Chromogranin-A-Derived Peptides and Periodontitis in Diabetic Patients
Summary
Periodontitis is a chronic disease of the supporting tissue of the teeth, characterized by bleeding and inflamed gingival, resorption of alveolar bone. This disease has a significant impact of the quality of life as the teeth become progressively mobile and may fall out spontaneously if no treatment is provided. Periodontal disease has been cited as the sixth complication of diabetes mellitus.Chromogranins / secretogranins are a family of proteins present in nervous endocrine and immune cells, released with catecholamines during stress situations. Chromogranin A (CGA) is the major member of this family.The aim of this study is to determine the level of CGA and its derived peptides in the gingival crevicular fluid (GCF) of health or diabetic patients with and without periodontitis.Then, we want to determine the antimicrobial activity of these peptides.
Study Design
Allocation: Non-Randomized, Control: Uncontrolled, Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Diagnostic
Conditions
Periodontitis and Diabetitis
Intervention
Gingival crevicular fluid samples (this procedure is added to the subject usual medical follow-up).
Location
Département de Parodontologie - Hôpital Civil
Strasbourg
France
67091
Status
Recruiting
Source
University Hospital, Strasbourg, France
Results (where available)
Links
- Source: http://clinicaltrials.gov/show/NCT00399620
- Information obtained from ClinicalTrials.gov on July 15, 2010
Medical and Biotech [MESH] Definitions
Gingival Crevicular Fluid
A fluid occurring in minute amounts in the gingival crevice, believed by some authorities to be an inflammatory exudate and by others to cleanse material from the crevice, containing sticky plasma proteins which improve adhesions of the epithelial attachment, have antimicrobial properties, and exert antibody activity. (From Jablonski, Illustrated Dictionary of Dentistry, 1982)
Gingival Overgrowth
Excessive growth of the gingiva either by an increase in the size of the constituent cells (GINGIVAL HYPERTROPHY) or by an increase in their number (GINGIVAL HYPERPLASIA). (From Jablonski's Dictionary of Dentistry, 1992, p574)
Chromatography, Supercritical Fluid
A CHROMATOGRAPHY method using supercritical fluid, usually carbon dioxide under very high pressure (around 73 atmospheres or 1070 psi at room temperature) as the mobile phase. Other solvents are sometimes added as modifiers. This is used both for analytical (SFC) and extraction (SFE) purposes.
Gingival Pocket
An abnormal extension of a gingival sulcus not accompanied by the apical migration of the epithelial attachment.
Pyrococcus Horikoshii
Anaerobic hyperthermophilic species of ARCHAEA, isolated from hydrothermal fluid samples. It is obligately heterotrophic with coccoid cells that require TRYPTOPHAN for growth.
Clinical Trials
Gingival Wetness and Gingival Crevicular Fluid Volume in Patients With Dry Mouth
The investigators questioned whether patients with dry mouth complaints display any reduction in residual saliva coating the gingiva and other selected mucosal surfaces. We further intende...
Collection of Gingival Crevicular Fluid From Periodontitis Patients
To collect GCF samples from diseased patients suffering only from gingivitis and/or periodontitis.
We hypothesize that Asian Americans compared to Caucasians, will be at higher risk of developing a pro-inflammatory state that may contribute to the development of heart disease and diabet...
Integrated Microfluidic System for Oral Diagnostics
This study is being conducted to test the ability and accuracy of a new instrument in the diagnosis of periodontitis (gum disease) by using fluids from the mouth. The diagnosis instrumen...
Genes Associated With Hereditary and Drug-Induced Gingival Overgrowth
This study will examine common features of gingival overgrowth (excessive growth of the gums around the teeth) that develops in patients with the hereditary form of the condition and in th...
PubMed Articles
Insulin-like growth factor-binding protein-2 and -3 in gingival crevicular fluid.
Takenouchi Y, Ohshima M, Yamaguchi Y, Nishida T, Senda N, Idesawa M, Otsuka K, Ito K. Insulin-like growth factor-binding protein-2 and -3 in gingival crevicular fluid. J Periodont Res 2010; doi: 10.11...
The gingival crevicular fluid (GCF) contains various biomarkers, such as interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor-α (TNF-α), and IL-10, among others. These cytokines have been reporte...
Full-mouth profile of active MMP-8 in periodontitis patients.
Kraft-Neumärker M, Lorenz K, Koch R, Hoffmann T, Mäntylä P, Sorsa T, Netuschil L. Full-mouth profile of active MMP-8 in periodontitis patients. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.0...
Effects of periodontal therapy on GCF cytokines in generalized aggressive periodontitis subjects.
AIM: To examine changes in levels of gingival crevicular fluid (GCF) cytokines, after periodontal therapy of generalized aggressive periodontitis (GAgP). MATERIALS AND METHODS: Twenty-five periodontal...
The aim of this study was to evaluate the long-term effects of a combined periodontal treatment of scaling and root planing (SRP) and Nd:YAG laser (NDL) in chronic periodontitis (CP) patients. This wa...
