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02:12 EST 18th December 2014 | BioPortfolio

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Showing "UPDATE Roche steps pressure Illumina battle" PubMed Articles 1–25 of 7,100+


Effect on blood pressure of daily lemon ingestion and walking.

Background. Recent studies suggest that the daily intake of lemon (Citrus limon) has a good effect on health, but this has not been confirmed in humans. In our previous studies, it was observed that people who are conscious of their health performed more lemon intake and exercise. An analysis that took this into account was required. Methodology. For 101 middle-aged women in an island area in Hiroshima, Japan, a record of lemon ingestion efforts and the number of steps walked was carried out for five months...

Multiplicative update rules for concurrent nonnegative matrix factorization and maximum margin classification.

The state-of-the-art classification methods which employ nonnegative matrix factorization (NMF) employ two consecutive independent steps. The first one performs data transformation (dimensionality reduction) and the second one classifies the transformed data using classification methods, such as nearest neighbor/centroid or support vector machines (SVMs). In the following, we focus on using NMF factorization followed by SVM classification. Typically, the parameters of these two steps, e.g., the NMF bases/co...

Is confirmatory testing of Roche cobas 4800 CT/NG test Neisseria gonorrhoeae positive samples required? Comparison of the Roche cobas 4800 CT/NG test with an opa/pap duplex assay for the detection of N gonorrhoeae.

Recently marketed nucleic acid amplification tests (NAAT) for the detection of Neisseria gonorrhoeae (NG) have improved specificity over previous generation assays. A study to assess the necessity for confirmation of Roche cobas 4800 NG positive samples was undertaken by the Public Health Wales Microbiology Molecular Diagnostic Unit in Cardiff.

Update on the Guytonian view of hypertension.

Dr Arthur Guyton hypothesized that the capacity of the kidney to excrete sodium ultimately dictates long-term changes in blood pressure. This model has had a profound influence on our understanding of blood pressure regulation. The goal of this article is to review a selection of classic studies and highlight more recent molecular studies supporting or refuting the Guyton model of blood pressure regulation.

Service impact of a change in HIV-1 viral load quantification assay.

Introduction: Due to discontinuation of the Siemens Versant HIV-1 RNA (bDNA) assay in the UK, our laboratory switched to the Roche Cobas Ampliprep/Taqman HIV-1 viral load (VL) assay (Roche) in April 2013. This assay has a lower cut-off of 20 RNA copies/mL (compared with

Metallographic Assessment of Al-12Si High-Pressure Die Casting Escalator Steps.

A microstructural characterization study was performed on high-pressure die cast specimens extracted from escalator steps manufactured from an Al-12 wt.% Si alloy designed for structural applications. Black and white, color light optical imaging and scanning electron microscopy techniques were used to conduct the microstructural analysis. Most regions in the samples studied contained globular-rosette primary α-Al grains surrounded by an Al-Si eutectic aggregate, while primary dendritic α-Al grains were pr...

Pressure-relieving devices for treating heel pressure ulcers.

Pressure ulcers are areas of localised damage to the skin and underlying tissue caused by pressure or shear. Pressure redistribution devices are used as part of the treatment to reduce the pressure on the ulcer. The anatomy of the heel and the susceptibility of the foot to vascular disease mean that pressure ulcers located there require a particular approach to pressure relief.

Serotonin and Sensory Nerves: Meeting in the Cardiovascular System.

Blood pressure regulation by 5-HT has proven to be a complex story to unravel. The work by Cuesta et al., in this issue of Vascular Pharmacology adds another layer of complexity by providing sound in vivo data that 5-HT, through the 5-HT7 receptor, can inhibit the vasodepressor actions of the sensory nervous system and thereby promote blood pressure maintenance. This interaction of 5-HT with the sensory nervous system is inhibitory, whereas 5-HT is understood to be stimulatory in other systems. Moreover, ac...

Comparison of the Gen-Probe Aptima HIV-1 and Abbott HIV-1 qualitative assays with the Roche Amplicor HIV-1 DNA assay for early infant diagnosis using dried blood spots.

The current gold standard for infant diagnosis of HIV-1 is the Roche Amplicor Qualitative DNA assay, but it is being phased out.

Salaries are in a holding pattern, although upward pressure continues on compensation for ED medical directors.

Total Synthesis of Elastin Peptide Using High Pressure-Liquid Phase Synthesis Assisted by a Soluble Tag Strategy.

A highly aggregating elastin peptide was prepared efficiently using a high pressure-liquid phase synthesis approach assisted by a soluble tag strategy. Two standard syringes were connected to each other to construct a reactor. This simple reactor was used to apply high pressure to the highly viscous reaction mixture thereby maintaining its fluidity. The reactions were completely inhibited due to aggregation when conducted in a standard flask reactor, whereas our high pressure approach accelerated the coupli...

If we win a key battle...

The illumina-solexa sequencing protocol for bacterial genomes.

Based on reversible dye-terminators technology, the Illumina-solexa sequencing platform enables rapid sequencing-by-synthesis (SBS) of large DNA stretches spanning entire genomes, with the latest instruments capable of producing hundreds of gigabases of data in a single sequencing run. Illumina's NGS instruments powerfully combine the flexibility of single reads with short- and long-insert paired-end reads, and enable a wide range of DNA sequencing applications. Here, we describe the paired-end library prep...

Effects of cyclic static stretch on fatigue recovery of triceps surae in female basketball players.

Static stretch is a safe and feasible method which usually is used before exercise to avoid muscle injury and to improve muscle performance. The purpose of this study was to determine the effects of cyclic static stretch (CSS) on fatigue recovery of triceps surae (TS) in female basketball players. Nine athlete volunteers between 20 and 30 years participated in this study containing two sessions. After warm-up a pressure cuff was fastened above the knee joint and its pressure was increased to 140 mmHg. The s...

5-Fluorouracil for glaucoma surgery.

Trabeculectomy is performed as a treatment for many types of glaucoma in an attempt to lower the intraocular pressure. The surgery involves creating a channel through the sclera, through which intraocular fluid can leave the eye. If scar tissue blocks the exit of the surgically created channel, intraocular pressure rises and the operation fails. Antimetabolites such as 5-Fluorouracil (5-FU) are used to inhibit wound healing to prevent the conjunctiva scarring down on to the sclera. This is an update of a Co...

Steps Measured by Pedometry and the Relationship to Adiposity in College Women.

Background: The aim of this study was to investigate the relationship between steps per day and adiposity among college women. Methods: This study was cross-sectional and included women ages 18-25. Participants wore a pedometer for 7 consecutive days. Body composition was assessed using air-displacement plethysmography. Height, weight, and waist and hip circumferences were assessed. Results: The women took 10,119 ± 2836 steps per day. When divided into quartiles by steps, the top 2 quartiles of women in th...

PolyP and APC fight a RAGEing battle.

iMSAT: a novel approach to the development of microsatellite loci using barcoded Illumina libraries.

Illumina sequencing with its high number of reads and low per base pair cost is an attractive technology for development of molecular resources for non-model organisms. While many software packages have been developed to identify short tandem repeats (STRs) from next-generation sequencing data, these methods do not inform the investigator as to whether or not candidate loci are polymorphic in their target populations.

Theoretical and practical knowledge of Nursing professionals on indirect blood pressure measurement at a coronary care unit.

Objective To determine and to analyze the theoretical and practical knowledge of Nursing professionals on indirect blood pressure measurement. Methods This cross-sectional study included 31 professionals of a coronary care unit (86% of the Nursing staff in the unit). Of these, 38.7% of professionals were nurses and 61.3% nurse technicians. A validated questionnaire was used to theoretical evaluation and for practice assessment the auscultatory technique was applied in a simulation environment, under a non-p...

Measurement of blood pressure variability and the clinical value.

Authors have collected and analyzed literature data on blood pressure variability. They present the methods of blood pressure variability measurement, clinical value and relationships with target organ damages and risk of presence of cardiovascular events. They collect data about the prognostic value of blood pressure variability and the effects of different antihypertensive drugs on blood pressure variability. They underline that in addition to reduction of blood pressure to target value, it is essential t...

Battle Scars.

Generation of phase-shifting algorithms with N arbitrarily spaced phase-steps.

Phase-shifting (PS) is an important technique for phase retrieval in interferometry (and three-dimensional profiling by fringe projection) that requires a series of intensity measurements with known phase-steps. Usual PS algorithms are based on the assumption that the phase-steps are evenly spaced. In practice, however, this assumption is often not satisfied exactly, which leads to errors in the recovered phase. In this work we present a systematic algebraic approach for generating general PS algorithms wit...

Three Steps Forward and 2 Steps Back: The Echternach Procession Toward Optimal Hydrocephalus Treatment.

Development. Chemical warfare in the battle of the sexes.

South Africa's battle against HIV/AIDS gains momentum.

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