Electrochemical Immunosensor for Simultaneous Detection of Dual Cardiac Markers Based on a Poly(Dimethylsiloxane) (PDMS)-Gold Nanoparticles Composite Microfluidic Chip: A Proof of Principle.
Summary of "Electrochemical Immunosensor for Simultaneous Detection of Dual Cardiac Markers Based on a Poly(Dimethylsiloxane) (PDMS)-Gold Nanoparticles Composite Microfluidic Chip: A Proof of Principle."
BACKGROUND:
The emergence of microfluidic immunosensors has provided a promising tool for improving clinical diagnoses. We developed an electrochemical immunoassay for the simultaneous detection of cardiac troponin I (cTnI) and C-reactive protein (CRP), based on microfluidic chips.
METHODS:
The quantitative methodology was based on ELISA in poly(dimethylsiloxane)-gold nanoparticle composite microreactors. CdTe and ZnSe quantum dots were bioconjugated with antibodies for sandwich immunoassay. After the CdTe and ZnSe quantum dots were dissolved, Cd(2+) and Zn(2+) were detected by square-wave anodic stripping voltammetry to enable the quantification of the two biomarkers. The two biomarkers were measured in 20 human serum samples using the proposed method and commercially available methods.
RESULTS:
This immunosensor allowed simultaneous detection of serum cTnI and CRP. The linear range of this assay was between 0.01 and 50 μg/L and 0.5 and 200 μg/L, with the detection limits of approximately 5 amol and approximately 307 amol in 30-μL samples corresponding to cTnI and CRP, respectively. Slopes close to 1 and the correlation coefficient over 0.99 were obtained for both analytes.
CONCLUSIONS:
This strategy demonstrates a proof of principle for the successful integration of microfluidics with electrochemistry that can potentially provide an alternative to protein detection in the clinical laboratory.
Affiliation
Key Lab of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China.
Journal Details
This article was published in the following journal.
Name: Clinical chemistry
ISSN: 1530-8561
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20852134
- DOI: http://dx.doi.org/10.1373/clinchem.2010.147256
Medical and Biotech [MESH] Definitions
Multiphasic Screening
The simultaneous use of multiple laboratory procedures for the detection of various diseases. These are usually performed on groups of people.
Ventricular Pressure
The pressure within a CARDIAC VENTRICLE. Ventricular pressure waveforms can be measured in the beating heart by catheterization or estimated using imaging techniques (e.g., DOPPLER ECHOCARDIOGRAPHY). The information is useful in evaluating the function of the MYOCARDIUM; CARDIAC VALVES; and PERICARDIUM, particularly with simultaneous measurement of other (e.g., aortic or atrial) pressures.
Substance Abuse Detection
Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.
Heart-lung Transplantation
The simultaneous, or near simultaneous, transference of heart and lungs from one human or animal to another.
Polarography
An electrochemical technique for measuring the current that flows in solution as a function of an applied voltage. The observed polarographic wave, resulting from the electrochemical response, depends on the way voltage is applied (linear sweep or differential pulse) and the type of electrode used. Usually a mercury drop electrode is used.
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