Supplementation of Fibrin Gels with Sodium Chloride Enhances Physical Properties and Ensuing Osteogenic Response.
Summary of "Supplementation of Fibrin Gels with Sodium Chloride Enhances Physical Properties and Ensuing Osteogenic Response."
Modifying the relative concentrations of fibrinogen and thrombin can control the physical properties of fibrin gels, while the viability of associated cells has been linked to the gel's final network structure. We hypothesized that increasing the gel ionic strength during fabrication through supplementation with sodium chloride (NaCl) would provide an improved approach for tailoring the physical properties of fibrin gels and maintaining the viability and osteogenic potential of entrapped cells. Fibrin gels were formed by mixing fibrinogen, thrombin, and calcium chloride with varying masses of NaCl (0-4.40% w/v), and the osteogenic potential of entrapped human mesenchymal stem cells (MSCs) was examined over 14 days. Physical properties including gelation time, compressive modulus, and fiber diameter were dependent upon NaCl content, with gels containing 2.60% NaCl possessing compressive moduli 3-fold higher than gels without NaCl. Alkaline phosphatase activity was highest for MSCs entrapped in gels containing 2.15-2.60% NaCl after 14 days, and all gels exhibited increased calcium incorporation over the culture period. These data confirm that varying the salt concentration of the pre-gel solution can modulate the material properties of fibrin constructs without additional fibrinogen or thrombin, thereby offering a new approach for generating improved cell transplantation vehicles for use in bone tissue regeneration.
Affiliation
Department of Biomedical Engineering, UC Davis, Davis, CA 95616.
Journal Details
This article was published in the following journal.
Name: Acta biomaterialia
ISSN: 1878-7568
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20837168
- DOI: http://dx.doi.org/10.1016/j.actbio.2010.09.007
Medical and Biotech [MESH] Definitions
Sodium-potassium-chloride Symporters
A subclass of symporters that specifically transport SODIUM CHLORIDE and POTASSIUM CHLORIDE across cellular membranes in a tightly coupled process.
Sodium Chloride Symporter Inhibitors
Agents that inhibit SODIUM CHLORIDE SYMPORTERS. They act as DIURETICS. Excess use is associated with HYPOKALEMIA.
Sodium Chloride, Dietary
Sodium chloride used in foods.
Sodium, Dietary
Sodium or sodium compounds used in foods or as a food. The most frequently used compounds are sodium chloride or sodium glutamate.
Sodium Potassium Chloride Symporter Inhibitors
Agents that inhibit SODIUM-POTASSIUM-CHLORIDE SYMPORTERS which are concentrated in the thick ascending limb at the junction of the LOOP OF HENLE and KIDNEY TUBULES, DISTAL. They act as DIURETICS. Excess use is associated with HYPOKALEMIA and HYPERGLYCEMIA.
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