Flow-injection determination of hydrogen peroxide based on fluorescence quenching of chromotropic acid catalyzed with Fe(II).
Summary of "Flow-injection determination of hydrogen peroxide based on fluorescence quenching of chromotropic acid catalyzed with Fe(II)."
Flow-injection analysis system (FIA system), which was based on Fe(II)-catalyzed oxidation of chromotropic acid with hydrogen peroxide, was developed for the determination of hydrogen peroxide. The chromotropic acid has a fluorescence measured at lambda(em)=440 nm (emission wavelength) with lambda(ex)=235 nm (excitation wavelength), and the fluorescence intensity at lambda(em)=440 nm quietly decreased in the presence of hydrogen peroxide and Fe(II), which was caused by Fe(II)-catalyzed oxidation of chromotropic acid with hydrogen peroxide. By measuring the difference of fluorescence intensity, hydrogen peroxide (1.0 x 10(-8)-1.0 x 10(-3) mol L(-1)) could be determined by the proposed FIA system, whose analytical throughput was 40 samples h(-1). The relative standard deviation (RSD) was 1.03% (n=10) for 4.0 x 10(-8) mol L(-1) hydrogen peroxide. The proposed FIA technique could be applied to the determination of hydrogen peroxide in rain water samples.
Affiliation
Analytical and Testing Center of Yanbian University, Gongyuan 977, Yanji 133002, China.
Journal Details
This article was published in the following journal.
Name: Talanta
ISSN: 1873-3573
Pages: 1225-9
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20801322
- DOI: http://dx.doi.org/10.1016/j.talanta.2010.06.039
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The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.
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