Pediatric reference intervals for lymphocyte vitamin C (ascorbic acid).
Summary of "Pediatric reference intervals for lymphocyte vitamin C (ascorbic acid)."
OBJECTIVE:
To establish pediatric reference intervals for lymphocyte vitamin C. STUDY
DESIGN:
This was a prospective study of 194 well children aged 0-7years old of mixed ethnicity who had blood drawn for the purpose of this study. Blood was collected during elective surgery under general anesthesia and lymphocytes isolated and stored as frozen ascorbic acid lymphoyte lysates for later HPLC analysis by previously described methodology. Reference intervals were established according to the Clinical and Laboratory Standards Institute (CLSI) and the International Federation of Clinical Chemistry (IFCC) guidelines (C28-A3). Horn-Pesce robust method was used to estimate the 95% confidence interval and 95% reference interval.
RESULTS:
Reference intervals were independent of age or gender and shown to be 12.9 - 52.8μg/10(8) cells (lymphocytes).
CONCLUSION:
We have defined pediatric reference ranges for lymphocyte vitamin C in healthy, fasted children at an age group (0-7years). The new reference interval can now be used to more reliably explore possible implications of variation of vitamin C levels on bleeding and other clinical signs.
Affiliation
Pediatric Ophthalmology and Ocular Genetics, Wills Eye Institute, Philadelphia, USA.
Journal Details
This article was published in the following journal.
Name: Clinical biochemistry
ISSN: 1873-2933
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20858473
- DOI: http://dx.doi.org/10.1016/j.clinbiochem.2010.09.008
Medical and Biotech [MESH] Definitions
Ascorbic Acid
A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.
Ascorbic Acid Deficiency
A condition due to a dietary deficiency of ascorbic acid (vitamin C), characterized by malaise, lethargy, and weakness. As the disease progresses, joints, muscles, and subcutaneous tissues may become the sites of hemorrhage. Ascorbic acid deficiency frequently develops into SCURVY in young children fed unsupplemented cow's milk exclusively during their first year. It develops also commonly in chronic alcoholism. (Cecil Textbook of Medicine, 19th ed, p1177)
Scurvy
An acquired blood vessel disorder caused by severe deficiency of vitamin C (ASCORBIC ACID) in the diet leading to defective collagen formation in small blood vessels. Scurvy is characterized by bleeding in any tissue, weakness, ANEMIA, spongy gums, and a brawny induration of the muscles of the calves and legs.
2,3-diketogulonic Acid
Metabolite of ASCORBIC ACID and the oxidized form of the lactone DEHYDROASCORBIC ACID.
Vitamin K
A lipid cofactor that is required for normal blood clotting. Several forms of vitamin K have been identified: VITAMIN K 1 (phytomenadione) derived from plants, VITAMIN K 2 (menaquinone) from bacteria, and synthetic naphthoquinone provitamins, VITAMIN K 3 (menadione). Vitamin K 3 provitamins, after being alkylated in vivo, exhibit the antifibrinolytic activity of vitamin K. Green leafy vegetables, liver, cheese, butter, and egg yolk are good sources of vitamin K.
PubMed Articles
Influence of Trace Elements on Stabilization of Aqueous Solutions of Ascorbic Acid.
Together with vitamin C, zinc, selenium, manganese, and magnesium play a vital role in the preservation of organs scheduled for transplantation. In the present study, it is shown that addition of 1 m...
L-Ascorbic acid (vitamin C) supplementation to optimize health and reproduction in cattle.
Cattle can synthesize L-ascorbic acid (or Vitamin C) from either D-glucose or D-galactose through glucuronic acid pathway in the liver. L-Ascorbic acid present in cattle diet is almost totally destroy...
Photostability and Interaction of Ascorbic Acid in Cream Formulations.
The kinetics of photolysis of ascorbic acid in cream formulations on UV irradiation has been studied using a specific spectrophotometric method with a reproducibility of ±5%. The apparent first-order...
By controlling the reflux time and the quantity of the shell materials, different sizes of thioglycollic acid (TGA) modified CdTe/CdSe core/shell quantum dots were synthesized in aqueous solution. Thi...
Ascorbic acid status of female camels during different phases of reproduction.
There is suggestive evidence that a low status of ascorbic acid in camels enhances their risk for infectious diseases. This study was carried out to disclose the role of reproduction, if any, in affec...
Clinical Trials
High Dose Ascorbic Acid Treatment of CMT1A
This study will look at the impact of ascorbic acid (Vitamin C) on the progression of disease in people with CMT1A as compared to volunteers receiving a placebo. This study will assess wh...
Study of High Dose Intravenous (IV) Ascorbic Acid in Measurable Solid Tumor Disease
The study is designed to determine if high doses of intravenous ascorbic acid (vitamin C) can be effective in managing solid tumor diseases. Secondary goals are determination of any pallia...
Study of High-Dose Intravenous (IV) Vitamin C Treatment in Patients With Solid Tumors
The primary purpose of this study is to evaluate the safety and tolerability of vitamin C (ascorbic acid) given by injection into the vein. The second and third purpose of conducting this...
Impact of Ascorbic Acid on Post-Cardiothoracic Surgery Inflammation
The purpose of this study is to see if ascorbic acid (Vitamin-C) therapy will reduce inflammation following heart surgery.
Study of Oral Arsenic Trioxide With or Without Ascorbic Acid in Adults With Myelofibrosis
This study determine the highest safe dose of arsenic trioxide with or without ascorbic acid and if the investigational drug arsenic trioxide along with ascorbic acid (vitamin C) is effect...
