A spectroscopic method to determine the activity of the ...
A spectroscopic method to determine the activity of the restriction endonuclease EcoRV that involves a single reaction. Huang Q, Quiñones E. ...
http://www.ncbi.nlm.nih.gov/pubmed/18489897...
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Sliding and jumping of single EcoRV restriction enzymes on non ...
The restriction endonuclease EcoRV can rapidly locate a short recognition ...... enzyme activity reveals mechanochemical properties of the reaction pathway. ...
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=24...
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The cytotoxic domain of colicin E9 is a channel-forming ...
Endonuclease activity was assayed in 50 mM triethanolamine buffer, pH 7.5, .... S.E. Rapid reaction analysis of the catalytic cycle of the EcoRV restriction ...
http://www.nature.com/nsmb/journal/v9/n6/full/nsb797.html...
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Dynamics and consequences of DNA looping by the FokI restriction ...
Perona JJ. Type II restriction endonucleases. Methods. 2002;28:353–364. ... Rapid-reaction analysis of plasmid DNA cleavage by the EcoRV restriction ...
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=23...
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The junction-resolving enzymes : Article : Nature Reviews ...
-sheet in RuvC compared with the restriction endonucleases have led to the suggestion .... This is the most extreme case found so far, but spectroscopic
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http://www.nature.com/nrm/journal/v2/n6/full/nrm0601_433a.ht...
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Structure and function of type II restriction endonucleases
Erskine S.G. and Halford,S.E. (1998) Reactions of the EcoRV restriction ..... homodimeric type II restriction endonuclease: single-chain PvuII endonuclease.
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http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=55...
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Synthesis of the 3[prime]-thio-nucleosides and subsequent ...
6 Dec 2007 ... the restriction endonuclease EcoRV1, 3, 4; exonucleolytic activity ... prepared using methods based on a Michaelis–Arbuzov reaction5, 6, ...
http://www.nature.com/nprot/journal/v2/n12/full/nprot.2007.4...
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A real-time assay for monitoring nucleic acid cleavage by ...
Perona J.J. Type II restriction endonucleases. Methods. 2002;28:353–364. ... S.E. Rapid-reaction analysis of plasmid DNA cleavage by the EcoRV restriction
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http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=16...
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Structure of the bacteriophage T4 DNA adenine methyltransferase ...
2b), invariant residues that have been implicated in specific EcoRV-DNA ... resulting in resistance to cleavage by the R.EcoP1 restriction endonuclease2.
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http://www.nature.com/nsmb/journal/v10/n10/full/nsb973.html...
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Non-additivity of sequence-specific enzyme-DNA interactions in the ...
To determine the extent to which structural changes in the DNA alone contribute to this lack .... Interaction of the EcoRV restriction endonuclease with the ...
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=32...
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