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Search Results for "Agroinfiltration"

04:20 EDT 26th May 2013 | BioPortfolio

Original Source: Agroinfiltration as a technique for rapid assays for evaluating candidate insect resistance transgenes in plants.

Functional analysis of candidate transgenes for insect resistance in stably transformed plants is a time-consuming task that can take months to achieve in even the fastest of plant models. In this study, a rapid screening technique is described, which employs candidate transgene transient expression using agroinfiltration in Nicotiana benthamiana combined with a simple insect bioassay. Using this system the known insecticidal protein Cry1Ac is demonstrated to effectively control Helicoverpa zea. Insects fed...

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Transient silencing of the grapevine gene VvPGIP1 by agroinfiltration with a construct for RNA interference.

Grapevine is an economically important crop, and the recent completion of its genome makes it possible to study the function of specific genes through reverse genetics. However, the analysis of gene f...

Agroinfiltration as a technique for rapid assays for evaluating candidate insect resistance transgenes in plants.

Functional analysis of candidate transgenes for insect resistance in stably transformed plants is a time-consuming task that can take months to achieve in even the fastest of plant models. In this stu...

The pEAQ vector series: the easy and quick way to produce recombinant proteins in plants.

The pEAQ vectors are a series of plasmids designed to allow easy and quick production of recombinant proteins in plants. Their main feature is the use of the Cowpea Mosaic Virus hypertranslational "CP...

An Arabidopsis RNase III-like protein, AtRTL2, cleaves double-stranded RNA in vitro.

Class 1 ribonuclease III (RNase III), found in bacteria and yeast, is involved in processing functional RNA molecules such as ribosomal RNAs (rRNAs). However, in Arabidopsis thaliana, the lack of an o...

Subcellular localization of proteins of Oryza sativa L. in the model tobacco and tomato plants.

The cellular localization and molecular interactions are indicative of functions of a protein. The development of a simple and efficient method for subcellular localization of a protein is indispensab...

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