact dermatitis by RCLM images and compare with routine, visual patch test (PT) reading for 2 major allergen metals in Japan. The PT was performed on the upper back skin with 5% nickel sulfate (Ni) and 2% cobalt chloride (Co) in eight healthy volunteers and eleven patients. RCLM was used to calculate the thickness of the suprabasal epidermis after visual assessment of PT. Comparison of clinical scoring versus suprabasal epidermal thickness was observed. RCLM images of positive PT showed increased suprabasal epidermal thickness on day 2 (D2), and D3 for Co, whereas there was vesicle formation and an overall increase suprabasal epidermal thickness for Ni. In two of 3 doubtful positive PT to Co, RCLM images presented characteristics of irritant reactions; and one characteristic of a positive reaction. The frequency of Co doubtful-positive PT was higher than that of Ni. We found advantages in using RCLM for visualizing features of allergic contact dermatitis and found it a useful tool as an objective parameter in grading severity and types of PT reaction.
Affiliation
Journal Details
This article was published in the following journal.
Name: European journal of dermatology : EJD
ISSN: 1167-1122
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20822971
- DOI: http://dx.doi.org/10.1684/ejd.2010.1061
Medical and Biotech [MESH] Definitions
Dermatitis, Allergic Contact
A contact dermatitis due to allergic sensitization to various substances. These substances subsequently produce inflammatory reactions in the skin of those who have acquired hypersensitivity to them as a result of prior exposure.
Dermatitis, Contact
A type of acute or chronic skin reaction in which sensitivity is manifested by reactivity to materials or substances coming in contact with the skin. It may involve allergic or non-allergic mechanisms.
Dermatitis, Irritant
A non-allergic contact dermatitis caused by prolonged exposure to irritants and not explained by delayed hypersensitivity mechanisms.
Dermatitis, Toxicodendron
An allergic contact dermatitis caused by exposure to plants of the genus Toxicodendron (formerly Rhus). These include poison ivy, poison oak, and poison sumac, all plants that contain the substance urushiol, a potent skin sensitizing agent. (From Dorland, 27th ed)
Tulipa
A plant genus of the family LILIACEAE. Members contain tuliposides and tulipalins and have been associated with allergic contact dermatitis in florists.
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