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Circadian topology of metabolism

Biological clocks are genetically encoded oscillators that allow organisms to anticipate changes in the light–dark environment that are tied to the rotation of Earth. Clocks enhance fitness and grow...

Oxygenase-catalyzed ribosome hydroxylation occurs in prokaryotes and humans

The finding that oxygenase-catalyzed protein hydroxylation regulates animal transcription raises questions as to whether the translation machinery and prokaryotic proteins are analogously modified. Es...

Natural GMOs Part 149. Moss Harbors Foreign Genes

Land plants emerged around half a billion years ago, having evolved from green aquatic algae. Today, a representative of these early land-dwelling species—a moss—hints that genes from other kingdo...

Predominant archaea in marine sediments degrade detrital proteins

Half of the microbial cells in the Earth’s oceans are found in sediments. Many of these cells are members of the Archaea, single-celled prokaryotes in a domain of life separate from Bacteria and Euk...

Structure of a bacterial energy-coupling factor transporter

The energy-coupling factor (ECF) transporters constitute a novel family of conserved membrane transporters in prokaryotes that have a similar domain organization to the ATP-binding cassette transporte...

Structural insights into electron transfer in caa3-type cytochrome oxidase

Cytochrome c oxidase is a member of the haem copper oxidase superfamily (HCO). HCOs function as the terminal enzymes in the respiratory chain of mitochondria and aerobic prokaryotes, coupling molecula...

Structure of the proton-gated urea channel from the gastric pathogen Helicobacter pylori

Half the world’s population is chronically infected with Helicobacter pylori, causing gastritis, gastric ulcers and an increased incidence of gastric adenocarcinoma. Its proton-gated inner-membrane...

Crystal structure of a folate energy-coupling factor transporter from Lactobacillus brevis

ATP-binding cassette (ABC) transporters, composed of importers and exporters, form one of the biggest protein superfamilies that transport a variety of substrates across the membrane, powered by ATP h...

Microtubule-assisted mechanism for functional metabolic macromolecular complex formation.

Evidence has been presented for a metabolic multienzyme complex, the purinosome, that participates in de novo purine biosynthesis to form clusters in the cytoplasm of living cells under purine-deplete...

The purinosome, a multi-protein complex involved in the de novo biosynthesis of purines in humans.

Purine nucleotides are ubiquitous molecules that play vital roles in all kingdoms of life, not only as components of nucleic acids, but also participating in signaling and energy storage. Cellular poo...

Evolutionary dynamics of spliceosomal intron revealed by in silico analyses of the P-Type ATPase superfamily genes.

It has been long debated whether spliceosomal introns originated in the common ancestor of eukaryotes and prokaryotes. In this study, we tested the possibility that extant introns were inherited from...

Do Prokaryotes have more kinetically stable proteins than eukaryotic organisms?

Upon folding, some proteins become conformationally trapped, presumably to protect against aggregation or premature degradation. To probe the occurrence of this property, known as kinetic stability, a...

Occurrence of L-iduronic acid and putative D-glucuronyl C5-epimerases in prokaryotes.

Glycosaminoglycans (GAGs) are polysaccharides that are typically present in a wide diversity of animal tissue. Most common GAGs are well-characterized and pharmaceutical applications exist for many of...