Adenosine Receptors Influence Ischemia-Reperfusion Injury

15:27 EST 28th November 2014 | BioPortfolio

Summary

Ischemic preconditioning is defined as the development of tolerance to ischemia-reperfusion injury by a previous short bout of ischemia resulting in a marked reduction in infarct size. This mechanism can be mimicked by several pharmacological substances such as acetylcholine and adenosine.

To detect ischemia-reperfusion injury in humans in vivo Kharbanda et al. developed a method in which endothelial dysfunction represents the effects of ischemic preconditioning. This method, however, uses acetylcholine to measure endothelial function before and after forearm ischemia. We, the investigators at Radboud University, hypothesize that the use of acetylcholine in this model reduces ischemia-reperfusion injury. Therefore, we will compare this protocol with a protocol in which endothelial function is only measured after ischemia. We expect an increase in ischemia-reperfusion injury when endothelial function is only measured after the forearm ischemia.

After determining the optimal method to measure ischemia-reperfusion injury of the vascular endothelium we will determine the effect of acute and chronic caffeine, an adenosine receptor antagonist, on ischemic preconditioning. With this study we expect to find that adenosine mimics ischemic preconditioning of the vascular endothelium. Moreover, we expect to find that acute caffeine intake reduces ischemia-reperfusion injury whereas chronic caffeine intake does not. This study will increase our knowledge about the mechanism of ischemic preconditioning and may also provide leads to exploit this endogenous protective mechanism in a clinical setting.

Study Design

Allocation: Randomized, Control: Placebo Control, Intervention Model: Crossover Assignment, Masking: Double-Blind

Conditions

Ischemia-Reperfusion Injury

Intervention

acetylcholine, twenty minutes of forearm ischemia, three 5-minute periods of forearm ischemia, adenosine, caffeine

Location

Radboud University Nijmegen Medical Centre/Department of Pharmacology and Toxicology
Nijmegen
Gelderland
Netherlands
6500 HB

Status

Suspended

Source

Radboud University

Results (where available)

View Results

Links

Clinical Trials [405 Associated Clinical Trials listed on BioPortfolio]

Effect of Ischemic Postconditioning on Targeting of Annexin A5 After Forearm Exercise

To adjust our forearm model for ischemia reperfusion injury, in order to used it for assessment of ischemia reperfusion damage in elderly and patients. To test the effect of ischemic post...

The Effect of Caffeine on Ischemic Preconditioning

Ischaemic preconditioning (IP) describes the phenomenon that brief periods of ischaemia render the (myocardial) muscle more resistant to a subsequent more prolonged period of ischaemia and...

Opioid Receptors Influence Ischemia-Reperfusion Injury

The most powerful protective mechanism against ischemia-reperfusion injury other than rapid reperfusion is ischemic preconditioning. Ischemic preconditioning is defined as the development ...

Model System for Transient Forearm Blood Vessel Dysfunction

This study will develop a model system that can be used to test medications for improving the ability of blood vessels to resist damage from diseases such as heart attack and stroke. The e...

The Effects of Preconditioning on Intramuscular High-Energy Phosphate Levels During Ischemia

Ischemia reperfusion injury may be reduced by ischemic preconditioning. This projects aims to show the effects of short and long time ischemic preconditioning (both sequences 3 x 5 minutes...

PubMed Articles [5853 Associated PubMed Articles listed on BioPortfolio]

The effect of Interchange rotation period and number on Australian Football running performance.

To determine the effect of on-field rotation periods and total number of game rotations on Australian Football running performance, elite Australian Football players (n=21, mean ± SD; 23.2 ± 1.7 y; ...

Effects of Preoperative Intravenous Clonidine in Patients Undergoing Cataract Surgery: A Double-Blind, Randomized Trial.

Objectives. The aim of this study was to assess the effects of clonidine on intraoperative analgesia, sedation, intraocular and blood pressure, arrhythmia, and ischemia. Methods. Forty patients underg...

THE IMPACT OF EXTENDED WARM ISCHEMIA TIME ON LATE RENAL FUNCTION AFTER ROBOTIC PARTIAL NEPHRECTOMY.

Purpose: To evaluate the effect of warm ischemia time (WIT) on late renal function in patients undergoing robotic partial nephrectomy (RPN). Materials and Methods: From January 2009 to June 2013, pati...

A feasible strategy for focal cerebral ischemia-reperfusion injury: remote ischemic postconditioning.

It is difficult to control the degree of ischemic postconditioning in the brain and other ischemia-sensitive organs. Remote ischemic postconditioning could protect some ischemia-sensitive organs throu...

Enhanced Cardiomyocyte Volume Regulation: A Key Mechanism in Local and Remote Ischemic Preconditioning.

We have previously shown that ischemic preconditioning (IPC) protection against necrosis in whole hearts and both fresh and cultured cardiomyocytes, as well as improved regulatory volume decrease (RVD...

Medical and Biotech [MESH] Definitions

Exposure of myocardial tissue to brief, repeated periods of vascular occlusion in order to render the myocardium resistant to the deleterious effects of ISCHEMIA or REPERFUSION. The period of pre-exposure and the number of times the tissue is exposed to ischemia and reperfusion vary, the average being 3 to 5 minutes.

The branch of medicine dealing with the fetus and infant during the perinatal period. The perinatal period begins with the twenty-eighth week of gestation and ends twenty-eight days after birth. (From Dorland, 27th ed)

A technique in which tissue is rendered resistant to the deleterious effects of prolonged ischemia and reperfusion by prior exposure to brief, repeated periods of vascular occlusion. (Am J Physiol 1995 May;268(5 Pt 2):H2063-7, Abstract)

An acylated inactive complex of streptokinase and human lysine-plasminogen. After injection, the acyl group is slowly hydrolyzed, producing an activator that converts plasminogen to plasmin, thereby initiating fibrinolysis. Its half-life is about 90 minutes compared to 5 minutes for TPA; (TISSUE PLASMINOGEN ACTIVATOR); 16 minutes for UROKINASE-TYPE PLASMINOGEN ACTIVATOR and 23 minutes for STREPTOKINASE. If treatment is initiated within 3 hours of onset of symptoms for acute myocardial infarction, the drug preserves myocardial tissue and left ventricular function and increases coronary artery patency. Bleeding complications are similar to other thrombolytic agents.

Vesicular amine transporter proteins that transport the neurotransmitter ACETYLCHOLINE into small SECRETORY VESICLES. Proteins of this family contain 12 transmembrane domains and exchange vesicular PROTONS for cytoplasmic acetylcholine.

More From BioPortfolio on "Adenosine Receptors Influence Ischemia-Reperfusion Injury"

Search BioPortfolio:
Loading
Advertisement

Relevant Topics

Top five promising drugs Phase I (November 2013)
Latest News Clinical Trials Research Drugs Reports Corporate
THE FIVE MOST PROMISING DRUGS ENTERING PHASE I TRIALS Drug Disease Company ARC-520 Hepatitis B virus infection Arrowhead Research GR-MD-02 Non-alcoholic steatohepatitis ...

Alzheimer's Disease
Latest News Clinical Trials Research Drugs Reports Corporate
Of all the types of Dementia, Alzheimer's disease is the most common, affecting around 465,000 people in the UK. Neurons in the brain die, becuase  'plaques' and 'tangles' (mis-folded proteins) form in the brain. People with Al...

Advertisement