PubMed Journals Articles About "Biotech" 
Biotech PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Biotech articles that have been published worldwide.
More Information about "Biotech" on BioPortfolio
We have published hundreds of Biotech news stories on BioPortfolio along with dozens of Biotech Clinical Trials and PubMed Articles about Biotech for you to read. In addition to the medical data, news and clinical trials, BioPortfolio also has a large collection of Biotech Companies in our database. You can also find out about relevant Biotech Drugs and Medications on this site too.
Showing "Biotech" PubMed Articles 1–25 of 167
Cuttlefish bones (CBs) have emerged as attractive biomaterials because of their porous structure and components that can be converted into hydroxyapatite (HAp) via a hydrothermal reaction. However, their brittleness and low strength restrict their application in bone tissue engineering. Therefore, to improve the compressive strength of the scaffold following hydrothermal conversion to a HAp form of CB (CB-HAp), the scaffold was coated using a polycaprolactone (PCL) polymer at various concentrations. In this...
Background: Brown adipose tissue plays a pivotal role in mammal metabolism and thermogenesis. It has a great therapeutic potential in several metabolic disorders such as obesity and diabetes. Mesenchymal stem cells (MSCs) are suitable candidates for brown adipose tissue formation de novo. Pparγ2 and C/ebpα are nucleic receptors known to mediate adipogenic differentiation. We hypothesized that overexpression of the Pparγ2 and C/ebpα genes in MSCs would lead to the formation of adipose tissue. Materials &...
Porphyromonas gingivalis, a major etiological agent of chronic periodontitis, acquires haem from host haemoproteins through a haem transporter HmuR and a haemophore HmuY. The aim of this study was to analyse the binding specificity of HmuY towards non-iron metalloporphyrins which may be employed as antimicrobials to treat periodontitis. HmuY binds gallium(iii), zinc(ii), cobalt(iii), manganese(iii), nickel(ii), and copper(ii) protoporphyrin IX but in a manner different to iron(iii) protoporphyrin IX which u...
Various forms of hepatitis C virus (HCV)-related particles are produced from HCV-infected cells. Measuring infectivity of a HCV sample with the conventional 'foci counting method' is laborious and time-consuming. Moreover, the infectivity of a HCV sample does not correlate with the amount of viral RNA that can be measured by real-time RT-PCR. Here we report a new assay suitable for quantifying infectious HCV particles using aptamers against HCV E2, which is named 'Enzyme Linked Apto-Sorbent Assay (ELASA)'....
The effect of raw materials on product yield and quality in bio-manufacturing is considerable and often poorly understood. Here we describe the capabilities of NIR (near-infrared) and two dimensional (2D)-fluorescence spectroscopies in detecting chemical changes over time in two media components (a basal and a feed media) used in Chinese hamster ovary (CHO) cell cultivations for monoclonal antibodies (Mabs) production. For the basal medium both spectroscopies were able to detect compositional changes over s...
BACKGROUND AND AIM: Inflammatory bowel disease is characterized by chronic inflammation of the gastro intestinal tract that manifests as ulcerative colitis and Crohn's disease. Comparative expression profiles of selected ABC transporter genes during active ulcerative colitis and intestinal tuberculosis were studied, and we also investigated the effect of inflammatory modulators on the expression of the transporters in HT-29 cells. METHODS: Using the GEO database, we selected ABC transporter genes that are d...
Chitinolytic enzymes: An appraisal as a product of commercial potential.
Chitin, its deacetylated form, chitosan and chitinolytic enzymes viz. endo-chitinase, N-acetylglucosaminidase, chitosanase, chitin deacetylase are gaining importance for their biotechnological applications. Presently chitin degrading enzymes constitute high-cost low-volume products in human health care and associated research. Indeed chitinases and chitin deacetylase-chitosanase complex possesss tremendous potential in agriculture to control plant pathogenic fungi and insects. The success in exploring chiti...
Heroin activates Bim via c-Jun N-terminal kinase/c-Jun pathway to mediate neuronal apoptosis.
Heroin is reported to cause spongiform leukoencephalopathy (SLE) in heroin addicts and the exact mechanism has not yet been identified. In the present study, we found that heroin could induce apoptosis of primary cultured cerebellar granule cells (CGCs) and Bim was upregulated both transcriptionally and post transcriptionally during CGCs apoptosis. Upregulated Bim translocated to mitochondria and Bax was activated under heroin treatment. Genetic knockdown of Bim using lentiviruses significantly prevented ne...
Commercialisation of Regenerative Medicine: Learning from Spin-Outs.
'Commercialisation of your regenerative medicine research; lessons from spin out successes' was hosted by Oxbridge Biotech Roundtable (OBR) (Oxford, UK) at the University of Oxford in February 2013, attracting a multi-stakeholder audience, spanning academia and industry. The event featured case studies from Gregg Sando, CEO, Cell Medica (London, UK), John Sinden, CSO, Reneuron (Guilford, UK) and Paul Kemp, CEO and CSO, Intercytex (Manchester, UK). OBR is a student led initiative with over 7,000 members acro...
Parkinson's disease (PD) is a neurodegenerative disorder and these days a lot of emphasis is given on the treatment of this disease using herbal medicines. The present study evaluates the neuroprotective effect of Withania somnifera (Ws) root extract on Parkinsonian mice. The mice were divided into three groups; the first group served as control, the second group was given maneb (MB) and paraquat (PQ) and the last group was administered MB-PQ along with Ws root extract for 3, 6 and 9 weeks. The behavioral...
Industry progress report on neuro-oncology: a biotech update.
With steadily rising revenue and large numbers of clinical trials utilizing novel treatment strategies, the field of neuro-oncology is at the core of the growing cancer therapy industry. In June 2012, the Weill Cornell Brain and Tumor Center hosted the first Brain Tumor Biotech Summit as a forum for fostering and encouraging collaboration between researches and investors to accelerate novel treatments for brain cancer. This event brought together neuro-oncologists, neurosurgeons, academicians, entrepreneurs...
This paper describes the development of a HTPD platform for process development of chromatography steps. An assessment of the platform as a guidance tool for establishing the "characterization space" for an ion exchange chromatography step has been performed by using design of experiments (DOE). Case studies involving use of a biotech therapeutic, Granulocyte Colony Stimulating Factor (GCSF), have been used to demonstrate the performance of the platform. We discuss the various challenges that arise when wor...
Collagen sponges (COL-S) are used as scaffolds to support osteoblasts and stimulate bone repair because of their flexibility, biocompatibility, and biodegradability. In this study, we added fibrin networks to COL-S scaffolds by using a fibrinogen (FNG) cross-linking reaction and evaluated the proliferation, differentiation, and adhesion of MG-63 cells on these scaffolds. The fibrin network that formed in COL-S with various concentrations of FNG was characterized with regard to morphology, porosity, and wate...
The minimal zinc hook peptide of Rad50 and its alanine mutants form highly stable Zn(ii) complexes. These peptides were successfully used as a small, efficient tag for reversible Zn(ii)-mediated protein homodimerization. The high stability, its biological consequences and potential applications in protein engineering are discussed.
Lactic acid bacterial extracts as anti-Helicobacter pylori: a molecular approach.
BACKGROUND: Helicobacter pylori (H. pylori) infection, the main cause of chronic gastritis, increases gastric cancer risk. Antibiotics-based H. pylori eradication treatment is 90 % effective. However, it is expensive and causes side effects and antibiotic resistance. Lactic acid bacteria (LAB) could present a low-cost, large-scale alternative solution to prevent or decrease H. pylori colonization. AIM: This work aimed to study the inhibitory effects of LAB strains on the growth and pathogenic activity of H...
Diels-Alder Mediated Controlled Release from a Poly(ethylene glycol) Based Hydrogel.
A synthetic amino acid bearing a furan functionality was developed and incorporated into peptide sequences using solid phase synthesis. Peptides expressing the furan moiety were attached to poly(ethylene glycol) (PEG) hydrogels through a thermally reversible covalent bond formed by a Diels-Alder reaction. Reactions of thiol and maleimide PEG macromers in an off-stoichiometric Michael addition were performed, such that the maleimide moiety was in excess, to create hydrogel networks with pendant Diels-Alder c...
Therapeutic vaccines are currently being developed for chronic hepatitis B and C. As an alternative to long-term antiviral treatment or to support only partially effective therapy, they should activate the patient's immune system effectively to fight and finally control the virus. A paradigm of therapeutic vaccination is the potent induction of T-cell responses against key viral antigens - besides activation of a humoral immune response. We have evaluated the potential of a novel vaccine formulation compris...
Abstract We propose a biotech measurement scheme and software based on new features for the diagnosis of backbone osteochondrosis. Together with the traditional methods of diagnosis, the energy characteristics of biologically active points (acupuncture points, or APs) are used. This new software is based on mathematical models of the internal and biologically active points of meridian structures' interaction. The information from the APs is used in the solving rules based on fuzzy logic for decision-making,...
Measuring frequency domain granger causality for multiple blocks of interacting time series.
In the past years, several frequency-domain causality measures based on vector autoregressive time series modeling have been suggested to assess directional connectivity in neural systems. The most followed approaches are based on representing the considered set of multiple time series as a realization of two or three vector-valued processes, yielding the so-called Geweke linear feedback measures, or as a realization of multiple scalar-valued processes, yielding popular measures like the directed coherence...
Protein Bodies in Nature and Biotechnology.
Protein bodies are natural structures containing protein aggregates that exist in many organisms ranging from bacteria to mammals and plants. In bacteria they are often a phenomenon associated to over-expression of heterologous proteins. In mammals the so called Russell bodies indicate an accumulation of mutated immune globulins. In plants the protein bodies play a major role as protein storage organelle in seeds. Besides these natural cases, protein bodies can also be artificially induced primarily using s...
Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity.
Lactate production is monitored in industrial processes as a crucial metabolite for cultured mammalian cells. Typically lactate is strongly produced during the exponential growth phase, while its net consumption is frequently observed when cells enter into the stationary phase. Such a metabolic shift is desirable because it seems to favor optimal process performance. However, this shift is neither generic nor can it be easily controlled, as the mechanisms modulating lactate production/consumption in cell cu...
An efficient single-enzymatic cascade approach for the asymmetric synthesis of chiral amines has been developed, which applies the amino donor 3-aminocyclohexa-1,5-dienecarboxylic acid spontaneously tautomerizing to reach reaction completion with excellent ee values.
Background:Critical to the continual improvement of cryoablation efficacy is deciphering the biochemical responses of cells to low-temperature exposure. The identification of delayed-onset cell death has allowed for the manipulation of cellular responses through the regulation of apoptosis. We hypothesized that in addition to delayed apoptotic events associated with mild subfreezing temperatures (10 to -25 °C), cells exposed to ultra-low temperatures (
Establishment of bovine embryonic stem cell lines using a minimized feeder cell drop.
Abstract Bovine embryonic stem cells (ESCs) are a powerful tool for agricultural and biomedical applications. The purpose of this study was to introduce a new method for generating bovine ESCs. Mechanically isolated bovine inner cell masses (ICMs) from in vitro-produced blastocysts were cultured individually on a 10-μL mouse embryonic fibroblast (MEF) feeder cell drop covered with oil. From 126 blastocysts classified by their developmental stage and ICM size, 21 primary bovine ESC-like colonies were formed...
The specific infectivity of hepatitis C virus changes through its life cycle.
Hepatitis C virus (HCV) causes liver diseases, such as hepatitis, liver cirrhosis, steatosis, and hepatocellular carcinoma. To understand the life cycle and pathogenesis of HCV, the one-step growth of HCV in a cell culture system was analyzed using a highly infectious variant of the JFH1 clone. The observed profiles of HCV RNA replication indicated that the synthesis of negative-strand RNAs occurred at 6h (h) after infection, followed by the active synthesis of positive-strand RNAs. Our measurements of infe...
