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Search Results for "Nattokinase And Purification"

03:36 EDT 19th May 2013 | BioPortfolio

Original Source: Purification and biochemical characterization of a nattokinase by conversion of shrimp shell with Bacillus subtilis TKU007.

BSN1, a nattokinase, was purified from the culture supernatant of Bacillus subtilis TKU007 with shrimp shell wastes as the sole carbon/nitrogen source. The BSN1 was purified to homogeneity by three-step procedure with a 515-fold increase in specific activity and 12% recovery. The molecular masses of BSN1 determined by SDS-PAGE and gel filtrations were approximately 30kDa and 28kDa, respectively. The results of peptide mass mapping showed that four tryptic peptides of BSN1 were identical to the nattokinase f...

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Matching PubMed Articles

In vivo Evaluation Method of the Effect of Nattokinase on Carrageenan-Induced Tail Thrombosis in a Rat Model.

Thrombosis is characterized by congenital and acquired procatarxis. Nattokinase inhibits thrombus formation in vitro. However, in vivo evaluation of the therapeutic efficacy of nattokinase against thr...

Production of nattokinase by batch and fed-batch culture of Bacillus subtilis.

Nattokinase was produced by batch and fed-batch culture of Bacillus subtilis in flask and fermentor. Effect of supplementing complex media (peptone, yeast extract, or tryptone) was investigated on the...

Purification and biochemical characterization of a nattokinase by conversion of shrimp shell with Bacillus subtilis TKU007.

BSN1, a nattokinase, was purified from the culture supernatant of Bacillus subtilis TKU007 with shrimp shell wastes as the sole carbon/nitrogen source. The BSN1 was purified to homogeneity by three-st...

Optimisation of preparation conditions and properties of phytosterol liposome-encapsulating nattokinase.

Phytosterol liposomes were prepared using the thin film method and used to encapsulate nattokinase (NK). In order to obtain a high encapsulation efficiency within the liposome, an orthogonal experimen...

System Establishment of ATPS for One-Step Purification of Glutamate Decarboxylase from E. coli After Cell Disruption.

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