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Purpose: CD36 is a Class B scavenger receptor that is constitutively expressed in the corneal epithelium and has been implicated in many homeostatic functions, including the homeostasis of the epidermal barrier. The aim of this study is to determine (i) whether CD36 is required for the maintenance of the corneal epithelial barrier to infection, and (ii) whether CD36 deficient mice present with an increased susceptibility to bacterial keratitis. Methods: The corneas of CD36-/-, TSP-1-/-, TLR2-/-, and C57BL/6 WT mice were screened via slit lamp microscopy or ex vivo analysis. The epithelial tight junctions and mucin layer were assessed via LC-biotin and Rose Bengal staining, respectively. Bacterial quantification was performed on corneal buttons and S. aureus conjugated to FITC was used to study bacterial binding. Results: CD36-/- mice develop spontaneous corneal defects that increased in frequency and severity with age. The mild corneal defects were characterized by (i) a disruption in epithelial tight junctions and the mucin layer, (ii) an infiltrate of macrophages and (iii) increased bacterial binding. Bacterial quantification revealed high levels of S. xylosus in the corneas of CD36-/- mice with severe defects, but not in WT controls. Conclusion: CD36-/- mice develop spontaneous bacterial keratitis independent of TLR2 and TSP-1. We conclude that CD36 is a critical component of the corneal epithelial barrier and in the absence of CD36 the barrier breaks down allowing bacteria to bind to the corneal epithelium, resulting in spontaneous keratitis. This is the first report of spontaneous bacterial keratitis in mice.
Department of Ophthalmology, Harvard Medical School, Schepens Eye Research Institute, Boston, United States.
This article was published in the following journal.
Name: Investigative ophthalmology & visual science
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Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.
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