Spectrophotometric determination of penicillins in pure and pharmaceutical formulations using Folin-Ciocalteu reagent.
Summary of "Spectrophotometric determination of penicillins in pure and pharmaceutical formulations using Folin-Ciocalteu reagent."
A new combination of time, temperature, and alkali is described for the spectrophotometric determination of amoxicillin and ampicillin using Folin-Ciocalteu reagent. The method is based on the development of blue-coloured product due to the reduction of tungstate and/or molybdate in Folin-Ciocalteu reagent by amoxicillin and ampicillin in alkaline medium. The chromogenic reaction has λ(max) at 720 and 740 nm with molar absorptivity 1.6295 × 10(4) and 0.1085 × 10(4) l mol(-1) cm(-1) in the Beer's Law range 2-10 µg mL(-1) and 10-70 µg mL(-1) for amoxicillin and ampicillin, respectively. The method is reproducible, quick, inexpensive, and particularly helpful in determining the drug content in commercial dosage forms. Copyright © 2010 John Wiley & Sons, Ltd.
Affiliation
Department of Chemistry, Harcourt Butler Technological Institute, Kanpur-208 002, India.
Journal Details
This article was published in the following journal.
Name: Drug testing and analysis
ISSN: 1942-7611
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20865758
- DOI: http://dx.doi.org/10.1002/dta.157
Medical and Biotech [MESH] Definitions
Audiometry, Pure-tone
Measurement of hearing based on the use of pure tones of various frequencies and intensities as auditory stimuli.
Insurance Claim Review
Review of claims by insurance companies to determine liability and amount of payment for various services. The review may also include determination of eligibility of the claimant or beneficiary or of the provider of the benefit; determination that the benefit is covered or not payable under another policy; or determination that the service was necessary and of reasonable cost and quality.
Sasa
A plant genus of the family POACEAE. Folin is the water-soluble extract from Sasa albomarginata. Sasa kurinensis is an ingredient of Sho-ju-sen, a Japanese herbal medicine.
Spectrophotometry, Atomic
Spectrophotometric techniques in which the samples are examined in the form of atoms based on their unique energy levels for ELECTRONS. They are used to analyze TRACE ELEMENTS, such as ALUMINUM; ARSENIC; BERYLLIUM; CALCIUM; COPPER; IRON; LEAD; and LITHIUM.
Dicloxacillin
One of the PENICILLINS which is resistant to PENICILLINASE.
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