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Search Results for "Repeated Cycle Of Freez And Thaw"

01:48 EDT 20th April 2014 | BioPortfolio

Original Source: Repeated freeze-thaw cycles reduce the survival rate of osteocytes in bone-tendon constructs without affecting the mechanical properties of tendons.

Frozen bone-patellar tendon bone allografts are useful in anterior cruciate ligament reconstruction as the freezing procedure kills tissue cells, thereby reducing immunogenicity of the grafts. However, a small portion of cells in human femoral heads treated by standard bone-bank freezing procedures survive, thus limiting the effectiveness of allografts. Here, we characterized the survival rates and mechanisms of cells isolated from rat bones and tendons that were subjected to freeze-thaw treatments, and eva...

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A procedure for the in vivo determination of enzyme activity in higher plant tissue.

Rapid freezing of higher plant tissue in liquid nitrogen renders the cells permeable to a wide range of substrate molecules. Tissue permeabilized by repeated freeze-thaw treatment can be used for the...

Repeated ovarian stimulation does not affect the expression level of proteins involved in cell cycle control in mouse ovaries and fallopian tubes.

To understand if repeated cycles (2-4 rounds) of gonadotropin stimulation could affect intracellular localization/content of proteins controlling cell cycle progression in mouse fallopian tubes (FT) a...

Comparison of VO2 Peak during Treadmill and Cycle Ergometry in Severely Overweight Youth.

The purpose of this study was to compare peak cardiorespiratory parameters during treadmill and cycle ergometry in severely overweight youth. Twenty-one participants from the Committed to Kids Pediatr...

Colloid-facilitated mobilization of metals by freeze-thaw cycles.

The potential of freeze-thaw cycles to release colloids and colloid-associated contaminants into water is unknown. We examine the effect of freeze-thaw cycles on the mobilization of cesium and stronti...

Effect of the holding time at 15°C prior to cryopreservation, the thawing rate and the post-thaw incubation temperature on the boar sperm quality after cryopreservation.

The aim of the present study was to evaluate the effect of the holding time at 15°C prior to cryopreservation (2, 4 and 8h), thawing rate (37°C for 20s or 70°C for 8s) and post-thaw incubation temp...

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