PubMed Journals Articles About "Laboratory"

Is a score of 10 or greater on the Beck Depression Inventory equivalent to clinically diagnosed depression?

Everolimus versus sirolimus-eluting stents for the treatment of sirolimus-eluting stent restenosis.

Delayed eosinophilic gastroenteritis, a possible side effect of clopidogrel?

NQO1 609C>T polymorphism interaction with tobacco smoking and alcohol drinking increases colorectal cancer risk in a Chinese population.

NAD (P)H:quinone oxidoreductase (NQO1) catalyzes the activation of some environmental procarcinogens present in tobacco smoke or the diet. We conducted a hospital-based case-control study to evaluate the potential association between NQO1 609C>T polymorphisms and colorectal cancer risk in a Chinese population.

Genetic structure, polymorphism identification of LGP2 gene and their relationship with the resistance/susceptibility to GCRV in grass carp, Ctenopharyngodon idella.

Laboratory of genetics and physiology 2 (LGP2) is an actual detector and regulator during RNA viral infection in innate immunity. In this study, 5'-flanking region and all introns of LGP2 in grass carp (Ctenopharyngodon idella) were excavated. The genomic CiLGP2 (C. idella LGP2) was 8062bp in length, with a 364bp 5'-flanking region, twelve exons and eleven introns. Besides, the promoter activity of the upstream region before initiator codon was identified. By sequencing, six single nucleotide polymorphisms...

Metagenomic sequencing of "HPV-negative" condylomas detects novel putative HPV types.

Condylomas are caused by human papillomavirus (HPV), but may in rare cases be "negative for HPV" by PCR. Metagenomic sequencing can be used for an unbiased assessment of the presence of virus. Ten swab sample pools, each containing four cases of "HPV-negative" condylomas, were subjected to metagenomic sequencing. One pool contained Molluscum contagiosum. Five pools contained HPV, of which three pools contained novel putative HPV-types. The 12 samples in these three pools were sequenced individually. Six of...

The over-expression of calmodulin from Antarctic notothenioid fish increases cold tolerance in tobacco.

Genes involved in the calcium signalling pathway have a relationship with cold tolerance in many plants. The primary reaction to many different environmental stresses is an increase in the cytoplasmic Ca(2+) concentration. Such variations in the Ca(2+) concentration could change the activity of Ca(2+)-dependent protein functions, further regulating the expression of stress-related genes; therefore, the Ca(2+) signalling pathway is involved in the biological stress reaction. The expression of the calcium-mod...

Relationship between chemokine (C-X-C motif) ligand 12 gene variant (rs1746048) and coronary heart disease: Case-control study and meta-analysis.

The goal of our study is to evaluate the contribution of CXCL12 rs1746048 (hg19, chr10:44775574) to the risk of CHD in Han Chinese, and to summarize its role in CHD through meta-analysis of existing studies among various ethnic groups. Significant association is observed between rs1746048-C and an increased risk of CHD in Han Chinese (χ(2)=5.41, df=1, P=0.02). Post hoc analysis reveals an even stronger association of rs1746048 with the risk of CHD for subjects aged 65years or older (genotype: χ(2)=8.39, d...

Comparing methods for metabolic network analysis and an application to metabolic engineering.

Bioinformatics tools have facilitated the reconstruction and analysis of cellular metabolism of various organisms based on information encoded in their genomes. Characterization of cellular metabolism is useful to understand the phenotypic capabilities of these organisms. It has been done quantitatively through the analysis of pathway operations. There are several in silico approaches for analyzing metabolic networks, including structural and stoichiometric analysis, metabolic flux analysis, metabolic contr...

Tagging SNPs in the ERCC4 gene are associated with gastric cancer risk.

ERCC4 plays an essential role in the nucleotide excision repair (NER) pathway, which is involved in the removal of a wide variety of DNA lesions. To determine whether the ERCC4 tagging SNPs (tSNPs) are associated with risk of gastric cancer, we conducted a hospital-based case-control study of 350 cases and 468 cancer-free controls. In the logistic regression (LR) analysis, we found a significantly decreased risk of gastric cancer associated with the rs744154 GC/CC genotypes [adjusted odds ratio (OR)=0.56, 9...

The complete mitochondrial genome sequence analysis of Tibetan argali (Ovis ammon hodgsoni): Implications of Tibetan argali and Gansu argali as the same subspecies.

The genus Ovis (Bovidae, Artiodactyla) includes six species, i.e. Ovis ammon, Ovis aries, Ovis canadensis, Ovis dalli, Ovis nivicola and Ovis vignei. Based on morphology, geographical location, habitat, etc., the species O. ammon is divided into nine subspecies. The near threatened Tibetan argali is distributed across the Tibetan Plateau and its peripheral mountains, and believed to be one of the O. ammon subspecies (O. a. hodgsoni). However, considering its morphological features and distributions, a quest...

Methylenetetrahydrofolate reductase C677T variant in Moroccan patients with inflammatory bowel disease.

The association of genetic polymorphisms related to metabolism of homocysteine and folate with inflammatory bowel disease has been evidenced. Several studies have identified genetic variants of MTHFR as significant susceptibility loci for Crohn's disease (CD) and ulcerative colitis (UC). The C677T genetic polymorphism in the MTHFR gene is found to be associated with a thermolabile variant enzyme that shows a reduced activity. Therefore, we investigated whether the C677T variant confers genetic susceptibilit...

(1)H NMR-based metabonomic analysis of the serum and urine of rats following subchronic exposure to dichlorvos, deltamethrin, or a combination of these two pesticides.

Metabonomic analysis, clinical chemical analysis and histopathology were used to investigate the toxic effects of subchronic exposure to dichlorvos, deltamethrin, and a combination of these two pesticides, in rats. Weight loss, hind limb weakness and histopathological changes in kidney tissue were only observed in rats exposed to high doses of deltamethrin, or a combination of deltamethrin and dichlorvos. Urinary metabonomic analysis indicated that exposure to a mixture of dichlorvos and deltamethrin was fo...

Small sized perylene-bisimide assemblies controlled by both cooperative and anti-cooperative assembly processes.

Cooperative aggregation of monomers into one-dimensional nanostructures typically results in elongated objects. Here we analyse in depth the self-assembly of an N-monoarylated perylene bisimide which shows characteristics of a cooperative growth mechanism but unexpectedly yields objects of small size, due to anti-cooperativity by attenuated growth.

Quantitative IR Spectrum and Vibrational Assignments for Glycolaldehyde Vapor: Glycolaldehyde Measurements in Biomass Burning Plumes.

Glycolaldehyde (GA, 2-hydroxyethanal, C2H4O2) is a semivolatile molecule of atmospheric importance, recently proposed as a precursor in the formation of aqueous-phase secondary organic aerosol (SOA). There are few methods to measure GA vapor, but infrared spectroscopy has been used successfully. Using vetted protocols we have completed the first assignment of all fundamental vibrational modes and also derived quantitative IR absorption band strengths using both neat and pressure-broadened GA vapor. Even tho...

Photoelectron Spectroscopic and Theoretical Study of Aromatic-Bim Anionic Complexes (Aromatic = C6H5, C5H4N, C4H3O, and C4H4N; m = 1-3): A Comparative Study.

The reactions between Bi clusters generated by laser ablation and different aromatic molecules (C6H6, C5H5N, C4H4O, or C4H5N) seeded in argon carrier gas were studied by a reflectron time-of-flight mass spectrometer (RTOF-MS) with a photoelectron spectrometer. The photoelectron (PE) spectra of the dominant anionic products BimC6H5(-), BimC5H4N(-) (m = 1-4) and BimC4H3O(-), BimC4H4N(-) (m = 1-3) dehydrogenated complexes were obtained by 308 and 193 nm laser, respectively. It was found that the adiabatic elec...

Increased Growth of a Hydrogenotrophic Methanogen in Co-Culture with a Cellulolytic Bacterium under Cathodic Electrochemical Regulation.

Bioelectrochemical (-0.8 V, -0.3 V, and +0.6 V vs. Ag/AgCl) and non-bioelectrochemical co-cultures of a hydrogenotrophic methanogen and a cellulolytic bacterium were conducted. Unlike non-bioelectrochemical co-cultures, a cathodic reaction (-0.8 V) increased the growth of the hydrogenotrophic methanogen and the cellulolytic bacterium, by 6.0- and 2.2-fold respectively, and increased cellulose degradation. In contrast, anodic reactions (-0.3 V, +0.6 V) influenced them negatively.

Assessment of Key Amino-Acid Residues of CD36 in Specific Binding Interaction with an Oxidized Low-Density Lipoprotein.

CD36 binds oxidized low-density lipoprotein (oxLDL). A synthetic peptide comprising amino-acid residues 149-168 of mouse CD36 was recently found to bind fluorescence-labeled oxLDL particles. Based on our oxLDL-binding analysis of various synthetic CD36 peptides, we suggest that not only hydrophilic residues (e.g., Lys164 and Lys166) but also hydrophobic ones (e.g., Phe153, Leu158, and Leu161) are critical to binding.

Identification of Alkylbenzene Sulfonate Surfactants Leaching from an Acrylonitrile Butadiene Rubber as Novel Inhibitors of Calcineurin Activity.

Calcineurin (CN) is a Ca(2+)/calmodulin (CaM) dependent serine/threonine protein phosphatase and plays important role in several cellular functions in both higher and lower eukaryotes. Here we report inhibition of CN by linear alkylbenzene sulfonate. The clue to the finding was obtained while identifying the inhibitory material leaching from acrylonitrile butadiene rubber used for packing. Using standard dodecylbenzene sulfonate (C12-LAS), we obtained strong inhibition of CN with a half maximal inhibitory c...

Studies Targeting α-Glucosidase Inhibition, Antiangiogenic Effects, and Lipid Modification Regulation: Background, Evaluation, and Challenges in the Development of Food Ingredients for Therapeutic Purposes.

Since the discovery of α-glucosidase inhibitors and their inhibitory effects on the digestion of carbohydrates, promising results have been obtained as to the antidiabetic effects of this family of compounds. Antiangiogenic compounds have been identified that suppress tumor growth via a unique mechanism, confirming that such compounds can act as clinically applicable anticancer agents. Lipid peroxidation and lipid glycation have been suggested to play roles in food deterioration and in the pathophysiology...

Saving Specimens after Sandy.

When Hurricane Sandy wiped out the power to the VA in Manhattan, 25 years' worth of specimens held by researchers working on potential HIV vaccines and tuberculosis diagnostics were in jeopardy. But the laboratory team swung into action to preserve their frozen assets.

Remote-control photocycloreversion of dithienylethene driven by strong push-pull azo chromophores.

In this communication, a novel photochromic molecular switch DTE-2(Azo-CN) containing both dithienylethene and push-pull azo chromophores was synthesized. The push-pull azo chromophores can significantly accelerate the ring opening of the C-C bond of DTE closed isomer when irradiated with light far away from the maximum absorption band of the DTE closed isomer.

Assembly of DNA-functionalized gold nanoparticles on electrospun nanofibers as a fluorescent sensor for nucleic acids.

A facile approach was developed to assemble DNA-functionalized gold nanoparticles on the electrospun nanofibers. This novel nanocomposite was successfully applied to constructing a highly sensitive and selective fluorescent sensor for nucleic acids.

LnN(SiMe3)23-catalyzed cycloaddition of terminal alkynes to azides leading to 1,5-disubstituted 1,2,3-triazoles: new mechanistic features.

The first example of rare earth metal-catalyzed cycloaddition of terminal alkynes to azides resulting in the formation of 1,5-disubstituted 1,2,3-triazoles is described. Preliminary studies revealed that the present cycloaddition shows unprecedented mechanistic features involving a tandem anionic cascade cyclization and anti-addition across the C[triple bond, length as m-dash]C triple bond.

Imatinib results in better outcomes than HLA-identical sibling transplants in young persons with newly-diagnosed chronic phase chronic myelogenous leukemia.