PubMed Journal Database | Cell biology international 
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Showing PubMed Articles 1–25 of 185 from Cell biology international
Accumulating evidence demonstrates that chronic inflammation plays an important role in heart hypertrophy and cardiac diseases. However, the fine-tuning of cellular and molecular mechanisms that connect inflammatory process and cardiac diseases is still under investigation. Many reports have demonstrated that the overexpression of the cyclooxygenase-2 (COX-2), a key enzyme in the conversion of arachidonic acid to prostaglandins and other prostanoids, is correlated with inflammatory processes. Increased leve...
SiRNA-targeted carboxypeptidase D inhibits hepatocellular carcinoma growth.
Carboxypeptidase D (CPD), a membrane-bound metallocarboxypeptidase that functions as a docking receptor for duck hepatitis B virus, is frequently overexpressed in human cancers. We have explored its expression pattern, clinical significance, and biological function of CPD in hepatocellular carcinoma (HCC). CPD expression was markedly elevated in HCCs relative to adjacent non-tumor liver tissues, as determined by quantitative real-time polymerase chain reaction (qPCR) and Western blot analysis. Immunohistoch...
New Strategies in Cord Blood Cells Transplantation.
For patients lacking an HLA-matched donor, umbilical cord blood (UCB) is an ideal, alternative source of hematopoietic stem cells (HSCs) for transplantation purposes. UCB has many advantages over bone marrow or peripheral blood taken from volunteer donors. UCB is also an important source of other stem cells, including endothelial progenitors, mesenchymal stem cells (MSCs), very small embryonic/epiblast-like (VSEL) stem cells, and unrestricted somatic stem cells (USSC), which are potentially suitable for reg...
FoxO1 and C/EBPβ are important transcription factors during adipogenic differentiation; however, the potential mechanisms of their inter-regulation in adipogenesis remain unknown. We found that FoxO1 and C/EBPβ are abundant in adipose tissues of 3- and 180-day pigs at the mRNA and protein levels. During porcine preadipocyte differentiation, C/EBPβ expression increases to the peak at day 2 and then decreases. In contrast, FoxO1 is lowest on day 2 and gradually increases. Knockdown of FoxO1 or C/EBPβ with...
3D Arrays for High Throughput Assay of Cell Migration and Electrotaxis.
Cell behaviorin 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behavior in 3D, it is necessary to develop high throughput techniques to study behavior in 3D. We report on a 3D array on slide and have adapted this to our electrotaxis chamber, thereby offering a novel approach to quantify cellular responses to electric fields (EFs) in 3D conditions, in different matrices, with diff...
To observe the effects of miR-125b on the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into neuron-like cells, rat BMSCs were isolated and transfected with Syn-rno-miR-125b* miScript miRNA Mimic (Mimic + BME group), with anti-rno-miR-125b* miScript miRNA Inhibitor (Inhibitor + BME group) and BME control was set up without transfection. Blank controls without transfection and induction by BME was also set up. BMSCs of 3 groups including Mimic + BME group, Inhibitor + BME...
Cervical squamous carcinoma is a highly invasive tumour that has a great capacity to metastasise. Extracellular matrix metalloproteinase inducer (EMMPRIN or CD147), a member of the immunoglobulin superfamily, is a widely distributed cell surface glycoprotein. It is highly expressed on malignant tumour cell surfaces, including human cervical squamous carcinoma. It also plays a critical role in the invasive and metastatic activity of malignant cells by stimulating the expression of matrix metalloproteinases (...
Notch2, a surface marker in cell lines, is used to isolate, identify and localize pancreatic cancer stem-like cells and is a target for therapy of these cells. Sphere formation was induced in Panc-1 and Bxpc-3 pancreatic cancer cell lines, and Notch2(+) cells were separated from Bxpc-3 and Panc-1 cell lines by magnetic activated cell sorting (MACS). Expression of stem cell-related markers, OCT4, Nanog and PDX1, were measured by immunofluoresent (IF) staining. Expression of Notch2 was also determined immunoh...
Inhibition of ATP-induced calcium influx by homocysteine in human umbilical vein endothelial cells.
Mechanisms involved in the association between hyperhomocysteinemia and vascular occlusive diseases remain unclear. Homocysteine (Hcy) may disturb calcium (Ca(2+) ) cytosolic regulation in endothelial cells, a process that can directly affect the synthesis of vasoactive substances, such as nitric oxide (NO). We have investigated the effect of acute and chronic incubation with high concentrations of Hcy (100 and 500 μmoles/L) on the changes in the intracellular Ca(2+) concentration ([Ca(2+) ](i) ) induced...
Expression of miR-34c is significantly reduced in human endometrial cancer cells and miR-34c regulates multiple genes associated with cancer cell proliferation, apoptosis and invasiveness. Thus, miR-34c can theoretically become a target that enhances chemotherapy. Upregulation of miR-34c to enhance the chemotherapeutic effect of cisplatin (DDP) has not been studied in human endometrial cancer cells before. In an in vitro study, the human endometrial cancer cell line, Ishikawa, was treated with DDP and miR-3...
Induction of HO-1 protein can have both beneficial and detrimental effects for cells, including regulating proliferation and apoptosis of several tumors. We have investigated the regulation of HO-1, p38MAPK and mTOR in the context of proliferation, cell cycle events and apoptosis of esophageal squamous cell carcinoma cells. Real-time PCR and western blots were used to determine the expression levels of HO-1, p38MAPK, p-p38MAPK and p-mTOR. MTT assays were used to measure proliferation, FACS for cell cycle ev...
Integrin alpha v beta 6 contributes to maintaining corneal epithelial barrier function.
The corneal epithelial barrier dysfunction is associated with a number of disorders of the cornea. This study aims to investigate the role of integrin alpha v beta 6 (avb6) in maintaining the corneal epithelial barrier function. In this study, the association of avb6 and endosome/lysosome in the human corneal epithelial cell line, HCE cells, was observed with immunocytochemistry. The corneal epithelial barrier function was assessed with HCE monolayers in Transwells. The results showed that avb6 was observed...
Autologous fat tissue has been used as a potential filler for soft-tissue defects, despite unpredictable clinical outcomes and low graft survival. Co-transplantation of adipose-derived stem cells (ASCs) is an alternative therapeutic approach to effectively enhance the survival and quality of transplanted fat tissue by increasing neovascularization. Nevertheless, the mechanisms by which ASCs exerted their angiogenic effects remain obscure. ASCs can secrete several angiogenic growth factors, e.g. vascular end...
We have investigates the effect of visible light on animal cells using light-emitting diodes (LEDs) to emit monochromatic visible light (red, yellow, green and blue light). To explore the relevant mechanism of apoptosis, we assessed the intracellular superoxide anion production and mitochondrial membrane potential (ΔΨm) of B16F1 and B16F10 murine melanoma cells after monochromatic light irradiation. Blue light caused ΔΨm depolarization subsequent to elevation of intracellular superoxide production. Howe...
Nitric oxide protects endothelium from cadmium mediated leakiness.
Cadmium targets the vascular endothelium causing endothelial dysfunction and leakiness of endothelial barrier. Nitric oxide plays a major role in mediating endothelial functions including angiogenesis, migration and permeability. The present study investigates the nitric oxide effects on cadmium induced endothelial leakiness. Results of ex vivo and in vitro permeability assays showed that even a sub-lethal dose of cadmium chloride (1 µM) was sufficient to induce leakiness of endothelial cells. Cadmium dr...
Regulation of expression of HGF in BM-MSCs by baculovirus-mediated transduction.
Bone marrow-derived mesenchymal stem cells (BM-MSCs) transplantation is widely adopted for the curing of osteonecrosis of femoral head (ONFH) in recent years. Furthermore, it is known that introducing of hepatocyte growth factor (HGF) into BM-MSCs will greatly improve the therapeutic effect of stem-cell therapy owing to the great angiogenic and anti-fibrotic capabilities of HGF. However, continuing overexpression of HGF in vivo may cause sarcomas, such as Kaposi's sarcoma. Aiming at enhancing the therapeuti...
Better pregnancy outcomes can be obtained by human mature oocyte vitrification, but many problems remain to be resolved in human mature oocyte vitrification. Since mature oocyte development possesses its own maturity cycle, there should be the optimal timing for mature oocyte vitrification. The purpose of this study was to observe the effects of frozen timing on the spindle density, the angle between the polar body and spindle, and embryo development of intracytoplasmic sperm injection (ICSI) in vitrified m...
Insulin Regulated Expression of Adiponectin Receptors in Muscle and Fat Cells.
Adiponectin (Adp), an adipocyte secreted hormone, exerts its effect via its specific receptors, adiponectin receptors (AdipoR1 and AdipoR2), in insulin sensitive cells in muscle, liver, and adipose tissues and plays an important role in lipid and glucose metabolism. The aim of the current study was to investigate the effect of insulin on AdipoRs expression in muscle and fat cells. Differentiated fat (3T3-L1), skeletal muscle (L6), and vascular smooth muscle (PAC1) cells were serum starved and exposed to 100...
Mesenchymal stem cells restore CCl4-induced liver injury by an antioxidative process.
We studied bone marrow (BM)-derived mesenchymal stem cells (MSCs) for the treatment of liver injury. We hypothesized that MSC-mediated resolution of liver injury could occurred through an antioxidative process. After being injected with carbon tetrachloride (CCl4), mice were injected with syngenic BM-derived MSCs or normal saline. Oxidative stress activity of the MSCs was determined by analysis of reactive oxygen species (ROS) and superoxide dismutase (SOD) activity. In addition, cytoprotective genes of liv...
Previous cytogenetic studies in triatomines have described the occurrence of holokinetic chromosomes, heterochromatin distribution and the location of rDNA sites, but few aspects of nuclear organization in this group have been discussed. In this study, we focused on ultrastructural and cytogenetic features and differences in cystic cells of seminiferous tubules between five species of Triatoma. We noted that cystic cells show evidence of polyploidy events and that heterochromatic blocks appear predominantly...
Platelet-rich plasma (PRP)-derived growth factors are a new application of tissue engineering and a developing area for researchers and clinicians. This study aimed to evaluate the effects of PRP-derived growth factors on the proliferation and osteogenic differentiation of rat muscle satellite cells (rMSCs) and to construct a novel tissue engineering bone composed of PRP-derived growth factors and rMSCs. PRP were created by a freeze-thaw process. RMSCs were isolated from rat masticatory muscle using serial...
BMP4 can generate primordial germ cells from bone marrow-derived pluripotent stem cells.
The evidence of germ cell derivation from embryonic and somatic stem cells provides an in vitro model for the study of germ cell development, the associated epigenetic modification and mammalian gametogenesis. More importantly, in vitro derived gametes also represent a potential strategy for treating infertility. In mammals, male and female gametes, oocyte and sperm, are derived from a specific cell population, primordial germ cells (PGCs), that segregate early in embryogenesis. In the present study we trie...
Adipose derived mesenchymal stem cells are candidate adult stem cells for regenerative medicine. Notch signaling has been reported to participate in the differentiation of a heterogeneous adipose derived mesenchymal stem cell population. Here, human adipose tissue derived single-cell clones were isolated using a cloning ring technique and characterized for their stem cell characteristics. In addition, the role of Notch signaling on the differentiation capacity of these adipose derived single-cell-clones was...
Endogenous and induced angiogenic characteristics of human chorion-derived stem cells.
Cell-based therapy using stem cells has emerged as one of the pro-angiogenic methods to enhance blood vessel growth and sprouting in ischemic conditions. However this mode of therapy is still in its early stages of development with many more questions needed to be answered. This study investigated the endogenous and induced angiogenic characteristics of human chorion-derived stem cell (hCDSC) using quantitative Polymerase Chain Reaction method, immunocytochemistry and fibrin-matrigel migration assay. The re...
The inducible Hsp70 family members (iHsp70, Hsp72 and Hsp70) are highly conserved proteins that act as molecular chaperones and promote cell survival during various forms of stress. Our data indicate that cultured adult rabbit myoblasts do not express iHsp70 under normal growth conditions, although an increased expression of that particular protein was detectable 0.5 - 72 h following a 42°C heat shock for 15-60 min. The intracellular iHsp70 level reached a maximum 8 h after onset of the heat shock which co...
