Pediatric reference intervals for lymphocyte vitamin C (ascorbic acid).
Summary of "Pediatric reference intervals for lymphocyte vitamin C (ascorbic acid)."
To establish pediatric reference intervals for lymphocyte vitamin C. STUDY
This was a prospective study of 194 well children aged 0-7years old of mixed ethnicity who had blood drawn for the purpose of this study. Blood was collected during elective surgery under general anesthesia and lymphocytes isolated and stored as frozen ascorbic acid lymphoyte lysates for later HPLC analysis by previously described methodology. Reference intervals were established according to the Clinical and Laboratory Standards Institute (CLSI) and the International Federation of Clinical Chemistry (IFCC) guidelines (C28-A3). Horn-Pesce robust method was used to estimate the 95% confidence interval and 95% reference interval.
Reference intervals were independent of age or gender and shown to be 12.9 - 52.8μg/10(8) cells (lymphocytes).
We have defined pediatric reference ranges for lymphocyte vitamin C in healthy, fasted children at an age group (0-7years). The new reference interval can now be used to more reliably explore possible implications of variation of vitamin C levels on bleeding and other clinical signs.
Pediatric Ophthalmology and Ocular Genetics, Wills Eye Institute, Philadelphia, USA.
This article was published in the following journal.
Name: Clinical biochemistry
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20858473
- DOI: http://dx.doi.org/10.1016/j.clinbiochem.2010.09.008
Medical and Biotech [MESH] Definitions
A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.
Ascorbic Acid Deficiency
A condition due to a dietary deficiency of ascorbic acid (vitamin C), characterized by malaise, lethargy, and weakness. As the disease progresses, joints, muscles, and subcutaneous tissues may become the sites of hemorrhage. Ascorbic acid deficiency frequently develops into SCURVY in young children fed unsupplemented cow's milk exclusively during their first year. It develops also commonly in chronic alcoholism. (Cecil Textbook of Medicine, 19th ed, p1177)
An acquired blood vessel disorder caused by severe deficiency of vitamin C (ASCORBIC ACID) in the diet leading to defective collagen formation in small blood vessels. Scurvy is characterized by bleeding in any tissue, weakness, ANEMIA, spongy gums, and a brawny induration of the muscles of the calves and legs.
Metabolite of ASCORBIC ACID and the oxidized form of the lactone DEHYDROASCORBIC ACID.
A lipid cofactor that is required for normal blood clotting. Several forms of vitamin K have been identified: VITAMIN K 1 (phytomenadione) derived from plants, VITAMIN K 2 (menaquinone) from bacteria, and synthetic naphthoquinone provitamins, VITAMIN K 3 (menadione). Vitamin K 3 provitamins, after being alkylated in vivo, exhibit the antifibrinolytic activity of vitamin K. Green leafy vegetables, liver, cheese, butter, and egg yolk are good sources of vitamin K.
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