Biotin and Pantothenic Acid

The AAPS Journal: Functional and Molecular Aspects of Biotin Uptake via SMVT in Human Corneal Epithelial (HCEC) and Retinal Pigment Epithelial (D407) Cells

Type: Original PaperSodium-dependent multivitamin transporter (SMVT) is a vital transmembrane protein responsible for translocating biotin and other essential cofactors such as pantothenate and lipoat...

Alkynes and Nitriles

Put this one in the category of "reactions you probably wouldn't have thought of". There's a new paper in Organic Letters on cleaving a carbon-carbon triple bond, yielding the two halves as their own...

EMBL Chemical Biology: Greasy Labels

Just to emphasize how careful you have to be with all these probes and labels, consider what I'm hearing now from Remigiusz Serwa of the Tate group at Imperial College. His group is looking at farnesy...

Double functionalisation of titanium-based nanoparticles for drug delivery

Scientists in India report the functionalisation of TiO2 nanoparticles with two different biomolecules – tris-(nitrilo tris-acetic acid) and biotin. The biocompatible nanoparticles is non-cytotoxic...

Molecular cloning of a novel bioH gene from an environmental metagenome encoding a carboxylesterase with exceptional tolerance to organic solvents

Background: BioH is one of the key enzymes to produce the precursor pimeloyl-ACP to initiate biotin biosynthesis de novo in bacteria. To date, very few bioH genes have been characterized. In this stud...

EMBL Chemical Biology: Labeling Proteins

Jason Chin of the MRC Molecular Biology lab in the UK has been talking here about protein labeling and genetic code expansion, an overview of the numerous papers his group has been publishing in this...

Singular phase nano-optics in plasmonic metamaterials for label-free single-molecule detection

A suitably engineered plasmonic metamaterial featuring topologically protected sharp phase variations close to a zero-reflection point of incident lightwaves has now been demonstrated. Exploiting the...

Pharmaceutical Research: The Effect of Metal-Chelating Polymers (MCPs) for 111In Complexed via the Streptavidin-Biotin System to Trastuzumab Fab Fragments on Tumor and Normal Tissue Distribution in Mice

Type: Original PaperPurposeTo study the effects of backbone composition and charge of biotin-functionalized metal-chelating polymers (Bi-MCPs) for 111In complexed to streptavidin (SAv)-trastuzumab Fab...

How the biotin-streptavidin interaction was made even stronger: investigation via crystallography and a chimeric tetramer.

The interaction between streptavidin and biotin is one of the strongest non-covalent interactions in nature. Streptavidin is a widely used tool and a paradigm for protein-ligand interactions. We recen...

Clickable enzyme-linked immunosorbent assay.

Click chemistry is explored as a potential cost-effective and selective immobilization method for the production of an enzyme-linked immunosorbent assay (ELISA). Coatings were formulated containing ei...

Development and evaluation of lipid microbubbles targeted to alpha(v)beta(3)-integrin via biotin-avidin bridge.

This work was to report preparation and quality control of targeted microbubbles (MB(t)) via biotin-avidin bridge, specifically verification of the "biotin-avidin-biotin" sandwich structure. (1) Lipid...

Cleavable Biotin Probes for Labeling of Biomolecules via Azide-Alkyne Cycloaddition.

The azide-alkyne cycloaddition provides a powerful tool for bio-orthogonal labeling of proteins, nucleic acids, glycans, and lipids. In some labeling experiments, e.g., in proteomic studies involving...

Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots.

The valency of quantum dot nanoparticles conjugated with biomolecules is closely related to their performance in cell tagging, tracking, and imaging experiments. Commercially available streptavidin co...