DNA hybridization induced selective encapsulation of small dye molecules in dendrimer based microcapsules.
Summary of "DNA hybridization induced selective encapsulation of small dye molecules in dendrimer based microcapsules."
A novel approach for selectively encapsulating Cy5 dye molecules via DNA hybridization in cationic phosphorus dendrimers (G(4)(+))/polystyrenesulfonate (PSS) microcapsules has been developed. The hybridization between Cy5 labeled single strand DNA (ssDNA) targets and complementary ssDNA probes was performed in the microcapsules. During hybridization, the ssDNA targets acted as 'vehicles' and Cy5 dye molecules as 'cargos', which led to the selective encapsulation of the Cy5 molecules inside the capsules. The approach may be applied for many small molecules, which are usually difficult to be encapsulated due to their diffusion properties in microcapsules.
Affiliation
State Key Lab of Metal matrix Composites, School of Materials Science & Engineering, Shanghai Jiao Tong University, 800 DongChuan Road, Shanghai, 200240, China. clfeng@sjtu.edu.cn.
Journal Details
This article was published in the following journal.
Name: The Analyst
ISSN: 1364-5528
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20844779
- DOI: http://dx.doi.org/10.1039/c0an00334d
Medical and Biotech [MESH] Definitions
Molecular Probes
A group of atoms or molecules attached to other molecules or cellular structures and used in studying the properties of these molecules and structures. Radioactive DNA or RNA sequences are used in MOLECULAR GENETICS to detect the presence of a complementary sequence by NUCLEIC ACID HYBRIDIZATION.
Breeding
The production of offspring by selective mating or HYBRIDIZATION, GENETIC in animals or plants.
In Situ Hybridization, Fluorescence
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
Denaturing Gradient Gel Electrophoresis
Electrophoresis in which various denaturant gradients are used to induce nucleic acids to melt at various stages resulting in separation of molecules based on small sequence differences including SNPs. The denaturants used include heat, formamide, and urea.
Capillary Permeability
The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.
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